{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:35:03Z","timestamp":1740170103422,"version":"3.37.3"},"reference-count":1,"publisher":"Magnolia Press","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zootaxa"],"abstract":"<jats:p>Some nematologists recently placed the genus Pratylenchoides, (\u201cLesion Nematode-like\u201d) in the family Merliniidae. To investigate Pratylenchoides species diversity and their relationships with other Merliniidae genera, specimens were collected from various habitats in the northern and northwestern provinces of Iran. The morphological and molecular study yielded three species of the genus Pratylenchoides, including P. persicus n. sp. This new species is characterized by having lip region rounded or slightly flattened anteriorly with four or five fine but distinct annuli, pharyngeal glands off-set or slightly overlapping the intestine dorsally, all three gland nuclei located anterior to the pharyngo-intestinal valve and tail cylindrical with truncate to low rounded terminus. Morphologically, P. persicus n. sp. can be distinguished from the most closely related species, P. heathi by having shorter body and stylet length in females and males, as well as a shorter tail with different terminus in females. Pratylenchoides laticauda and P. cf. nevadensis are reported from Iran for the first time. Phylogenetic analysis based on the D2\/D3 region of the large subunit of ribosomal DNA revealed Pratylenchoides as a monophyletic genus, and it supports the delineation of the new species, P. persicus n. sp.\u00a0<\/jats:p>","DOI":"10.11646\/zootaxa.4205.5.4","type":"journal-article","created":{"date-parts":[[2016,12,9]],"date-time":"2016-12-09T00:59:19Z","timestamp":1481245159000},"source":"Crossref","is-referenced-by-count":3,"title":["Morphological and molecular characterization of Pratylenchoides persicus n. sp. (Nematoda: Merliniidae) and additional data on two other species of the genus from Iran"],"prefix":"10.11646","volume":"4205","author":[{"given":"KOUROSH","family":"AZIZI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ALI","family":"ESKANDARI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AKBAR","family":"KAREGAR","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"REZA","family":"GHADERI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SVEN VAN DEN","family":"ELSEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARTIJN","family":"HOLTERMAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JOHANNES","family":"HELDER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4913","published-online":{"date-parts":[[2016,12,9]]},"reference":[{"key":"39564","unstructured":"<p class=\"HeadingRunIn\">Baldwin, G., Luc, M. &amp; Bell, A.H. (1983) Contribution to the study of the genus <em>Pratylenchoides<\/em> Winslow (Nematoda: Tylenchida). <em>Revue de N\u00e9matologie<\/em>, 6, 111\u2013125.<\/p><p class=\"HeadingRunIn\">Bert, W., Leliaert, F., Vierstraete, A.R., Vanfleteren, J.R. &amp; Borgonie, G. (2008) Molecular phylogeny of the Tylenchina and evolution of the female gonoduct (Nematoda: Rhabditida). <em>Molecular Phylogenetics and Evolution<\/em>, 48, 728\u2013744.