{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:39:20Z","timestamp":1791175160648,"version":"4.1.0"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2003,6,1]],"date-time":"2003-06-01T00:00:00Z","timestamp":1054425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The virulence of Pseudomonas aeruginosa is partly controlled by the las quorum-sensing system. A rat model of acute pneumonia was used to investigate the pathophysiological impact of this system by comparing the virulence of the wild-type virulent laboratory strain PAO1 with that of its lasR-deleted mutant PAOR. In comparison with PAO1, PAOR was avirulent after an instillation of 106 cfu (mortality rates, 72 versus 0%, respectively; p &amp;lt; 0.0001). A ten-fold higher inoculum slightly increased the mortality rate induced by PAOR (25%), which remained lower than that induced by PAO1 (75%, p = 0.0001). In addition, with both inocula lung and bronchoalveolar lavage bacterial counts were significantly lower in rats infected with PAOR than with PAO1 (p \u2264 0.01). Histopathological analysis showed that PAO1 induced a drastic vascular congestion and neutrophil infiltration of the lungs, whereas lung injury in rats infected with PAOR was mild with predominantly macrophage infiltration. This study adds evidence that the quorum-sensing system has an important role in the pathophysiology of P. aeruginosa pulmonary infection.<\/jats:p>","DOI":"10.1164\/rccm.200207-736bc","type":"journal-article","created":{"date-parts":[[2003,2,3]],"date-time":"2003-02-03T20:22:39Z","timestamp":1044303759000},"page":"1478-1482","source":"Crossref","is-referenced-by-count":72,"title":["Role of the Quorum-sensing System in Experimental Pneumonia due to\n                    <i>Pseudomonas aeruginosa<\/i>\n                    in Rats"],"prefix":"10.1093","volume":"167","author":[{"given":"Philippe","family":"Lesprit","sequence":"first","affiliation":[{"name":"INSERM EMI-U 9933, and Service d'Anatomie Pathologique, H\u00f4pital Bichat, Paris, France; D\u00e9partement de Sant\u00e9 Publique, H\u00f4pital Henri Mondor, Cr\u00e9teil; and Laboratoire d'Ing\u00e9nierie des Syst\u00e8mes Macromol\u00e9culaires, Centre National de la Recherche Scientifique, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Faurisson","sequence":"additional","affiliation":[{"name":"INSERM EMI-U 9933, and Service d'Anatomie Pathologique, H\u00f4pital Bichat, Paris, France; D\u00e9partement de Sant\u00e9 Publique, H\u00f4pital Henri Mondor, Cr\u00e9teil; and Laboratoire d'Ing\u00e9nierie des Syst\u00e8mes Macromol\u00e9culaires, Centre National de la Recherche Scientifique, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Join-Lambert","sequence":"additional","affiliation":[{"name":"INSERM EMI-U 9933, and Service d'Anatomie Pathologique, H\u00f4pital Bichat, Paris, France; D\u00e9partement de Sant\u00e9 Publique, H\u00f4pital Henri Mondor, Cr\u00e9teil; and Laboratoire d'Ing\u00e9nierie des Syst\u00e8mes Macromol\u00e9culaires, Centre National de la Recherche Scientifique, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Roudot-Thoraval","sequence":"additional","affiliation":[{"name":"INSERM EMI-U 9933, and Service d'Anatomie Pathologique, H\u00f4pital Bichat, Paris, France; D\u00e9partement de Sant\u00e9 Publique, H\u00f4pital Henri Mondor, Cr\u00e9teil; 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