{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T13:51:18Z","timestamp":1774619478839,"version":"3.50.1"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[1999,9,1]],"date-time":"1999-09-01T00:00:00Z","timestamp":936144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1999,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Inhaled nitric oxide ( \u00b7 NO) is used to improve gas exchange and reduce pulmonary vascular resistance (PVR) in patients with the acute respiratory distress syndrome (ARDS). Although controlled studies have shown no survival benefit, some investigators have suggested that inhaled \u00b7 NO may have antiinflammatory properties under these circumstances. In contrast, others have speculated that \u00b7 NO given by inhalation could be cytotoxic, as it combines with superoxide at near diffusion-limited rates to produce the highly reactive oxidant peroxynitrite (ONOO\u2212). We therefore quantified levels of 3-nitrotyrosine, a marker for ONOO\u2212 formation, in bronchoalveolar lavage fluid (BAL) from patients with ARDS receiving inhaled \u00b7 NO, and from patients with comparable lung injury who were not so treated. We also measured levels of 3-chlorotyrosine as an index of neutrophil activation to assess indirectly the effects of inhaled \u00b7 NO on lung inflammation. Patients receiving \u00b7 NO had increased levels of 3-nitrotyrosine (6.76 \u00b1 2.79 versus 0.4 \u00b1 0.15 nmol\/mg of protein, p &amp;lt; 0.05) and 3-chlorotyrosine (7.97 \u00b1 2.74 versus 1.53 \u00b1 1.09 nmol \/ mg of protein, p &amp;lt; 0.05) in BAL protein compared with controls. In patients with ARDS, inhaled \u00b7 NO increases the formation of 3-nitrotyrosine and is accompanied by an increase in levels of 3-chlorotyrosine (a marker of neutrophil activation). The possible long-term consequences of these observations remain to be evaluated.<\/jats:p>","DOI":"10.1164\/ajrccm.160.3.9810048","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T14:02:31Z","timestamp":1365084151000},"page":"1031-1034","source":"Crossref","is-referenced-by-count":63,"title":["Nitration of Proteins in Bronchoalveolar Lavage Fluid from Patients with Acute Respiratory Distress Syndrome Receiving Inhaled Nitric Oxide"],"prefix":"10.1093","volume":"160","author":[{"given":"NICHOLAS J.","family":"LAMB","sequence":"first","affiliation":[{"name":"Unit of Critical Care, Imperial College School of Medicine, Royal Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GREGORY J.","family":"QUINLAN","sequence":"additional","affiliation":[{"name":"Unit of Critical Care, Imperial College School of Medicine, Royal Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SARAH T.","family":"WESTERMAN","sequence":"additional","affiliation":[{"name":"Unit of Critical Care, Imperial College School of Medicine, Royal Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JOHN M. C.","family":"GUTTERIDGE","sequence":"additional","affiliation":[{"name":"Unit of Critical Care, Imperial College School of Medicine, Royal Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"TIMOTHY W.","family":"EVANS","sequence":"additional","affiliation":[{"name":"Unit of Critical Care, Imperial College School of Medicine, Royal Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1999,9,1]]},"reference":[{"key":"2026032708473782400_B1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1097\/00003246-199801000-00011","article-title":"Effects of inhaled nitric oxide in patients with acute respiratory distress syndrome: results of a randomised phase II trial.","volume":"26","author":"Delinger","year":"1998","journal-title":"Crit. 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