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Thus interference with the adhesion cascade may provide a useful therapeutic approach in human sepsis. Young healthy men (n = 30) each received a bolus of 4 ng\/kg LPS intravenously to study the effects of endotoxemia on adhesion processes in humans and to identify potential targets for pharmacologic intervention. One third of subjects received pretreatment with 1,000 mg aspirin and 1,000 mg paracetamol to study potential antiinflammatory effects of aspirin or effects of antipyresis. Circulating neutrophils dropped by \u2212 80% at 67 min after LPS, monocytes by \u2212 96% at 90 min, and lymphocytes by \u2212 85% at 240 min. l-selectin expression decreased, particularly on monocytes. Circulating (c)E-selectin levels increased by 820%, von Willebrand factor-Ag (vWF), soluble thrombomodulin, circulating (c)P-selectin, circulating intercellular adhesion molecule-1 (cICAM-1), and circulating vascular cell adhesion molecule-1 (cVCAM-1) by a mean of 65 to 98% (p &amp;lt; 0.001 for all), but cL-selectin by only 15%. Urinary excretion of soluble adhesion molecules was negligible. Aspirin had no influence on the LPS-induced changes of adhesion parameters, but paracetamol blunted the relative increase in vWF while having no effects on the other parameters measured. The consistent, profound, and early upregulation of cE-selectin during endotoxemia indicates that cE-selectin may be a better surrogate marker to monitor the activation status of endothelial cells in systemic inflammation than the other markers measured. Although aspirin did not have any antiinflammatory effects in this model, paracetamol lowered the relative increase in vWF.<\/jats:p>","DOI":"10.1164\/ajrccm.159.3.9805087","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T13:53:03Z","timestamp":1365083583000},"page":"857-863","source":"Crossref","is-referenced-by-count":150,"title":["Regulation of Adhesion Molecules during Human Endotoxemia: No Acute Effects of Aspirin"],"prefix":"10.1093","volume":"159","author":[{"given":"BERND","family":"JILMA","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology, The Adhesion Research Group Elaborating Therapeutics, Clinic for Blood Group Serology and Transfusion Medicine, Department of Transfusion Medicine, University of Vienna, Vienna, Austria; Thrombosis and Vascular Biology Unit, Department of Medicine, University of Birmingham, Birmingham, United Kingdom; Department of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Leipzig, Germany; and Hyphen BioMed, Research Organization, Conflans Sainte Honorine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ANDREW","family":"BLANN","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, The Adhesion Research Group Elaborating Therapeutics, Clinic for Blood Group Serology and Transfusion Medicine, Department of Transfusion Medicine, University of Vienna, Vienna, Austria; Thrombosis and Vascular Biology Unit, Department of Medicine, University of Birmingham, Birmingham, United Kingdom; Department of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Leipzig, Germany; and Hyphen BioMed, Research Organization, Conflans Sainte Honorine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"THOMAS","family":"PERNERSTORFER","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, The Adhesion Research Group Elaborating Therapeutics, Clinic for Blood Group Serology and Transfusion Medicine, Department of Transfusion Medicine, University of Vienna, Vienna, Austria; Thrombosis and Vascular Biology Unit, Department of Medicine, University of Birmingham, Birmingham, United Kingdom; Department of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Leipzig, Germany; and Hyphen BioMed, Research Organization, Conflans Sainte Honorine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PETRA","family":"STOHLAWETZ","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, The Adhesion Research Group Elaborating Therapeutics, Clinic for Blood Group Serology and Transfusion Medicine, Department of Transfusion Medicine, University of Vienna, Vienna, Austria; Thrombosis and Vascular Biology Unit, Department of Medicine, University of Birmingham, Birmingham, United Kingdom; Department of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Leipzig, Germany; and Hyphen BioMed, Research Organization, Conflans Sainte Honorine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HANS-GEORG","family":"EICHLER","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, The Adhesion Research Group Elaborating Therapeutics, Clinic for Blood Group Serology and Transfusion Medicine, Department of Transfusion Medicine, University of Vienna, Vienna, Austria; Thrombosis and Vascular Biology Unit, Department of Medicine, University of Birmingham, Birmingham, United Kingdom; Department of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Leipzig, Germany; and Hyphen BioMed, Research Organization, Conflans Sainte Honorine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BARBARA","family":"VONDROVEC","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, The Adhesion Research Group Elaborating Therapeutics, Clinic for Blood Group Serology and Transfusion Medicine, Department of Transfusion Medicine, University of Vienna, Vienna, Austria; Thrombosis and Vascular Biology Unit, Department of Medicine, University of Birmingham, Birmingham, United Kingdom; Department of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Leipzig, Germany; and Hyphen BioMed, Research Organization, Conflans Sainte Honorine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JEAN","family":"AMIRAL","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, The Adhesion Research Group Elaborating Therapeutics, Clinic for Blood Group Serology and Transfusion Medicine, Department of Transfusion Medicine, University of Vienna, Vienna, Austria; Thrombosis and Vascular Biology Unit, Department of Medicine, University of Birmingham, Birmingham, United Kingdom; Department of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Leipzig, Germany; and Hyphen BioMed, Research Organization, Conflans Sainte Honorine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"VOLKER","family":"RICHTER","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, The Adhesion Research Group Elaborating Therapeutics, Clinic for Blood Group Serology and Transfusion Medicine, Department of Transfusion Medicine, University of Vienna, Vienna, Austria; Thrombosis and Vascular Biology Unit, Department of Medicine, University of Birmingham, Birmingham, United Kingdom; Department of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Leipzig, Germany; and Hyphen BioMed, Research Organization, Conflans Sainte Honorine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"OSWALD F.","family":"WAGNER","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, The Adhesion Research Group Elaborating Therapeutics, Clinic for Blood Group Serology and Transfusion Medicine, Department of Transfusion Medicine, University of Vienna, Vienna, Austria; Thrombosis and Vascular Biology Unit, Department of Medicine, University of Birmingham, Birmingham, United Kingdom; Department of Clinical Chemistry and Pathobiochemistry, University of Leipzig, Leipzig, Germany; and Hyphen BioMed, Research Organization, Conflans Sainte Honorine, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1999,3,1]]},"reference":[{"key":"2026032708580236800_B1","first-page":"327","article-title":"Platelet-leukocyte-endothelial cell interaction on the blood vessel wall.","volume":"34","author":"Celi","year":"1998","journal-title":"Semin. 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