{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T14:19:24Z","timestamp":1791123564884,"version":"4.1.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2003,9,1]],"date-time":"2003-09-01T00:00:00Z","timestamp":1062374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>To examine the in vivo effects of macrolide antibiotics on mucus hypersecretion, we induced hypertrophic and metaplastic changes of goblet cells in rat nasal epithelium by intranasal instillation of ovalbumin (OVA) in OVA-sensitized rats and by intranasal LPS instillation. Oral administration of clarithromycin (CAM) (5\u201310 mg\/kg) significantly inhibited OVA- and LPS-induced mucus production and neutrophil infiltration, whereas josamycin and ampicillin showed no effect. In vitro effects of macrolide antibiotics on airway epithelial cells were examined using NCI-H292 cells and human nasal epithelial cells cultured in air\u2013liquid interface. Mucus secretion was evaluated by ELISA using anti-mucin monoclonal antibodies (anti-MUC5AC and HCS18). CAM and erythromycin significantly inhibited spontaneous and tumor necrosis factor\u2013\u03b1 (20 ng\/ml)\u2013induced mucus secretion from NCI-H292 cells at 10\u22126 to 10\u22127 M and from human nasal epithelial cells at 10\u22124 to 10\u22125 M. MUC5AC messenger RNA expression was also significantly inhibited. These results indicate that the 14-member macrolide antibiotics, CAM and erythromycin, exert direct inhibitory effects on mucus secretion from airway epithelial cells and that they may be useful for the treatment of mucus hypersecretion caused by allergic inflammation and LPS stimulation.<\/jats:p>","DOI":"10.1164\/rccm.200212-1437oc","type":"journal-article","created":{"date-parts":[[2003,6,30]],"date-time":"2003-06-30T20:24:52Z","timestamp":1057004692000},"page":"581-587","source":"Crossref","is-referenced-by-count":132,"title":["<i>In Vivo<\/i>\n                    and\n                    <i>In Vitro<\/i>\n                    Effects of Macrolide Antibiotics on Mucus Secretion in Airway Epithelial Cells"],"prefix":"10.1093","volume":"168","author":[{"given":"Takeshi","family":"Shimizu","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology and Third Department of Internal Medicine, Mie University School of Medicine, Edobashi, Tsu, Mie, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shino","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology and Third Department of Internal Medicine, Mie University School of Medicine, Edobashi, Tsu, Mie, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reiko","family":"Hattori","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology and Third Department of Internal Medicine, Mie University School of Medicine, Edobashi, Tsu, Mie, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban C.","family":"Gabazza","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology and Third Department of Internal Medicine, Mie University School of Medicine, Edobashi, Tsu, Mie, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichi","family":"Majima","sequence":"additional","affiliation":[{"name":"Department of 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