{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T13:23:10Z","timestamp":1774617790764,"version":"3.50.1"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2000,5,1]],"date-time":"2000-05-01T00:00:00Z","timestamp":957139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We produced ovalbumin (OVA)-sensitized rats as an animal model of nasal allergy. Intranasal instillation of OVA induced hypertrophic and metaplastic changes of goblet cells in nasal epithelium of OVA- sensitized rats. Intraepithelial mucosubstance in nasal mucosa increased significantly at 24 h after 3 or 7 d of OVA instillation, accompanied by mucosal infiltration of eosinophils. The effects of H1-antagonist (d-chlorpheniramine malate), H2-antagonist (cimetidine), dexamethasone, indomethacin, cysteinyl leukotrienes (cysLTs)-antagonist (ONO1078), and antirat neutrophil antiserum on OVA-induced changes were examined. Mucus production was significantly inhibited by dexamethasone, and ONO1078, whereas eosinophil infiltration was significantly inhibited by H1-antagonist, dexamethasone, and anti-rat neutrophil antiserum. These results indicate that cysLTs (LTs C4, D4, and E4) may play an important role in antigen-induced mucus production, and that eosinophil infiltration does not relate to mucus production. Intranasal instillation of lipopolysaccharide (LPS) also induced intraepithelial mucus production, and it was significantly inhibited by dexamethasone, indomethacin, and antirat neutrophil antiserum; however, cysLTs antagonist had no effect on LPS-induced change. These results indicate that neutrophil and cyclooxygenase products are important in LPS-induced mucus production, and there are different mechanisms of mucus production between allergic inflammation and LPS stimulation.<\/jats:p>","DOI":"10.1164\/ajrccm.161.5.9908101","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T14:16:55Z","timestamp":1365085015000},"page":"1648-1654","source":"Crossref","is-referenced-by-count":39,"title":["A Mechanism of Antigen-induced Mucus Production in Nasal Epithelium of Sensitized Rats: A Comparison with Lipopolysaccharide-induced Mucus Production"],"prefix":"10.1093","volume":"161","author":[{"given":"TAKESHI","family":"SHIMIZU","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology, Mie University School of Medicine, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HITOMI","family":"HIRANO","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Mie University School of Medicine, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YUICHI","family":"MAJIMA","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Mie University School of Medicine, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YASUO","family":"SAKAKURA","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Mie University School of Medicine, Mie, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2000,5,1]]},"reference":[{"key":"2026032708394698100_B1","first-page":"90","article-title":"Effects of an ambient level of ozone on primate nasal epithelial mucosubstances.","volume":"127","author":"Harkema","year":"1987","journal-title":"Am. J. Pathol."},{"key":"2026032708394698100_B2","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1165\/ajrcmb\/9.3.264","article-title":"Effect of nitrogen dioxide on human nasal epithelium.","volume":"9","author":"Carson","year":"1993","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026032708394698100_B3","first-page":"635","article-title":"Quantitative study of secretory cell metaplasia induced by human neutrophil elastase in the large bronchi of hamsters.","volume":"105","author":"Breuer","year":"1985","journal-title":"J. Lab. Clin. Med."},{"key":"2026032708394698100_B4","doi-asserted-by":"crossref","first-page":"227","DOI":"10.3109\/01902148309046062","article-title":"Changes in secretory cells of hamster tracheal epithelium in response to acute sublethal injury: a quantitative study.","volume":"4","author":"McDowell","year":"1983","journal-title":"Exp. Lung Res."},{"key":"2026032708394698100_B5","doi-asserted-by":"crossref","first-page":"853","DOI":"10.3109\/01902148809087849","article-title":"Dexamethasone reduces rat tracheal goblet cell hyperplasia produced by human neutrophil products.","volume":"14","author":"Lundgren","year":"1988","journal-title":"Exp. Lung Res."},{"key":"2026032708394698100_B6","doi-asserted-by":"crossref","first-page":"710","DOI":"10.3109\/00016489209137464","article-title":"Newcastle disease viral infection in chicken nasal turbinate and maxillary sinus.","volume":"112","author":"Ukai","year":"1992","journal-title":"Acta Otolaryngol."},{"key":"2026032708394698100_B7","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1164\/ajrccm.153.4.8616574","article-title":"Hypertrophic and metaplastic changes of goblet cells in rat nasal epithelium induced by endotoxin.","volume":"153","author":"Shimizu","year":"1996","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"2026032708394698100_B8","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1165\/ajrcmb.14.5.8624247","article-title":"Lung inflammation and epithelial changes in a murine model of atopic asthma.","volume":"4","author":"Blyth","year":"1996","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026032708394698100_B9","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1164\/ajrccm.158.4.9801041","article-title":"Goblet cell degradation after antigen challenge in sensitized guinea pig.","volume":"158","author":"Agusti","year":"1998","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"2026032708394698100_B10","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1165\/ajrcmb.13.2.7626283","article-title":"Effect of dexamethasone on antigen-induced high molecular weight glycoconjugate secretion in allergic guinea