<br \/> <a href=\"http:\/\/dx.doi.org\/10.1016\/j.ympev.2008.04.011\">http:\/\/dx.doi.org\/10.1016\/j.ympev.2008.04.011<\/a><\/p><p class=\"HeadingRunIn\">Braun, A.L. &amp; Loof, P.A.A. (1966) <em>Pratylenchoides laticauda<\/em> n. sp., a new endoparasitic phytonematode. <em>Netherland Journal of Plant Pathology<\/em>, 72, 241\u2013245.<br \/> http:\/\/dx.doi.org\/10.1007\/BF02650210<\/p><p class=\"HeadingRunIn\">Brzeski, M.W. (1998) <em>Nematodes of Tylenchina in Poland and Temperate Europe<\/em>. Warszawa, Poland, Muzeum I Instytut Zoologii PAN, 397 pp.<\/p><p class=\"HeadingRunIn\">De Grisse, A. (1969) Redescription ou modification de quelques techniques utilisees dans \u00e9tude des nematodes phytoparasitaires. <em>Meded Rijksfaculteit der Landbouwetenschappen Gent<\/em>, 34, 351\u2013369.<\/p><p class=\"HeadingRunIn\">Decraemer, W. &amp; Hunt, D.J. (2006) Structure and Classification. <em>In<\/em>: Perry, R.N. &amp; Moens, M. (Eds.), <em>Plant Nematology<\/em>. King's Lynn, UK, CABI Publishing, pp. 3\u201332.<br \/> http:\/\/dx.doi.org\/10.1079\/9781845930561.0003<\/p><p class=\"HeadingRunIn\">Geraert, E. (2013) <em>The Pratylenchidae of the World, Identification of the Family Pratylenchidae (Nematoda: Tylenchida)<\/em>. Gent, Belgium, Academia Press, 430 pp.<\/p><p class=\"HeadingRunIn\">Ghaderi, R. &amp; Karegar, A. (2014) Contribution to a revision of the genus <em>Pratylenchoides<\/em> Winslow, 1958 (Nematoda: Merliniidae), with redescription of <em>P. erzurumensis<\/em> Y\u00fcksel, 1977 from Iran. <em>Zootaxa<\/em>, 3900 (3), 339\u2013369.<br \/> http:\/\/dx.doi.org\/10.11646\/zootaxa.3900.3.2<\/p><p class=\"HeadingRunIn\">Ghaderi, R., Karegar, A., Niknam, G. &amp; Subbotin, S. (2014) Phylogenetic relationships of Telotylenchidae Siddiqi, 1960 and Merliniidae Siddiqi, 1971 (Nematoda: Tylenchida) from Iran, as inferred from the analysis of the D2D3 expansion fragments of 28S rRNA gene sequences. <em>Nematology<\/em>, 16, 863\u2013877.<br \/> http:\/\/dx.doi.org\/10.1163\/15685411-00002815<\/p><p class=\"HeadingRunIn\">Ghahramani Nejad Mianeji, E., Niknam, Gh. &amp; Tanha Maafi, Z. (2011) <em>Pratylenchoides magnicauda<\/em>, <em>Trophurus minnesotensis<\/em> and <em>Xiphinema basilgoodeyi<\/em> as new records for nematode fauna of Iran from farmlands and orchards in Ardebil plain. <em>Applied Entomology and Phytopathology<\/em>, 79, 237\u2013250. [In Persian with English summary]<\/p><p class=\"HeadingRunIn\">Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT. <em>Nucleic Acids Symposium Series,<\/em> 41, 95\u201398.<\/p><p class=\"HeadingRunIn\">Hassanzadeh Khalifehkandi, Z., Karegar, A. &amp; Kheiri, A. (2005) Some species of order Tylenchida collected from alfalfa fields in Hamadan Province. <em>Iranian Journal of Plant Pathology<\/em>, 41, 257\u2013262. [663\u2013685] [In Persian with English summary]<\/p><p class=\"HeadingRunIn\">Holterman, M., Van Der Wurff, A., Van Den Elsen, S., Van Megen, H., Bongers, T., Holovachov, O., Bakker, J. &amp; Helder, J. (2006) Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. <em>Molecular Biology and Evolution,<\/em> 23, 1792\u20131800.