{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T14:10:33Z","timestamp":1774620633319,"version":"3.50.1"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1998,10,1]],"date-time":"1998-10-01T00:00:00Z","timestamp":907200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>There is a need for rapid and sensitive detection of Mycobacterium tuberculosis in tissue specimens. A polymerase chain reaction (PCR)-based assay for the diagnosis of tuberculosis was evaluated in 60 formalin-fixed tissue specimens, the target for the amplification being a segment of IS6110 in the M. tuberculosis chromosome. Of the 60 formalin-fixed, paraffin-embedded tissue specimens studied, 57 showed granulomatous inflammation and 53 had been cultured for mycobacteria; 10 were positive for M. tuberculosis and three were positive for other mycobacteria. Of 60 samples, 15 showed acid-fast bacilli on special staining. When done comparatively on a positive culture for M. tuberculosis, PCR for M. tuberculosis DNA in 60 tissue samples was 100% sensitive and 93% specific, having a positive predictive value of 76.9% and negative predictive value of 100%. PCR for M. tuberculosis DNA done on tissue samples was positive for 14 of 19 patients who had a clinical diagnosis of tuberculosis, negative for all six patients with nontuberculous mycobacterial infections, and negative for all 33 patients who had a diagnosis of a disease other than mycobacterial infection. When compared with the clinical diagnosis of tuberculosis, PCR for M. tuberculosis DNA in these patients' tissues was 73.6% sensitive and 100% specific, having a positive predictive value of 100% and negative predictive value of 88.6%. These data indicate that PCR amplification is useful for detecting M. tuberculosis DNA in formalin-fixed tissue specimens, and that it can be used to increase diagnostic accuracy in patients who have perplexing diagnostic problems associated with a granulomatous tissue response.<\/jats:p>","DOI":"10.1164\/ajrccm.158.4.9802034","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T13:47:41Z","timestamp":1365083261000},"page":"1150-1155","source":"Crossref","is-referenced-by-count":59,"title":["Polymerase Chain Reaction to Detect\n                    <i>Mycobacterium tuberculosis<\/i>\n                    in Histologic Specimens"],"prefix":"10.1093","volume":"158","author":[{"given":"NAGESH V.","family":"SALIAN","sequence":"first","affiliation":[{"name":"Departments of Internal Medicine, Microbiology, Pathology, and Anatomy, McClellan Memorial Veterans Affairs Medical Center; and the University of Arkansas for Medical Science, Little Rock, Arkansas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JAMES A.","family":"RISH","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine, Microbiology, Pathology, and Anatomy, McClellan Memorial Veterans Affairs Medical Center; and the University of Arkansas for Medical Science, Little Rock, Arkansas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KATHLEEN D.","family":"EISENACH","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine, Microbiology, Pathology, and Anatomy, McClellan Memorial Veterans Affairs Medical Center; and the University of Arkansas for Medical Science, Little Rock, Arkansas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"DONALD CAVE","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine, Microbiology, Pathology, and Anatomy, McClellan Memorial Veterans Affairs Medical Center; and the University of Arkansas for Medical Science, Little Rock, Arkansas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JOSEPH H.","family":"BATES","sequence":"additional","affiliation":[{"name":"Departments of Internal Medicine, Microbiology, Pathology, and Anatomy, McClellan Memorial Veterans Affairs Medical Center; and the University of Arkansas for Medical Science, Little Rock, Arkansas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1998,10,1]]},"reference":[{"key":"2026032709044212000_B1","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1164\/ajrccm.149.1.8111591","article-title":"Current approaches to the diagnosis of active pulmonary tuberculosis.","volume":"149","author":"Schluger","year":"1994","journal-title":"Am. 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