{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:21:00Z","timestamp":1790508060673,"version":"4.1.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Denitrifying bacteria metabolize nitrogen oxides through assimilatory and dissimilatory pathways. These redox reactions may affect lung physiology. We hypothesized that airway colonization with denitrifying bacteria could alter nitrogen balance in the cystic fibrosis (CF) airway. We measured airway nitrogen redox species before and after antimicrobial therapy for Pseudomonas aeruginosa in patients with CF. We also studied ammonium (NH4+) and nitric oxide (NO) metabolism in clinical strains of P. aeruginosa in vitro and in CF sputum ex vivo. Ammonium concentrations in both sputum and tracheal aspirates decreased with therapy. Nitric oxide reductase (NOR) was present in clinical strains of P. aeruginosa, which both produced NH4+ and consumed NO. Further, NO consumption by CF sputum was inhibited by tobramycin ex vivo. We conclude that treatment of pseudomonal lung infections is associated with decreased NH4+ concentrations in the CF airways. In epithelial cells, NH4+ inhibits chloride transport, and nitrogen oxides inhibit amiloride-sensitive sodium transport and augment chloride transport. We speculate that normalization of airway nitrogen redox balance could contribute to the beneficial effects of antipseudomonal therapy on lung function in CF.<\/jats:p>","DOI":"10.1164\/ajrccm.165.3.2106006","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T15:00:57Z","timestamp":1365087657000},"page":"387-390","source":"Crossref","is-referenced-by-count":73,"title":["Nitrogen Redox Balance in the Cystic Fibrosis Airway: Effects of Antipseudomonal Therapy"],"prefix":"10.1093","volume":"165","author":[{"given":"Benjamin","family":"Gaston","sequence":"first","affiliation":[{"name":"Departments of Pediatric Pulmonary Medicine and Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia; and Department of Pediatric Pulmonary Medicine, Children's Hospital, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Ratjen","sequence":"additional","affiliation":[{"name":"Departments of Pediatric Pulmonary Medicine and Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia; and Department of Pediatric Pulmonary Medicine, Children's Hospital, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Vaughan","sequence":"additional","affiliation":[{"name":"Departments of Pediatric Pulmonary Medicine and Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia; and Department of Pediatric Pulmonary Medicine, Children's Hospital, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil R.","family":"Malhotra","sequence":"additional","affiliation":[{"name":"Departments of Pediatric Pulmonary Medicine and Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia; and Department of Pediatric Pulmonary Medicine, Children's Hospital, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert G.","family":"Canady","sequence":"additional","affiliation":[{"name":"Departments of Pediatric Pulmonary Medicine and Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia; and Department of Pediatric Pulmonary Medicine, Children's Hospital, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley H.","family":"Snyder","sequence":"additional","affiliation":[{"name":"Departments of Pediatric Pulmonary Medicine and Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia; and Department of Pediatric Pulmonary Medicine, Children's Hospital, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John F.","family":"Hunt","sequence":"additional","affiliation":[{"name":"Departments of Pediatric Pulmonary Medicine and Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia; and Department of Pediatric Pulmonary Medicine, Children's Hospital, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Gaertig","sequence":"additional","affiliation":[{"name":"Departments of Pediatric Pulmonary Medicine and Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia; and Department of Pediatric Pulmonary Medicine, Children's Hospital, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna B.","family":"Goldberg","sequence":"additional","affiliation":[{"name":"Departments of Pediatric Pulmonary Medicine and Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia; and Department of Pediatric Pulmonary Medicine, Children's Hospital, Essen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,2,1]]},"reference":[{"key":"2026031623120945400_B1","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1002\/(SICI)1096-9896(199803)184:3<323::AID-PATH2>3.0.CO;2-2","article-title":"Lack of inducible nitric oxide synthase in bronchial epithelium: a possible mechanism of susceptibility to infection in cystic fibrosis.","volume":"184","author":"Meng","year":"1998","journal-title":"J Pathol"},{"key":"2026031623120945400_B2","doi-asserted-by":"crossref","first-page":"2172","DOI":"10.1164\/ajrccm.162.6.2003106","article-title":"Airway NO levels in CF patients are related to a polymorphism in the neuronal NO synthase (NOSI) gene.","volume":"162","author":"Grasemann","year":"2000","journal-title":"Am J Respir Crit Care Med"},{"key":"2026031623120945400_B3","first-page":"L1120","article-title":"Superoxide released from neutrophils causes a reduction in nitric oxide gas.","volume":"275","author":"Jones","year":"1998","journal-title":"Am J Physiol"},{"key":"2026031623120945400_B4","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.1183\/09031936.96.09122537","article-title":"Airway nitric oxide in asthmatic children and patients with cystic fibrosis.","volume":"9","author":"Dotsch","year":"1996","journal-title":"Eur Respir J"},{"key":"2026031623120945400_B5"},{"key":"2026031623120945400_B6","first-page":"533","article-title":"Cell biology and molecular basis of denitrification.","volume":"61","author":"Zumft","year":"1997","journal-title":"Microbiol Molec Biol Rev"},{"key":"2026031623120945400_B7"},{"key":"2026031623120945400_B8","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/S0022-3476(97)70204-X","article-title":"Expired nitric oxide as a marker for childhood asthma.","volume":"130","author":"Nelson","year":"1997","journal-title":"J Pediatr"},{"key":"2026031623120945400_B9","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1164\/ajrccm.153.5.8630594","article-title":"Elevated nitric oxide concentrations in isolated lower airway gas of asthmatic subjects.","volume":"153","author":"Massaro","year":"1996","journal-title":"Am J Respir Crit Care Med"},{"key":"2026031623120945400_B10","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1164\/ajrccm.159.1.9803034","article-title":"Vital capacity reservoir and online measurement of childhood nitrosopnea are linearly related. 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