{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T12:58:22Z","timestamp":1774616302298,"version":"3.50.1"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2001,10,1]],"date-time":"2001-10-01T00:00:00Z","timestamp":1001894400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A novel 85 kD glycoprotein (gp85) is a marker of the avesicular zone, a thin part of pulmonary endothelial cells separating alveolar and vascular compartments and lacking vesicles. This report presents the first evaluation whether mAb 30B3, a monoclonal antibody to gp85, can be used for targeting of drugs to the surface of lung endothelium. 125I-mAb 30B3 accumulated in isolated perfused lungs (IPL) (22.8 \u00b1 1.1 versus 0.5 \u00b1 0.1 %ID\/g for 125I-IgG) and accumulated preferentially in the lungs after intravenous or intraarterial injection (10.9 \u00b1 0.7 and 11.0 \u00b1 1.5 versus 0.9 \u00b1 0.2 %ID\/g for 125I-IgG). 125I-mAb 30B3 uptake in IPL was rapid (T1\/2 15 min), saturable (Bmax appr. 105 molecules\/cell), specific (inhibited by nonlabeled mAb 30B3) and temperature independent (26.3 \u00b1 2.1 versus 22.8 \u00b1 1.1 %ID\/g at 6\u00b0 C versus 37\u00b0 C). Biotinylated mAb 30B3 permitted subsequent accumulation of perfused avidin derivative in IPL. Because these data indicated that mAb 30B3 binds to an accessible, poorly internalizable antigen in the lung, we conjugated mAb 30B3 with a plasminogen activator, 125I-tPA. After intravenous injection in rats, lung-to-blood ratio was 8.4 \u00b1 0.9 for mAb 30B3\/125I-tPA versus 0.4 \u00b1 0.1 for IgG\/125I-tPA, indicating that mAb 30B3 may deliver drugs, which was supposed to exert therapeutic action in the vascular lumen (e.g., antithrombotic proteins), to the surface of pulmonary endothelium.<\/jats:p>","DOI":"10.1164\/ajrccm.164.7.2010076","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T14:54:06Z","timestamp":1365087246000},"page":"1295-1302","source":"Crossref","is-referenced-by-count":29,"title":["Vascular Immunotargeting to Endothelial Surface in a Specific Macrodomain in Alveolar Capillaries"],"prefix":"10.1093","volume":"164","author":[{"given":"JUAN CARLOS","family":"MURCIANO","sequence":"first","affiliation":[{"name":"Institute for Environmental Medicine and Department of Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania; Department of Pathology and Cell Biology, University of Montreal, Montreal, Quebec, Canada; Department of Anesthesiology, University of Illinois at Chicago, Chicago, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"WIN HARSHAW","sequence":"additional","affiliation":[{"name":"Institute for Environmental Medicine and Department of Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania; Department of Pathology and Cell Biology, University of Montreal, Montreal, Quebec, Canada; Department of Anesthesiology, University of Illinois at Chicago, Chicago, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LUCIAN","family":"GHITESCU","sequence":"additional","affiliation":[{"name":"Institute for Environmental Medicine and Department of Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania; Department of Pathology and Cell Biology, University of Montreal, Montreal, Quebec, Canada; Department of Anesthesiology, University of Illinois at Chicago, Chicago, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SERGEI M.","family":"DANILOV","sequence":"additional","affiliation":[{"name":"Institute for Environmental Medicine and Department of Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania; Department of Pathology and Cell Biology, University of Montreal, Montreal, Quebec, Canada; Department of Anesthesiology, University of Illinois at Chicago, Chicago, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"VLADIMIR R.","family":"MUZYKANTOV","sequence":"additional","affiliation":[{"name":"Institute for Environmental Medicine and Department of Pharmacology, University of Pennsylvania, Philadelphia, Pennsylvania; Department of Pathology and Cell Biology, University of