{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:00:46Z","timestamp":1791172846041,"version":"4.1.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Airway remodeling in asthma comprises increased airway smooth muscle (ASM), an alteration linked to airway hyperresponsiveness and disease severity. Experimental studies showed that T cells adhere to ASM through vascular cell adhesion molecule-1 (VCAM-1) and drive ASM growth through direct contact between the T cells and smooth muscle \u03b1-actin (\u03b1-SMA)+ cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To support the hypothesis of a T-cell\/\u03b1-SMA+ cell contact mechanism of ASM remodeling in asthma, using bronchial biopsies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We performed quantitative morphology on T cells, proliferating cell nuclear antigen (PCNA), \u03b1-SMA, and VCAM-1 on biopsies from subjects with moderate and severe asthma and healthy control subjects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Measurements and Main Results<\/jats:title>\n                    <jats:p>We demonstrate ASM cell proliferation and infiltration by T cells in proportion to severity in the subjects with asthma. T cells localized with \u03b1-SMA+PCNA+ cells, suggesting direct intercellular contact and a relationship with \u03b1-SMA+ cell proliferation. Furthermore, the subjects with asthma developed a proliferating compartment of subepithelial \u03b1-SMA+, nonorganized airway contractile elements (NOACE), suggesting a phenotype gradient from undifferentiated cells to smooth muscle\u2013like cells. T-cell juxtaposition events were also observed in this compartment and correlated to its mass. The subjects with asthma showed VCAM-1 expression in postcapillary venules and clusters of VCAM-1 immunoreactivity in ASM and NOACE, consistent with a role of VCAM-1 in T-cell\/\u03b1-SMA+ cell interaction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>T cells may induce \u03b1-SMA+ cell proliferation through direct intercellular contact. NOACE may in part contribute to ASM growth through differentiation and translocation of \u03b1-SMA+ cells. The findings support the role of the T cell in ASM remodeling in asthma.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1164\/rccm.200905-0745oc","type":"journal-article","created":{"date-parts":[[2010,4,15]],"date-time":"2010-04-15T22:40:05Z","timestamp":1271371205000},"page":"317-324","source":"Crossref","is-referenced-by-count":98,"title":["T Cells Localize with Proliferating Smooth Muscle \u03b1-Actin+ Cell Compartments in Asthma"],"prefix":"10.1093","volume":"182","author":[{"given":"David","family":"Ramos-Barb\u00f3n","sequence":"first","affiliation":[{"name":"Respiratory Research Unit, Complexo Hospitalario Universitario and Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Spain"},{"name":"Meakins-Christie Laboratories, McGill University, Montreal, Canada; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebeca","family":"Fraga-Iriso","sequence":"additional","affiliation":[{"name":"Respiratory Research Unit, Complexo Hospitalario Universitario and Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia S.","family":"Brienza","sequence":"additional","affiliation":[{"name":"Respiratory Research Unit, Complexo Hospitalario Universitario and Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Montero-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Respiratory Research Unit, Complexo Hospitalario Universitario and Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9ctor","family":"Verea-Hernando","sequence":"additional","affiliation":[{"name":"Respiratory Research Unit, Complexo Hospitalario Universitario and Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ron","family":"Olivenstein","sequence":"additional","affiliation":[{"name":"Meakins-Christie Laboratories, McGill University, Montreal, Canada; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Lemiere","sequence":"additional","affiliation":[{"name":"H\u00f4pital Sacre Coeur, Montreal, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Ernst","sequence":"additional","affiliation":[{"name":"Meakins-Christie Laboratories, McGill University, Montreal, Canada; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qutayba A.","family":"Hamid","sequence":"additional","affiliation":[{"name":"Meakins-Christie Laboratories, McGill University, Montreal, Canada; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James G.","family":"Martin","sequence":"additional","affiliation":[{"name":"Meakins-Christie Laboratories, 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