{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:18:26Z","timestamp":1787509106007,"version":"build-2736575974"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>The airway luminal memory CD8 T cells induced by respiratory mucosal immunization in a murine model have been found to be critical to antituberculosis immunity. However, the mechanisms of their maintenance on airway mucosal surface still remain poorly understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Using a model of adenovirus-based intranasal immunization we investigated the immune property and the mechanisms of maintenance of airway luminal CD8 T cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immune properties of airway luminal Mycobacterium tuberculosis antigen\u2013specific CD8 T cells were examined. Proliferation of airway luminal CD8 T cells was determined by in vivo T cell\u2013labeling techniques. The role of peripheral T cell recruitment in maintaining airway luminal CD8 T cells was investigated by blocking lymphocyte trafficking from lymphoid and peripheral tissues. The requirement of M. tuberculosis antigens for in situ T cell proliferation was evaluated using a T cell transfer approach. An airway M. tuberculosis challenge model was used to study the relationship between CD8 T cell\u2013mediated protection and peripheral T cell recruitment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Measurements and Main Results<\/jats:title>\n                    <jats:p>Intranasal immunization leads to elicitation of persisting M. tuberculosis antigen\u2013specific CD8 T cells in the airway lumen, which display an activated effector memory phenotype different from those in peripheral tissues. Airway luminal T cells continuously proliferate in an antigen-dependent manner, and can be maintained even in the absence of peripheral T cell recruitment. The lungs equipped with such CD8 T cells are protected from airway M. tuberculosis challenge independent of both peripheral T cell supply and CD4 T cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Vaccine-inducible airway luminal antituberculosis memory CD8 T cells are self-renewable in an antigen-dependent manner, and can be maintained independent of peripheral T cell supply.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1164\/rccm.200910-1583oc","type":"journal-article","created":{"date-parts":[[2009,12,18]],"date-time":"2009-12-18T02:56:48Z","timestamp":1261105008000},"page":"862-872","source":"Crossref","is-referenced-by-count":56,"title":["Murine Airway Luminal Antituberculosis Memory CD8 T Cells by Mucosal Immunization Are Maintained Via Antigen-Driven\n                    <i>In Situ<\/i>\n                    Proliferation, Independent of Peripheral T Cell Recruitment"],"prefix":"10.1093","volume":"181","author":[{"given":"Mangalakumari","family":"Jeyanathan","sequence":"first","affiliation":[{"name":"Centre for Gene Therapeutics, M. G. DeGroote Institute for Infectious Disease Research, and Department of Pathology & Molecular Medicine, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyu","family":"Mu","sequence":"additional","affiliation":[{"name":"Centre for Gene Therapeutics, M. G. DeGroote Institute for Infectious Disease Research, and Department of Pathology & Molecular Medicine, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"McCormick","sequence":"additional","affiliation":[{"name":"Centre for Gene Therapeutics, M. G. DeGroote Institute for Infectious Disease Research, and Department of Pathology & Molecular Medicine, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Damjanovic","sequence":"additional","affiliation":[{"name":"Centre for Gene Therapeutics, M. G. DeGroote Institute for Infectious Disease Research, and Department of Pathology & Molecular Medicine, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cherrie-Lee","family":"Small","sequence":"additional","affiliation":[{"name":"Centre for Gene Therapeutics, M. G. DeGroote Institute for Infectious Disease Research, and Department of Pathology & Molecular Medicine, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher R.","family":"Shaler","sequence":"additional","affiliation":[{"name":"Centre for Gene Therapeutics, M. G. DeGroote Institute for Infectious Disease Research, and Department of Pathology & Molecular Medicine, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kapilan","family":"Kugathasan","sequence":"additional","affiliation":[{"name":"Centre for Gene Therapeutics, M. G. DeGroote Institute for Infectious Disease Research, and Department of Pathology & Molecular Medicine, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Xing","sequence":"additional","affiliation":[{"name":"Centre for Gene Therapeutics, M. G. DeGroote Institute for Infectious Disease Research, and Department of Pathology & Molecular Medicine, McMaster University, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,4,15]]},"reference":[{"key":"2026032504284976600_BIB1","first-page":"S45","volume-title":"Nat Med","year":"2005"},{"key":"2026032504284976600_BIB2","first-page":"257","volume-title":"Tuberculosis (Edinb)","year":"2007"},{"key":"2026032504284976600_BIB3","first-page":"148","volume-title":"Natl Rev","year":"2006"},{"key":"2026032504284976600_BIB4","first-page":"211","volume-title":"Tuberculosis (Edinb)","year":"2006"},{"key":"2026032504284976600_BIB5","first-page":"385","volume-title":"Curr