pigs.","volume":"13","author":"Sovoie","year":"1995","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026032708394698100_B11","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1165\/ajrcmb.16.4.9115759","article-title":"A novel role for murine IL-4 in vitro: induction of MUC5AC gene expression and mucin hypersecretion.","volume":"16","author":"Temann","year":"1997","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026032708394698100_B12","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1165\/ajrcmb.20.5.3446","article-title":"Mucous-cell metaplasia and inflammatory-cell recruitment are dissociated in allergic mice after antibody- and drug-dependent cell depletion in a murine model of asthma.","volume":"20","author":"Haile","year":"1999","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026032708394698100_B13","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1159\/000237361","article-title":"In vivo effects of monoclonal antibody against ICAM-1 and LFA-1 on antigen-induced nasal symptoms and eosinophilia in sensitized rats.","volume":"111","author":"Asakura","year":"1996","journal-title":"Int. Arch. Allergy Immunol."},{"key":"2026032708394698100_B14","first-page":"605","article-title":"In vivo effects of transient neutrophil influx on nasal respiratory epithelial mucosubstances.","volume":"130","author":"Harkema","year":"1988","journal-title":"Am. J. Pathol."},{"key":"2026032708394698100_B15","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0021-5198(19)32412-6","article-title":"In vivo pharmacologic profile of ONO-1078: a potent, selective and orally active peptide leukotriene (LT) antagonist.","volume":"60","author":"Nakagawa","year":"1992","journal-title":"Jpn. J. Pharmacol."},{"key":"2026032708394698100_B16","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1165\/ajrcmb.19.1.2930","article-title":"Induction, duration, and resolution of airway goblet cell hyperplasia in a murine model of atopic asthma: effect of concurrent infection with respiratory syncytial virus and response to dexamethasone.","volume":"19","author":"Blyth","year":"1998","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026032708394698100_B17","doi-asserted-by":"crossref","first-page":"S763","DOI":"10.1016\/S0091-6749(97)70125-8","article-title":"Pathogenesis of allergic rhinitis.","volume":"99","author":"Baraniuk","year":"1997","journal-title":"J. Allergy Clin. Immunol."},{"key":"2026032708394698100_B18","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1016\/0091-6749(92)90155-U","article-title":"Mediators of allergic rhinitis.","volume":"90","author":"White","year":"1992","journal-title":"J. Allergy Clin. Immunol."},{"key":"2026032708394698100_B19","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1164\/ajrccm\/139.3.791","article-title":"The pathophysiology of rhinitis: II. Assessment of the sources of protein in histamine-induced nasal secretions.","volume":"139","author":"Raphael","year":"1989","journal-title":"Am. Rev. Respir. Dis."},{"key":"2026032708394698100_B20","first-page":"L590","article-title":"Effects of histamine on guinea pig nasal mucosal secretion.","volume":"262","author":"Gawin","year":"1992","journal-title":"Am. J. Physiol."},{"key":"2026032708394698100_B21","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1164\/ajrccm.154.1.8680685","article-title":"Effect of oxitropium bromide on histamine-induced airway goblet cell secretion.","volume":"154","author":"Takeyama","year":"1996","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"2026032708394698100_B22","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/sj.bjp.0701886","article-title":"Effects of the cysteinyl luekotriene receptor antagonists planlukast and zafirlukast on tracheal mucous secretion in ovalubumin-sensitized guinea pigs in vitro.","volume":"124","author":"Liu","year":"1998","journal-title":"Br. J. Pharmacol."},{"key":"2026032708394698100_B23","first-page":"413","article-title":"Inflammatory cells and mediator release during ragweed challenge: correlation between histamine content in nasal secretions and appearance of inflammatory cells.","volume":"68","author":"Georgitis","year":"1992","journal-title":"Ann. Allergy"},{"key":"2026032708394698100_B24","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1056\/NEJM198406213102502","article-title":"Peptide leukotriene release after antigen challenge in patients sensitive to ragweed.","volume":"310","author":"Creticos","year":"1984","journal-title":"N. Engl. J. Med."},{"key":"2026032708394698100_B25","first-page":"537","article-title":"The role of leukotriene D4 in allergic rhinitis.","volume":"60","author":"Okuda","year":"1988","journal-title":"Ann. Allergy"},{"key":"2026032708394698100_B26","doi-asserted-by":"crossref","first-page":"1734","DOI":"10.1164\/ajrccm.151.6.7767514","article-title":"The leukotriene D4-receptor antagonist, ICI 204219, relieves symptoms of acute seasonal allergic rhinitis.","volume":"151","author":"Donnelly","year":"1995","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"2026032708394698100_B27"}],"container-title":["American Journal of Respiratory and Critical Care Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/161\/5\/1648\/67425043\/ajrccm_161_5_1648.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/161\/5\/1648\/67425043\/ajrccm_161_5_1648.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T12:39:57Z","timestamp":1774615197000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/article\/161\/5\/1648\/8528657"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5,1]]},"references-count":27,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2000,5,1]]},"published-print":{"date-parts":[[2000,5,1]]}},"URL":"https:\/\/doi.org\/10.1164\/ajrccm.161.5.9908101","relation":{},"ISSN":["1073-449X","1535-4970"],"issn-type":[{"value":"1073-449X","type":"print"},{"value":"1535-4970","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2000,5]]},"published":{"date-parts":[[2000,5,1]]}}}