{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:13:26Z","timestamp":1791242006286,"version":"4.1.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2001,9,1]],"date-time":"2001-09-01T00:00:00Z","timestamp":999302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Using a BLAST-searching approach, we identified a mouse expressed sequence tag (EST) clone (AA038672) showing great similarity to the 3\u2032 end of the human MUC5B gene. The clone was named \u201c3pmmuc5b-1\u201d after complete nucleotide sequencing (Genbank Accession, AF369933). A subsequent search of the mouse genome database with the 3pmmuc5b-1 sequence identified two overlapping genomic clones (AC020817 and AC020794) that contained the sequence of both 3pmmuc5b-1 and the mouse Muc5ac gene. Like their human homologs, the genomic order of the mouse Muc genes is 5\u2032 -Muc5ac-Muc5b-3\u2032. These results suggest that the newly identified EST clone, 3pmmuc5b-1, is part of the 3\u2032 portion of the mouse Muc5b gene. In situ hybridization demonstrated that this putative mouse Muc5b message was expressed in a restricted manner in the sublingual gland region of the tongue and the submucosal gland region of the mouse trachea in a normal animal. However, the gene expression was greatly enhanced in airway surface epithelium and the submucosal gland region in ovalbumin-induced asthmatic mice. These results were consistent with previous studies of human airway tissues. We therefore conclude that this newly cloned mouse Muc5b gene could be used as a marker for studying aberrant mucin gene expression in mouse models of various airway diseases.<\/jats:p>","DOI":"10.1164\/ajrccm.164.6.2012114","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T14:50:15Z","timestamp":1365087015000},"page":"1059-1066","source":"Crossref","is-referenced-by-count":55,"title":["<i>In Silico<\/i>\n                    Cloning of Mouse\n                    <i>Muc5b<\/i>\n                    Gene and Upregulation of its Expression in Mouse Asthma Model"],"prefix":"10.1093","volume":"164","author":[{"given":"YIN","family":"CHEN","sequence":"first","affiliation":[{"name":"Center for Comparative Respiratory Biology and Medicine, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YU HUA","family":"ZHAO","sequence":"additional","affiliation":[{"name":"Center for Comparative Respiratory Biology and Medicine, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"REEN","family":"WU","sequence":"additional","affiliation":[{"name":"Center for Comparative Respiratory Biology and Medicine, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,9,15]]},"reference":[{"key":"2026032708054225800_B1","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1378\/chest.101.4.916","article-title":"Marked goblet cell hyperplasia with mucus accumulation in the airways of patients who died of severe acute asthma attack.","volume":"101","author":"Aikawa","year":"1992","journal-title":"Chest"},{"key":"2026032708054225800_B2","first-page":"612","article-title":"Human respiratory mucus.","volume":"134","author":"Kaliner","year":"1986","journal-title":"Am Rev Respir Dis"},{"key":"2026032708054225800_B3","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1095\/biolreprod51.4.685","article-title":"Complementary deoxyribonucleic acid cloning and molecular characterization of an estrogen-dependent human oviductal glycoprotein.","volume":"51","author":"Arias","year":"1994","journal-title":"Biol Reprod"},{"key":"2026032708054225800_B4","first-page":"4083","article-title":"Two novel mucin genes down-regulated in colorectal cancer identified by differential display.","volume":"59","author":"Williams","year":"1999","journal-title":"Cancer Res"},{"key":"2026032708054225800_B5"},{"key":"2026032708054225800_B6","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1093\/oxfordjournals.molbev.a026400","article-title":"Evolution of the large secreted gel-forming mucins.","volume":"17","author":"Desseyn","year":"2000","journal-title":"Mol Biol Evol"},{"key":"2026032708054225800_B7","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1006\/geno.1996.0637","article-title":"Human mucin genes assigned to 11p15.5: identification and organization of a cluster of genes.","volume":"38","author":"Pigny","year":"1996","journal-title":"Genomics"},{"key":"2026032708054225800_B8","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1042\/bj3160967","article-title":"Respiratory mucins: identification of core proteins and glycoforms.","volume":"316","author":"Thornton","year":"1996","journal-title":"Biochem J"},{"key":"2026032708054225800_B9","doi-asserted-by":"crossref","first-page":"9561","DOI":"10.1074\/jbc.272.14.9561","article-title":"Identification of two glycoforms of the MUC5B mucin in human respiratory mucus. 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