{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T11:01:20Z","timestamp":1789556480766,"version":"build-2803163510"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Cystic fibrosis (CF) airways disease produces a mucoobstructive lung phenotype characterized by airways mucus plugging, epithelial mucous cell metaplasia\/hyperplasia, chronic infection, and inflammation. Simultaneous biochemical and functional in vivo studies of mucin synthesis and secretion from CF airways are not available. In vitro translational models may quantitate differential CF versus normal mucin and fluid secretory responses to infectious\/inflammatory stimuli.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>We tested the hypothesis that CF airways exhibit defective epithelial fluid, but not mucin, secretory responses to bacterial\/inflammatory host products.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Well-differentiated primary human bronchial epithelial cultures were exposed to supernatant from mucopurulent material (SMM) from human CF airways as a test of bacterial\/inflammatory host product stimulus. Human bronchial epithelia (HBE) with normal CF transmembrane conductance regulator function were compared with \u0394F508\/\u0394F508 CF HBE.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Measurements and Main Results<\/jats:title>\n                    <jats:p>Acute (up to 60 min) SMM exposure promoted mucin secretion, but mucins were degraded by the proteolytic enzymes present in SMM. Chronic SMM exposure induced upregulation of mucin synthesis and storage and generated absolute increases in basal and stimulated mucin release in normal and CF cultures. These responses were similar in normal and CF cultures. In contrast, SMM produced a coordinated CF transmembrane conductance regulator\u2013mediated Cl\u2212 secretory response in normal HBE, but not in CF HBE. The absence of the fluid secretory response in CF produced quantitatively more dehydrated mucus.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study reveals the interplay between regulation of mucin and fluid secretion rates in inflamed versus noninflamed conditions and why a hyperconcentrated mucus is produced in CF airways.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1164\/rccm.201706-1139oc","type":"journal-article","created":{"date-parts":[[2017,11,3]],"date-time":"2017-11-03T15:27:21Z","timestamp":1509722841000},"page":"481-491","source":"Crossref","is-referenced-by-count":45,"title":["Mucin Production and Hydration Responses to Mucopurulent Materials in Normal versus Cystic Fibrosis Airway Epithelia"],"prefix":"10.1093","volume":"197","author":[{"given":"Lubna H.","family":"Abdullah","sequence":"first","affiliation":[{"name":"Marsico Lung Institute and Cystic Fibrosis Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond","family":"Coakley","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute and Cystic Fibrosis Research Center"},{"name":"Department of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan J.","family":"Webster","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute and Cystic Fibrosis Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunxiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute and Cystic Fibrosis Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Tarran","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute and Cystic Fibrosis Research Center"},{"name":"Department of Cell Biology and Physiology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Radicioni","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute and Cystic Fibrosis Research Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet","family":"Kesimer","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute and Cystic Fibrosis Research Center"},{"name":"Department of Pathology and Laboratory Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard C.","family":"Boucher","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute and Cystic Fibrosis Research Center"},{"name":"Department of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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