<br \/> http:\/\/dx.doi.org\/10.1093\/molbev\/msl044<\/p><p class=\"HeadingRunIn\">Holterman, M., Rybarczyk, K., Van Den Elsen, S., Van Megen, H., Mooyman, P., Pe\u00f1a-Santiago, R., Bongers, T., Bakker, J. &amp; Helder, J. (2008) A ribosomal DNA-based framework for the detection and quantification of stress-sensitive nematode families in terrestrial habitats. <em>Molecular Ecology Resources,<\/em> 8, 23\u201334.<br \/> http:\/\/dx.doi.org\/10.1111\/j.1471-8286.2007.01963.x<\/p><p class=\"HeadingRunIn\">Holterman, M., Karssen, G., van den Elsen, S., van Megen, H., Bakker, J. &amp; Helder, J. (2009) Small subunit rDNA-based phylogeny of the Tylenchida sheds light on relationships among some high-impact plant-parasitic nematodes and the evolution of plant feeding. <em>Phytophathology<\/em>, 99, 227\u2013235.<\/p><p class=\"HeadingRunIn\"> http:\/\/dx.doi.org\/10.1094\/phyto-99-3-0227<\/p><p class=\"HeadingRunIn\">Jenkins, W.R. (1964) A rapid centrifugal-flotation technique for separating nematodes from soil. <em>Plant Disease Reporter<\/em>, 48, 692\u2013692.<\/p><p class=\"HeadingRunIn\">Loof, P.A.A. (1991) The family Pratylenchidae Thorne, 1949. <em>In<\/em>: Nickle, W.R. (Ed.), <em>Manual of Agricultural Nematology<\/em>. New York, USA, CRC Press, pp. 363\u2013421.<\/p><p class=\"HeadingRunIn\">Majd Taheri, Z., Tanha Maafi, Z., Subbotin, S.A., Pourjam, E. &amp; Eskandari, A. (2013) Molecular and phylogenetic studies on Pratylenchidae from Iran with additional data on <em>Pratylenchus delattrei<\/em>, <em>Pratylenchoides alkani<\/em> and two unknown species of <em>Hirschmanniella<\/em> and <em>Pratylenchus<\/em>. <em>Nematology<\/em>, 15, 1\u201319.<\/p><p class=\"HeadingRunIn\"> <a href=\"http:\/\/dx.doi.org\/10.1163\/15685411-00002707\">http:\/\/dx.doi.org\/10.1163\/15685411-00002707.<\/a><\/p><p class=\"HeadingRunIn\">Nunn, G. (1992) <em>Nematode Molecular Evolution.<\/em> Ph.D. thesis. University of Nottingham, U.K., 228 pp.<\/p><p class=\"HeadingRunIn\">Niknam, Gh. &amp; Kheiri, A. (1997) Identification of plant parasitic nematodes (Tylenchida) of Moghan Agrobusiness Corporation farms. <em>Agricultural Science<\/em>, 7 (1), 32, 1\u201332. [In Persian with English summary]<\/p><p class=\"HeadingRunIn\">Panahandeh, Y., Pourjam, E., Atighi, M.R. &amp; Pedram, M. (2014) Morphological and molecular characterization of three species of the genus <em>Pratylenchoides<\/em> Winslow, 1958 (Tylenchina, Merliniidae, Pratylenchoidinae) from Iran. <em>Journal of Crop Protection<\/em>, 3 (supplementary), 691\u2013709.<\/p><p class=\"HeadingRunIn\">Robbins, R.T. (1985) Description of <em>Pratylenchoides sheri<\/em> n. sp. (Nematoda: Pratylenchidae). <em>Journal of Nematology<\/em>, 17, 107\u2013111.<\/p><p class=\"HeadingRunIn\">Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Hohna, S., Larget, B., Liu, L., Suchard, M.A &amp; Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. <em>Systematic Biology,<\/em> 61, 539\u2013542.<br \/> http:\/\/dx.doi.org\/10.1093\/sysbio\/sys029<\/p><p class=\"HeadingRunIn\">Ryss, A.Y. &amp; Sturhan, D. (2001) Three new species of the genus <em>Pratylenchoides<\/em> from Germany (Tylenchida: Pratylenchidae). <em>Zoosystematica Rossica<\/em>, 10, 15\u201331.<\/p><p class=\"HeadingRunIn\">Ryss, A.Y. (1993) Phylogeny of the order Tylenchida (Nematoda). <em>Russian Journal of Nematology<\/em>, 1, 74\u201396.