{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T15:35:31Z","timestamp":1782142531765,"version":"3.54.5"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Nicotinamide N-methyl transferase (NNMT) is highly expressed in quadriceps muscles of patients with chronic obstructive pulmonary disease (COPD). However, its expression in the diaphragm of these patients has not been assessed. The functional significance of NNMT induction in skeletal muscles of patients with COPD is also unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>(1) To compare NNMT expressions in the diaphragm and quadriceps muscles of patients with COPD. (2) To identify the influence of proinflammatory cytokines on NNMT expression. (3) To assess the influence of NNMT on indices of myogenesis (satellite cell migration and proliferation) and the defense against oxidative stress.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Costal diaphragm muscle biopsies were acquired from 13 patients with moderate and severe COPD and 8 control subjects. Quadriceps muscle biopsies were obtained from 12 patients with COPD and 14 control subjects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Measurements and Main Results<\/jats:title>\n                    <jats:p>NNMT expressions were significantly elevated in the diaphragm and quadriceps muscles of patients with COPD; however, the relative induction of NNMT expression was greater in the quadriceps muscle (10-fold) than it was in the diaphragm (2-fold). NNMT expressions correlated negatively with the severity of COPD and limb muscle wasting. In skeletal myoblasts, NNMT expression was significantly induced by IL-6, transforming growth factor \u03b2, and tumor necrosis factor\u2013\u03b1. Overexpression of NNMT in myoblasts triggered a significant increase in proliferation and migration, but had no influence on cell death. Carbonyl formation, induced by exposing myoblasts to H2O2, was significantly attenuated when NNMT was overexpressed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Up-regulation of NNMT expression in the skeletal muscles of patients with COPD may represent an adaptive response designed to improve myogenesis and defend against oxidative stress.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1164\/rccm.200906-0936oc","type":"journal-article","created":{"date-parts":[[2010,1,28]],"date-time":"2010-01-28T22:45:00Z","timestamp":1264718700000},"page":"797-805","source":"Crossref","is-referenced-by-count":54,"title":["Expression and Functional Significance of Nicotinamide N-methyl Transferase in Skeletal Muscles of Patients with Chronic Obstructive Pulmonary Disease"],"prefix":"10.1093","volume":"181","author":[{"given":"Ho Cheol","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, College of Medicine, Gyeongsang Institute of Health Sciences, Gyeongsang National University, Jinju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahroo","family":"Mofarrahi","sequence":"additional","affiliation":[{"name":"Critical Care and Respiratory Divisions, Royal Victoria Hospital, McGill University Health Centre, Montreal, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theodoros","family":"Vassilakopoulos","sequence":"additional","affiliation":[{"name":"Department of Critical Care and Pulmonary Services, George P. Livanos Laboratory, University of Athens Medical School, Evangelismos Hospital, Athens, Greece; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois","family":"Maltais","sequence":"additional","affiliation":[{"name":"Centre de Recherche, H\u00f4pital Laval, Institut Universitaire de Cardiologie et de Pneumologie de l'Universit\u00e9 Laval, Quebec City, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioanna","family":"Sigala","sequence":"additional","affiliation":[{"name":"Department of Critical Care and Pulmonary Services, George P. Livanos Laboratory, University of Athens Medical School, Evangelismos Hospital, Athens, Greece; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Debigare","sequence":"additional","affiliation":[{"name":"Centre de Recherche, H\u00f4pital Laval, Institut Universitaire de Cardiologie et de Pneumologie de l'Universit\u00e9 Laval, Quebec City, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Bellenis","sequence":"additional","affiliation":[{"name":"Department of Critical Care and Pulmonary Services, George P. Livanos Laboratory, University of Athens Medical School, Evangelismos Hospital, Athens, Greece; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabah N. A.","family":"Hussain","sequence":"additional","affiliation":[{"name":"Critical Care and Respiratory Divisions, Royal Victoria Hospital, McGill University Health Centre, Montreal, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,4,15]]},"reference":[{"key":"2026032504283878700_BIB1","first-page":"75","volume-title":"COPD","year":"2008"},{"key":"2026032504283878700_BIB2","first-page":"601","volume-title":"Arch Biochem Biophys","year":"1988"},{"key":"2026032504283878700_BIB3","first-page":"555","volume-title":"Genomics","year":"1995"},{"key":"2026032504283878700_BIB4","first-page":"412","volume-title":"Atherosclerosis","year":"2008"},{"key":"2026032504283878700_BIB5","first-page":"1799","volume-title":"N