{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T22:35:42Z","timestamp":1785882942632,"version":"3.56.0"},"reference-count":13,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":600,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Gift from Winn-Barlow"},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P01HL158505"],"award-info":[{"award-number":["P01HL158505"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL161620"],"award-info":[{"award-number":["R01HL161620"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35HL150876"],"award-info":[{"award-number":["R35HL150876"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32HL007633"],"award-info":[{"award-number":["T32HL007633"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Gail and Adam Slifka"},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["KIM19P0"],"award-info":[{"award-number":["KIM19P0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["KIM21G0"],"award-info":[{"award-number":["KIM21G0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001021","name":"Damon Runyon Cancer Research Foundation","doi-asserted-by":"publisher","award":["DRG:2368-19"],"award-info":[{"award-number":["DRG:2368-19"]}],"id":[{"id":"10.13039\/100001021","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Gilda and Alfred Slifka","award":["K23HL158068"],"award-info":[{"award-number":["K23HL158068"]}]},{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Fund","doi-asserted-by":"publisher","award":["1019903"],"award-info":[{"award-number":["1019903"]}],"id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014780","name":"Longfonds","doi-asserted-by":"publisher","award":["Accelerate Project BREATH"],"award-info":[{"award-number":["Accelerate Project BREATH"]}],"id":[{"id":"10.13039\/501100014780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008035","name":"Harvard Stem Cell Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008035","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Cystic Fibrosis\/Multiple Sclerosis Fund Foundation"},{"name":"Gift from Winn-Barlow"},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P01HL158505"],"award-info":[{"award-number":["P01HL158505"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL161620"],"award-info":[{"award-number":["R01HL161620"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35HL150876"],"award-info":[{"award-number":["R35HL150876"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32HL007633"],"award-info":[{"award-number":["T32HL007633"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Gail and Adam Slifka"},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["KIM19P0"],"award-info":[{"award-number":["KIM19P0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000897","name":"Cystic Fibrosis Foundation","doi-asserted-by":"publisher","award":["KIM21G0"],"award-info":[{"award-number":["KIM21G0"]}],"id":[{"id":"10.13039\/100000897","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001021","name":"Damon Runyon Cancer Research Foundation","doi-asserted-by":"publisher","award":["DRG:2368-19"],"award-info":[{"award-number":["DRG:2368-19"]}],"id":[{"id":"10.13039\/100001021","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"Gilda and Alfred Slifka","doi-asserted-by":"publisher","award":["K23HL158068"],"award-info":[{"award-number":["K23HL158068"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Fund","doi-asserted-by":"publisher","award":["1019903"],"award-info":[{"award-number":["1019903"]}],"id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014780","name":"Longfonds","doi-asserted-by":"publisher","award":["Accelerate Project BREATH"],"award-info":[{"award-number":["Accelerate Project BREATH"]}],"id":[{"id":"10.13039\/501100014780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008035","name":"Harvard Stem Cell Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008035","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Cystic Fibrosis\/Multiple Sclerosis Fund Foundation"}],"content-domain":{"domain":["www.atsjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,6,15]]},"DOI":"10.1164\/rccm.202310-1831le","type":"journal-article","created":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T10:58:55Z","timestamp":1713869935000},"page":"1501-1504","update-policy":"https:\/\/doi.org\/10.1164\/crossmarkpolicies","source":"Crossref","is-referenced-by-count":19,"title":["Human Airway and Alveolar Organoids from BAL Fluid"],"prefix":"10.1093","volume":"209","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3936-9377","authenticated-orcid":false,"given":"Monica Yun","family":"Liu","sequence":"first","affiliation":[{"name":"Stem Cell Program, Division of Hematology\/Oncology, and"},{"name":"Division of Pulmonary and Critical Care Medicine and"},{"name":"Department of Medicine, University of Wisconsin\u2013Madison School of Medicine and Public Health, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belinda","family":"Chen","sequence":"additional","affiliation":[{"name":"Stem Cell Program, Division of Hematology\/Oncology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7023-434X","authenticated-orcid":false,"given":"Mehdi","family":"Borji","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts"},{"name":"Broad Institute of MIT and Harvard, Cambridge, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9774-445X","authenticated-orcid":false,"given":"Carolina","family":"Garcia de Alba Rivas","sequence":"additional","affiliation":[{"name":"Stem Cell Program, Division of Hematology\/Oncology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8705-2409","authenticated-orcid":false,"given":"Antonella F. M.","family":"Dost","sequence":"additional","affiliation":[{"name":"Stem Cell Program, Division of Hematology\/Oncology, and"},{"name":"Hubrecht Institute for Developmental Biology and Stem Cell Research, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, Utrecht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1004-5889","authenticated-orcid":false,"given":"Aaron L.","family":"Moye","sequence":"additional","affiliation":[{"name":"Stem Cell Program, Division of Hematology\/Oncology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nusrath","family":"Movval Abdulla","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margherita","family":"Paschini","sequence":"additional","affiliation":[{"name":"Stem Cell Program, Division of Hematology\/Oncology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9861-2409","authenticated-orcid":false,"given":"Stuart D.","family":"Rollins","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Medicine, Boston