{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:58:07Z","timestamp":1789181887463,"version":"build-2803163510"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>We previously showed an important role of natural killer T cells (NKT) in skewing the adaptive T cell immunity to Chlamydia pneumoniae (Cpn), an intracellular bacterial lung infection, but the mechanism remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To investigate the underlying mechanism by which NKT modulate T cell responses in chlamydial pneumonia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We examined the effect of NKT activation in modulating DC function, especially in generating protective immunity against Cpn infection using combination of NKT knockout (KO) mice and specific NKT activation approaches.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Measurements and Main Results<\/jats:title>\n                    <jats:p>We found that NKT activation in vivo after Cpn infection induces phenotypic and functional changes in dendritic cells (DC). DC from NKT-deficient mice showed reduced CD40 expression and IL-12 production, whereas enhancing NKT activation using \u03b1-GalCer increased CD40 expression and IL-12 production. Co-culture of DC with NKT enhanced bioactive IL-12p70 production by DC in a CD40L-, IFN-\u03b3\u2013, and cell\u2013cell contact\u2013dependent manner. Further, co-culture of T cells with DC isolated from infected wild-type (WT) and NKT-deficient mice induced type-1 and type-2 responses, respectively, while DC from \u03b1-GalCer\u2013treated, infected mice led to enhanced type-1 responses. Moreover, upon adoptive transfer, DC from infected WT mice induced strong type-1 immunity, whereas those from knockout mice induced type-2 responses and increased disease severity upon challenge infection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our results provide direct evidence of the critical role of NKT activation in the functional modulation of DC for the development of protective immunity in a clinically relevant respiratory infection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1164\/rccm.200804-517oc","type":"journal-article","created":{"date-parts":[[2008,7,2]],"date-time":"2008-07-02T21:09:09Z","timestamp":1215032949000},"page":"745-756","source":"Crossref","is-referenced-by-count":46,"title":["Natural Killer T Cells Are Critical for Dendritic Cells to Induce Immunity in Chlamydial Pneumonia"],"prefix":"10.1093","volume":"178","author":[{"given":"Antony","family":"George Joyee","sequence":"first","affiliation":[{"name":"Laboratory for Infection and Immunity, Departments of Medical Microbiology and Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyu","family":"Qiu","sequence":"additional","affiliation":[{"name":"Laboratory for Infection and Immunity, Departments of Medical Microbiology and Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijun","family":"Fan","sequence":"additional","affiliation":[{"name":"Laboratory for Infection and Immunity, Departments of Medical Microbiology and Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhe","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory for Infection and Immunity, Departments of Medical Microbiology and Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory for Infection and Immunity, Departments of Medical Microbiology and Immunology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2008,10,1]]},"reference":[{"key":"2026032102293334800_BIB1","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/nri1309","volume":"4","year":"2004","journal-title":"Nat Rev Immunol"},{"key":"2026032102293334800_BIB2","first-page":"754","volume":"6","year":"2005","journal-title":"Nat Immunol"},{"key":"2026032102293334800_BIB3","doi-asserted-by":"crossref","first-page":"5447","DOI":"10.1128\/IAI.71.10.5447-5455.2003","volume":"71","year":"2003","journal-title":"Infect Immun"},{"key":"2026032102293334800_BIB4","doi-asserted-by":"crossref","first-page":"2691","DOI":"10.2337\/diabetes.50.12.2691","volume":"50","year":"2001","journal-title":"Diabetes"},{"key":"2026032102293334800_BIB5","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1084\/jem.191.4.661","volume":"191","year":"2000","journal-title":"J Exp Med"},{"key":"2026032102293334800_BIB6","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1089\/152091502760098465","volume":"4","year":"2002","journal-title":"Diabetes Technol Ther"},{"key":"2026032102293334800_BIB7","doi-asserted-by":"crossref","first-page":"1521","DOI":"10.1084\/jem.188.8.1521","volume":"188","year":"1998","journal-title":"J Exp Med"},{"key":"2026032102293334800_BIB8","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/S0198-8859(98)00100-1","volume":"60","year":"1999","journal-title":"Hum Immunol"},{"key":"2026032102293334800_BIB9","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1146\/annurev.immunol.21.120601.141057","volume":"21","year":"2003","journal-title":"Annu Rev Immunol"},{"key":"2026032102293334800_BIB10","doi-asserted-by":"crossref","first-page":"4647","DOI":"10.4049\/jimmunol.163.9.4647","volume":"163","year":"1999","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB11","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1038\/32588","volume":"392","year":"1998","journal-title":"Nature"},{"key":"2026032102293334800_BIB12","doi-asserted-by":"crossref","first-page":"957","DOI":"10.4049\/jimmunol.167.2.957","volume":"167","year":"2001","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB13","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1084\/jem.20050810","volume":"202","year":"2005","journal-title":"J Exp Med"},{"key":"2026032102293334800_BIB14","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1084\/jem.20030324","volume":"198","year":"2003","journal-title":"J Exp Med"},{"key":"2026032102293334800_BIB15","doi-asserted-by":"crossref","first-page":"5140","DOI":"10.4049\/jimmunol.171.10.5140","volume":"171","year":"2003","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB16","doi-asserted-by":"crossref","first-page":"2721","DOI":"10.4049\/jimmunol.178.5.2721","volume":"178","year":"2007","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB17","doi-asserted-by":"crossref","first-page":"2748","DOI":"10.1161\/hs1201.099631","volume":"32","year":"2001","journal-title":"Stroke"},{"key":"2026032102293334800_BIB18","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s004300050071","volume":"187","year":"1998","journal-title":"Med