Engl J Med","year":"1997"},{"key":"2026032504283878700_BIB6","first-page":"1321","volume-title":"J Cell Biol","year":"2000"},{"key":"2026032504283878700_BIB7","first-page":"3283","volume-title":"Hum Mol Genet","year":"2002"},{"key":"2026032504283878700_BIB8","first-page":"4154","volume-title":"J Immunol","year":"2002"},{"key":"2026032504283878700_BIB9","first-page":"706","volume-title":"Am J Respir Crit Care Med","year":"2003"},{"key":"2026032504283878700_BIB10","first-page":"551","volume-title":"J Cancer Res Clin Oncol","year":"2008"},{"key":"2026032504283878700_BIB11","first-page":"675","volume-title":"Biochem Biophys Res Commun","year":"2002"},{"key":"2026032504283878700_BIB12","first-page":"674","volume-title":"Exp Clin Endocrinol Diabetes","year":"2007"},{"key":"2026032504283878700_BIB13","first-page":"17147","volume-title":"J Biol Chem","year":"2008"},{"key":"2026032504283878700_BIB14","first-page":"637","volume-title":"Int J Chron Obstruct Pulmon Dis","year":"2008"},{"key":"2026032504283878700_BIB15","first-page":"82","volume-title":"Biochim Biophys Acta","year":"1971"},{"key":"2026032504283878700_BIB16","first-page":"127","volume-title":"Arch Immunol Ther Exp (Warsz)","year":"2008"},{"key":"2026032504283878700_BIB17","first-page":"6679","volume-title":"Oncogene","year":"2008"},{"key":"2026032504283878700_BIB18","first-page":"518","volume-title":"Biochim Biophys Acta","year":"1982"},{"key":"2026032504283878700_BIB19","first-page":"A274","volume-title":"Am J Respir Crit Care Med","year":"2008"},{"key":"2026032504283878700_BIB20","first-page":"1","volume-title":"Eur Respir J","year":"2003"},{"key":"2026032504283878700_BIB21","first-page":"609","volume-title":"Scand J Clin Lab Invest","year":"1975"},{"key":"2026032504283878700_BIB22","first-page":"L955","volume-title":"Am J Physiol Lung Cell Mol Physiol","year":"2008"},{"key":"2026032504283878700_BIB23","first-page":"559","volume-title":"Antioxid Redox Signal","year":"2008"},{"key":"2026032504283878700_BIB24","first-page":"4145","volume-title":"Blood","year":"2008"},{"key":"2026032504283878700_BIB25","first-page":"9","volume-title":"Am J Respir Cell Mol Biol","year":"2005"},{"key":"2026032504283878700_BIB26","first-page":"186","volume-title":"Exp Cell Res","year":"1999"},{"key":"2026032504283878700_BIB27","first-page":"1205","volume-title":"J Appl Physiol","year":"2002"},{"key":"2026032504283878700_BIB28","first-page":"371","volume-title":"Eur Respir J","year":"1999"},{"key":"2026032504283878700_BIB29","first-page":"390","volume-title":"Eur Respir J","year":"2005"},{"key":"2026032504283878700_BIB30","first-page":"819","volume-title":"Thorax","year":"1996"},{"key":"2026032504283878700_BIB31","first-page":"574","volume-title":"Thorax","year":"2004"},{"key":"2026032504283878700_BIB32","first-page":"1218","volume-title":"Am J Respir Crit Care Med","year":"2002"},{"key":"2026032504283878700_BIB33","first-page":"1012","volume-title":"Br J Cancer","year":"2002"},{"key":"2026032504283878700_BIB34","first-page":"1630","volume-title":"FASEB J","year":"2002"},{"key":"2026032504283878700_BIB35","first-page":"527","volume-title":"Mol Endocrinol","year":"2005"},{"key":"2026032504283878700_BIB36","first-page":"414","volume-title":"Mol Cell Biol","year":"2001"},{"key":"2026032504283878700_BIB37","first-page":"6550","volume-title":"Clin Cancer Res","year":"2005"},{"key":"2026032504283878700_BIB38","first-page":"377","volume-title":"J Pathol","year":"2005"},{"key":"2026032504283878700_BIB39","first-page":"415","volume-title":"Mol Med","year":"2007"},{"key":"2026032504283878700_BIB40","first-page":"534","volume-title":"J Appl Physiol","year":"2001"},{"key":"2026032504283878700_BIB41","first-page":"239","volume-title":"Arch Bronconeumol","year":"2008"},{"key":"2026032504283878700_BIB42","first-page":"98","volume-title":"Am J Respir Crit Care Med","year":"2000"},{"key":"2026032504283878700_BIB43","first-page":"771","volume-title":"Am J Respir Cell Mol Biol","year":"2003"},{"key":"2026032504283878700_BIB44","first-page":"383","volume-title":"Muscle Nerve","year":"2002"},{"key":"2026032504283878700_BIB45","first-page":"1664","volume-title":"Am J Respir Crit Care Med","year":"2003"},{"key":"2026032504283878700_BIB46","first-page":"1116","volume-title":"Am J Respir Crit Care Med","year":"2005"}],"container-title":["American Journal of Respiratory and Critical Care Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/181\/8\/797\/67506487\/ajrccm_181_8_797.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/181\/8\/797\/67506487\/ajrccm_181_8_797.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T08:34:01Z","timestamp":1774427641000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/article\/181\/8\/797\/8515520"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":46,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2010,4,15]]},"published-print":{"date-parts":[[2010,4,15]]}},"URL":"https:\/\/doi.org\/10.1164\/rccm.200906-0936oc","relation":{},"ISSN":["1073-449X","1535-4970"],"issn-type":[{"value":"1073-449X","type":"print"},{"value":"1535-4970","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2010,4]]},"published":{"date-parts":[[2010,4]]}}}