Montreal, Montreal, Quebec, Canada; Department of Anesthesiology, University of Illinois at Chicago, Chicago, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,10,1]]},"reference":[{"key":"2026032708082834000_B1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/0022-2828(89)90853-5","article-title":"Monoclonal antibodies to ACE: a powerful tool for lung and vessel studies.","volume":"21","author":"Danilov","year":"1989","journal-title":"J Mol Cell Cardiol"},{"key":"2026032708082834000_B2","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0928-4680(98)00006-6","article-title":"Immunotargeting of drugs to the pulmonary vascular endothelium as a therapeutic strategy.","volume":"5","author":"Muzykantov","year":"1998","journal-title":"Pathophysiology"},{"key":"2026032708082834000_B3","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1056\/NEJM199808133390711","article-title":"Vascular targeting as a strategy for cancer therapy.","volume":"339","author":"Schnitzer","year":"1998","journal-title":"N Engl J Med"},{"key":"2026032708082834000_B4","first-page":"118","article-title":"Lung is a target organ for monoclonal antibody to ACE.","volume":"64","author":"Danilov","year":"1991","journal-title":"Lab Invest"},{"key":"2026032708082834000_B5","doi-asserted-by":"crossref","first-page":"1464","DOI":"10.1164\/ajrccm\/139.6.1464","article-title":"In vivo administration of glucose oxidase conjugated with monoclonal antibody to ACE: targeting into the lung.","volume":"136","author":"Muzykantov","year":"1989","journal-title":"Am Rev Respir Dis"},{"key":"2026032708082834000_B6","first-page":"193","article-title":"Rat monoclonal antibody distribution in mice: an epitope inside the lung vascular space mediates very efficient localization.","volume":"17","author":"Kennel","year":"1990","journal-title":"Nucl Med Biol"},{"key":"2026032708082834000_B7","first-page":"L504","article-title":"Targeted gene delivery to pulmonary endothelium by anti-PECAM antibody.","volume":"278","author":"Li","year":"2000","journal-title":"Am J Physiol"},{"key":"2026032708082834000_B8","doi-asserted-by":"crossref","first-page":"2379","DOI":"10.1073\/pnas.96.5.2379","article-title":"Streptavidin facilitates internalization and pulmonary uptake of an anti-endothelial cell antibody (PECAM-1): a strategy for vascular immunotargeting of drugs.","volume":"96","author":"Muzykantov","year":"1999","journal-title":"Proc Natl Acad Sci USA"},{"key":"2026032708082834000_B9","first-page":"L806","article-title":"Immunotargeting of catalase to ACE or ICAM-1 protects perfused rat lungs against oxidative stress.","volume":"27519","author":"Atochina","year":"1998","journal-title":"Am J Physiol"},{"key":"2026032708082834000_B10","doi-asserted-by":"crossref","first-page":"6773","DOI":"10.1073\/pnas.85.18.6773","article-title":"Albumin interacts specifically with a 60-kDa microvascular endothelial glycoprotein.","volume":"85","author":"Schnitzer","year":"1988","journal-title":"Proc Natl Acad Sci USA"},{"key":"2026032708082834000_B11","first-page":"L1335","article-title":"Lung uptake of antibodies to endothelial antigens: key determinants of vascular immunotargeting.","volume":"280","author":"Danilov","year":"2001","journal-title":"Am J Physiol"},{"key":"2026032708082834000_B12"},{"key":"2026032708082834000_B13","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s002329900474","article-title":"Endocytosis and exocytosis events regulate vesicle traffic in endothelial cells.","volume":"167","author":"Niles","year":"1999","journal-title":"J Membrane Biol"},{"key":"2026032708082834000_B14","first-page":"L704","article-title":"Endothelial cells internalize monoclonal antibody to ACE.","volume":"270","author":"Muzykantov","year":"1996","journal-title":"Am J Physiol"},{"key":"2026032708082834000_B15","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/0026-2862(83)90045-6","article-title":"Differential distribution of the cell surface charge on the alveolar capillary unit. 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