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We tested the hypothesis that the potent CD4+ T cell antigen Mtb39 is also a CD8+ T cell antigen. A recombinant adenovirus\u2013expressing Mtb39 (adenoMtb39) was used to infect monocyte-derived dendritic cells. Using interferon-\u03b3 enzyme-linked immunospot, Mtb39-specific CD8+ T lymphocytes were detected in three healthy individuals with latent tuberculosis infection who also had strong anti\u2013Mtb39-specific CD4+ T cell responses. An Mtb39-specific CD8+ T cell line was generated using Mtb39-expressing dendritic cells. Mtb39-specific T cell clones were obtained by limiting dilution cloning. All seven T cell clones obtained were HLA-B44 restricted. Using a panel of synthetic overlapping peptides representative of Mtb39, the peptide epitope was identified for two clones. Furthermore, all T cell clones recognized Mtb-infected dendritic cells and were cytolytic. We conclude that infection of dendritic cells with adenoviral vectors expressing Mtb proteins allows for measurement of antigen-specific CD8+ T cell responses from peripheral blood mononuclear cells. The technique will be useful in defining CD8+ T cell antigens and in measuring immunogenicity of tuberculosis vaccines.<\/jats:p>","DOI":"10.1164\/rccm.2110094","type":"journal-article","created":{"date-parts":[[2002,9,13]],"date-time":"2002-09-13T17:22:24Z","timestamp":1031937744000},"page":"843-848","source":"Crossref","is-referenced-by-count":25,"title":["Human Dendritic Cells Presenting Adenovirally Expressed Antigen Elicit\n                    <i>Mycobacterium tuberculosis<\/i>\n                    \u2013Specific CD8\n                    <b>+<\/b>\n                    T Cells"],"prefix":"10.1093","volume":"166","author":[{"given":"Deborah A.","family":"Lewinsohn","sequence":"first","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Department of Molecular Microbiology and Immunology; and Division of Pulmonary and Critical Care Medicine, Oregon Health and Science University\/Portland VA Medical Center, Portland, Oregon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca A.","family":"Lines","sequence":"additional","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Department of Molecular Microbiology and Immunology; and Division of Pulmonary and Critical Care Medicine, Oregon Health and Science University\/Portland VA Medical Center, Portland, Oregon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Lewinsohn","sequence":"additional","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Department of Molecular Microbiology and Immunology; and Division of Pulmonary and Critical Care Medicine, Oregon Health and Science University\/Portland VA Medical Center, Portland, 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