Microbiol Immunol (Berl)"},{"key":"2026032102293334800_BIB19","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1191\/1352458503ms944oa","volume":"9","year":"2003","journal-title":"Mult Scler"},{"key":"2026032102293334800_BIB20","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1046\/j.1365-2567.1999.00809.x","volume":"97","year":"1999","journal-title":"Immunology"},{"key":"2026032102293334800_BIB21","doi-asserted-by":"crossref","first-page":"4812","DOI":"10.4049\/jimmunol.164.9.4812","volume":"164","year":"2000","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB22","doi-asserted-by":"crossref","first-page":"2524","DOI":"10.4049\/jimmunol.169.5.2524","volume":"169","year":"2002","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB23","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.4049\/jimmunol.178.2.1048","volume":"178","year":"2007","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB24"},{"key":"2026032102293334800_BIB25","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1126\/science.278.5343.1623","volume":"278","year":"1997","journal-title":"Science"},{"key":"2026032102293334800_BIB26","doi-asserted-by":"crossref","first-page":"4338","DOI":"10.4049\/jimmunol.156.11.4338","volume":"156","year":"1996","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB27","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1002\/eji.200324387","volume":"34","year":"2004","journal-title":"Eur J Immunol"},{"key":"2026032102293334800_BIB28","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1128\/IAI.73.3.1568-1577.2005","volume":"73","year":"2005","journal-title":"Infect Immun"},{"key":"2026032102293334800_BIB29","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S1074-7613(00)80533-6","volume":"8","year":"1998","journal-title":"Immunity"},{"key":"2026032102293334800_BIB30","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1172\/JCI18246","volume":"112","year":"2003","journal-title":"J Clin Invest"},{"key":"2026032102293334800_BIB31","doi-asserted-by":"crossref","first-page":"4345","DOI":"10.4049\/jimmunol.167.8.4345","volume":"167","year":"2001","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB32","doi-asserted-by":"crossref","first-page":"4457","DOI":"10.4049\/jimmunol.170.9.4457","volume":"170","year":"2003","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB33","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1084\/jem.184.2.741","volume":"184","year":"1996","journal-title":"J Exp Med"},{"key":"2026032102293334800_BIB34","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/S0952-7915(97)80100-8","volume":"9","year":"1997","journal-title":"Curr Opin Immunol"},{"key":"2026032102293334800_BIB35","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.1136\/gut.2006.111989","volume":"56","year":"2007","journal-title":"Gut"},{"key":"2026032102293334800_BIB36","doi-asserted-by":"crossref","first-page":"6633","DOI":"10.4049\/jimmunol.164.12.6633","volume":"164","year":"2000","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB37","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/1074-7613(95)90032-2","volume":"2","year":"1995","journal-title":"Immunity"},{"key":"2026032102293334800_BIB38","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/0092-8674(95)90349-6","volume":"80","year":"1995","journal-title":"Cell"},{"key":"2026032102293334800_BIB39","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1046\/j.1365-2249.2002.01700.x","volume":"127","year":"2002","journal-title":"Clin Exp Immunol"},{"key":"2026032102293334800_BIB40","doi-asserted-by":"crossref","first-page":"537","DOI":"10.4049\/jimmunol.168.2.537","volume":"168","year":"2002","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB41","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4049\/jimmunol.163.1.93","volume":"163","year":"1999","journal-title":"J Immunol"},{"key":"2026032102293334800_BIB42","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1038\/ni851","volume":"3","year":"2002","journal-title":"Nat Immunol"},{"key":"2026032102293334800_BIB43","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1084\/jem.20040317","volume":"199","year":"2004","journal-title":"J Exp Med"},{"key":"2026032102293334800_BIB44","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1093\/intimm\/dxh342","volume":"17","year":"2005","journal-title":"Int Immunol"},{"key":"2026032102293334800_BIB45","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1084\/jem.189.7.1121","volume":"189","year":"1999","journal-title":"J Exp Med"},{"key":"2026032102293334800_BIB46","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1002\/jlb.59.4.505","volume":"59","year":"1996","journal-title":"J Leukoc Biol"},{"key":"2026032102293334800_BIB47","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0041-1345(00)01972-2","volume":"33","year":"2001","journal-title":"Transplant Proc"},{"key":"2026032102293334800_BIB48","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1084\/jem.188.5.809","volume":"188","year":"1998","journal-title":"J Exp Med"},{"key":"2026032102293334800_BIB49","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1128\/IAI.70.3.1097-1105.2002","volume":"70","year":"2002","journal-title":"Infect Immun"},{"key":"2026032102293334800_BIB50","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1128\/IAI.67.4.1763-1769.1999","volume":"67","year":"1999","journal-title":"Infect Immun"}],"container-title":["American Journal of Respiratory and Critical Care Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/178\/7\/745\/67444590\/ajrccm_178_7_745.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/178\/7\/745\/67444590\/ajrccm_178_7_745.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T06:29:42Z","timestamp":1774074582000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/article\/178\/7\/745\/8525927"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10,1]]},"references-count":50,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2008,10,1]]},"published-print":{"date-parts":[[2008,10,1]]}},"URL":"https:\/\/doi.org\/10.1164\/rccm.200804-517oc","relation":{},"ISSN":["1073-449X","1535-4970"],"issn-type":[{"value":"1073-449X","type":"print"},{"value":"1535-4970","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2008,10]]},"published":{"date-parts":[[2008,10,1]]}}}