Drug Deliv","year":"2004"},{"key":"2026032504284976600_BIB6","first-page":"105","volume-title":"J Biotechnol","year":"2000"},{"key":"2026032504284976600_BIB7","first-page":"932","volume-title":"Microbes Infect","year":"2005"},{"key":"2026032504284976600_BIB8","first-page":"153","volume-title":"Natl Rev","year":"2009"},{"key":"2026032504284976600_BIB9","first-page":"4976","volume-title":"J Immunol","year":"2002"},{"key":"2026032504284976600_BIB10","first-page":"4721","volume-title":"J Immunol","year":"2007"},{"key":"2026032504284976600_BIB11","first-page":"1165","volume-title":"Expert Rev Vaccines","year":"2008"},{"key":"2026032504284976600_BIB12","first-page":"393","volume-title":"Annu Rev Immunol","year":"2009"},{"key":"2026032504284976600_BIB13","first-page":"317","volume-title":"Crit Rev Immunol","year":"2006"},{"key":"2026032504284976600_BIB14","first-page":"6361","volume-title":"J Immunol","year":"2006"},{"key":"2026032504284976600_BIB15","first-page":"4590","volume-title":"J Immunol","year":"2004"},{"key":"2026032504284976600_BIB16","first-page":"1685","volume-title":"Infect Immun","year":"2004"},{"key":"2026032504284976600_BIB17","first-page":"271","volume-title":"J Exp Med","year":"2001"},{"key":"2026032504284976600_BIB18","first-page":"4320","volume-title":"Infect Immun","year":"2001"},{"key":"2026032504284976600_BIB19","first-page":"6357","volume-title":"J Immunol","year":"2004"},{"key":"2026032504284976600_BIB20","first-page":"4634","volume-title":"Infect Immun","year":"2006"},{"key":"2026032504284976600_BIB21","first-page":"4955","volume-title":"J Immunol","year":"2008"},{"key":"2026032504284976600_BIB22","first-page":"1602","volume-title":"J Immunol","year":"2003"},{"key":"2026032504284976600_BIB23","first-page":"5618","volume-title":"J Immunol","year":"2008"},{"key":"2026032504284976600_BIB24","first-page":"2387","volume-title":"J Immunol","year":"2007"},{"key":"2026032504284976600_BIB25","first-page":"7986","volume-title":"J Immunol","year":"2005"},{"key":"2026032504284976600_BIB26","first-page":"643","volume-title":"Immunity","year":"2006"},{"key":"2026032504284976600_BIB27","first-page":"5837","volume-title":"J Immunol","year":"2008"},{"key":"2026032504284976600_BIB28","first-page":"5","volume-title":"Immunity","year":"2004"},{"key":"2026032504284976600_BIB29","first-page":"426","volume-title":"Nat Immunol","year":"2000"},{"key":"2026032504284976600_BIB30","first-page":"848","volume-title":"Immunity","year":"2008"},{"key":"2026032504284976600_BIB31","first-page":"1209","volume-title":"J Immunol","year":"2004"},{"key":"2026032504284976600_BIB32","first-page":"4177","volume-title":"J Immunol","year":"2009"},{"key":"2026032504284976600_BIB33","first-page":"265","volume-title":"Immunity","year":"2003"},{"key":"2026032504284976600_BIB34","first-page":"439","volume-title":"Immunity","year":"2006"},{"key":"2026032504284976600_BIB35","first-page":"2039","volume-title":"J Virol","year":"2007"},{"key":"2026032504284976600_BIB36","first-page":"3662","volume-title":"J Immunol","year":"2003"},{"key":"2026032504284976600_BIB37","first-page":"244","volume-title":"Curr Opin Pharmacol","year":"2006"},{"key":"2026032504284976600_BIB38","first-page":"496","volume-title":"Biochim Biophys Acta","year":"2008"},{"key":"2026032504284976600_BIB39","first-page":"13407","volume-title":"J Virol","year":"2003"},{"key":"2026032504284976600_BIB40","first-page":"163","volume-title":"Tuberculosis (Edinb)","year":"2006"},{"key":"2026032504284976600_BIB41","first-page":"962","volume-title":"Microbes Infect","year":"2005"},{"key":"2026032504284976600_BIB42","first-page":"539","volume-title":"Expert Rev Vaccines","year":"2007"},{"key":"2026032504284976600_BIB43","first-page":"776","volume-title":"Microbes Infect","year":"2005"},{"key":"2026032504284976600_BIB44","first-page":"e5528","volume-title":"PLoS One","year":"2009"},{"key":"2026032504284976600_BIB45","first-page":"317","volume-title":"J Exp Med","year":"2002"},{"key":"2026032504284976600_BIB46","first-page":"616","volume-title":"Mol Ther","year":"2004"},{"key":"2026032504284976600_BIB47","first-page":"1916","volume-title":"Blood","year":"2007"},{"key":"2026032504284976600_BIB48","first-page":"271","volume-title":"Immunity","year":"2002"},{"key":"2026032504284976600_BIB49","first-page":"48","volume-title":"BMC Immunol","year":"2008"},{"key":"2026032504284976600_BIB50","first-page":"4051","volume-title":"J Immunol","year":"2006"}],"container-title":["American Journal of Respiratory and Critical Care Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/181\/8\/862\/67506428\/ajrccm_181_8_862.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/181\/8\/862\/67506428\/ajrccm_181_8_862.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T08:34:33Z","timestamp":1774427673000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/article\/181\/8\/862\/8515511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":50,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2010,4,15]]},"published-print":{"date-parts":[[2010,4,15]]}},"URL":"https:\/\/doi.org\/10.1164\/rccm.200910-1583oc","relation":{},"ISSN":["1073-449X","1535-4970"],"issn-type":[{"value":"1073-449X","type":"print"},{"value":"1535-4970","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2010,4]]},"published":{"date-parts":[[2010,4]]}}}