Children\u2019s Hospital, Boston, Massachusetts"},{"name":"Department of Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0277-8329","authenticated-orcid":false,"given":"Ruobing","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Medicine, Boston Children\u2019s Hospital, Boston, Massachusetts"},{"name":"Department of Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9189-7567","authenticated-orcid":false,"given":"Lynn M.","family":"Schnapp","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Wisconsin\u2013Madison School of Medicine and Public Health, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3835-1290","authenticated-orcid":false,"given":"Hassan A.","family":"Khalil","sequence":"additional","affiliation":[{"name":"Department of Surgery, Brigham and Women\u2019s Hospital, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3348-5054","authenticated-orcid":false,"given":"Catherine J.","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts"},{"name":"Broad Institute of MIT and Harvard, Cambridge, Massachusetts"},{"name":"Department of Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6340-4844","authenticated-orcid":false,"given":"Nirmal S.","family":"Sharma","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Medicine and"},{"name":"Division of Pulmonary and Critical Care, Veterans Affairs Medical Center, West Roxbury, Boston, Massachusetts; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2366-9538","authenticated-orcid":false,"given":"Carla F.","family":"Kim","sequence":"additional","affiliation":[{"name":"Stem Cell Program, Division of Hematology\/Oncology, and"},{"name":"Division of Pulmonary Medicine, Boston Children\u2019s Hospital, Boston, Massachusetts"},{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts"},{"name":"Harvard Stem Cell Institute, Cambridge, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,6,15]]},"reference":[{"key":"2026012204231718900_bib1","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/j.molmed.2021.09.008","article-title":"Organoid models: assessing lung cell fate decisions and disease responses","volume":"27","author":"Liberti","year":"2021","journal-title":"Trends Mol Med"},{"key":"2026012204231718900_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15252\/embj.2018100300","article-title":"Long-term expanding human airway organoids for disease modeling","volume":"38","author":"Sachs","year":"2019","journal-title":"EMBO J"},{"key":"2026012204231718900_bib3","first-page":"A4690","article-title":"Establishing airway lung organoids from human bronchoalveolar lavage fluid [abstract]","volume":"207","author":"Liu","year":"2023","journal-title":"Am J Respir Crit Care Med"},{"key":"2026012204231718900_bib4","doi-asserted-by":"crossref","first-page":"42214","DOI":"10.1038\/srep42214","article-title":"Novel flow cytometry approach to identify bronchial epithelial cells from healthy human airways","volume":"7","author":"Maestre-Batlle","year":"2017","journal-title":"Sci Rep"},{"key":"2026012204231718900_bib5","doi-asserted-by":"crossref","first-page":"101447","DOI":"10.1016\/j.xpro.2022.101447","article-title":"Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures","volume":"3","author":"Konishi","year":"2022","journal-title":"STAR Protoc"},{"key":"2026012204231718900_bib6","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1016\/j.stem.2020.10.005","article-title":"Human lung stem cell-based alveolospheres provide insights into SARS-CoV-2-mediated interferon responses and pneumocyte dysfunction","volume":"27","author":"Katsura","year":"2020","journal-title":"Cell Stem Cell"},{"key":"2026012204231718900_bib7","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1164\/rccm.202205-1010LE","article-title":"De novo generation of pulmonary ionocytes from normal and cystic fibrosis human induced pluripotent stem cells","volume":"207","author":"Wang","year":"2023","journal-title":"Am J Respir Crit Care Med"},{"key":"2026012204231718900_bib8","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/s13059-017-1382-0","article-title":"SCANPY: large-scale single-cell gene expression data analysis","volume":"19","author":"Wolf","year":"2018","journal-title":"Genome Biol"},{"key":"2026012204231718900_bib9","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1093\/bioinformatics\/btz625","article-title":"BBKNN: fast batch alignment of single cell transcriptomes","volume":"36","author":"Pola\u0144ski","year":"2020","journal-title":"Bioinformatics"},{"key":"2026012204231718900_bib10","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1038\/s41591-023-02327-2","article-title":"An integrated cell atlas of the lung in health and disease","volume":"29","author":"Sikkema","year":"2023","journal-title":"Nat Med"},{"key":"2026012204231718900_bib11","doi-asserted-by":"crossref","first-page":"1636","DOI":"10.1164\/rccm.201911-2199OC","article-title":"A single-cell atlas of the human healthy airways","volume":"202","author":"Deprez","year":"2020","journal-title":"Am J Respir Crit Care Med"},{"key":"2026012204231718900_bib12","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1038\/s41586-018-0393-7","article-title":"A revised airway epithelial hierarchy includes CFTR-expressing ionocytes","volume":"560","author":"Montoro","year":"2018","journal-title":"Nature"},{"key":"2026012204231718900_bib13","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1038\/s41586-018-0394-6","article-title":"A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte","volume":"560","author":"Plasschaert","year":"2018","journal-title":"Nature"}],"container-title":["American Journal of Respiratory and Critical Care Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/209\/12\/1501\/66425373\/ajrccm_209_12_1501.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrccm\/article-pdf\/209\/12\/1501\/66425373\/ajrccm_209_12_1501.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T09:23:39Z","timestamp":1769073819000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrccm\/article\/209\/12\/1501\/8426630"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":13,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,6,15]]},"published-print":{"date-parts":[[2024,6,15]]}},"URL":"https:\/\/doi.org\/10.1164\/rccm.202310-1831le","relation":{},"ISSN":["1073-449X","1535-4970"],"issn-type":[{"value":"1073-449X","type":"print"},{"value":"1535-4970","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,6]]},"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"2024-06-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}