<\/p><p class=\"HeadingRunIn\">Ryss, A.Y. (2007) Taxonomy and evolution of the genus <em>Pratylenchoides<\/em> (Nematoda: Pratylenchidae). <em>Parazitologyia<\/em>, 41, 161\u2013194. [In Russian]<\/p><p class=\"HeadingRunIn\">Sher, S.A. (1970) Revision of the genus <em>Pratylenchoides<\/em> Winslow, 1958 (Nematoda: Tylenchoidea). <em>Proceedings of the Helminthological Society of Washington<\/em>, 37, 154\u2013165.<\/p><p class=\"HeadingRunIn\">Siddiqi, M.R. (1971) On the plant-parasitic nematode genera <em>Histotylenchus<\/em> and <em>Telotylenchoides<\/em> gen. n. (Telotylenchinae), with observations on the genus <em>Paratrophurus<\/em> Arias (Trophurinae). <em>Nematologica<\/em>, 17, 190\u2013200.<br \/> http:\/\/dx.doi.org\/10.1163\/187529271X00035<\/p><p class=\"HeadingRunIn\">Sturhan, D. (2011) On lateral fields and deirids in Merliniinae (Tylenchida, Telotylenchidae) and the genus <em>Pratylenchoides<\/em> (Pratylenchidae). <em>Journal of Nematode Morphology and Systematics<\/em>, 14, 179\u2013182.<\/p><p class=\"HeadingRunIn\">Sturhan, D. (2012) Contribution to a revision of the family Merliniidae Ryss, 1998, with proposal of Pratylenchoidinae subfam. n., <em>Paramerlinius<\/em> gen. n., <em>Macrotylenchus<\/em> gen. n. and description of <em>M. hylophilus<\/em> sp. n. (Tylenchida). <em>Journal of Nematode Morphology and Systematics<\/em>, 15, 127\u2013147.<\/p><p class=\"HeadingRunIn\">Talavera, M. &amp; Tobar, A. (1996) Description of <em>Pratylenchoides nevadensis<\/em> sp. n. from southern Spain (Tylenchida: Pratylenchidae). <em>Afro-Asian Journal of Nematology<\/em>, 6, 46\u201349.<\/p><p class=\"HeadingRunIn\">van Megen, H., van den Elsen, S., Holterman, M., Karssen, G., Mooyman, P., Bongers, T., Holovachov, O., Bakker, J. &amp; Helder, J. (2009) A phylogenetic tree of nematodes based on about 1200 full-length small subunit ribosomal DNA sequences. <em>Nematology<\/em>, 11, 927\u2013950.<br \/> <a href=\"http:\/\/dx.doi.org\/10.1163\/156854109x456862\">http:\/\/dx.doi.org\/10.1163\/ 156854109x456862<\/a><\/p><p class=\"HeadingRunIn\">Whitehead, A.G. &amp; Hemming, J.R. (1965) A comparision of some quantitive methods of extracting vermiform nematodes from soil. <em>Annals of Applied Biology<\/em>, 55, 25\u201338.<br \/> http:\/\/dx.doi.org\/10.1111\/j.1744-7348.1965.tb07864.x<\/p><p class=\"HeadingRunIn\">Winslow, R.D. (1958) The taxonomic position of <em>Anguillulina obtusa <\/em>Goodey, 1932 and1940. <em>Nematologica<\/em>, 3, 136\u2013139.<br \/> http:\/\/dx.doi.org\/10.1163\/187529258X00210<\/p><p class=\"HeadingRunIn\">Yoder, M., Tandingan De Ley, I., King, I.W., Mundo-Ocampo, M., Mann, J., Blaxter, M., Poiras, L. &amp; De Ley, P. (2006) DESS: a versatile solution for preserving morphology and extractable DNA of nematodes. <em>Nematology<\/em>, 8 (3), 367\u2013376.<br \/> http:\/\/dx.doi.org\/10.1163\/156854106778493448<\/p><p> <\/p>"}],"container-title":["Zootaxa"],"original-title":[],"link":[{"URL":"https:\/\/www.mapress.com\/zt\/article\/download\/zootaxa.4205.5.4\/9621","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.mapress.com\/zt\/article\/download\/zootaxa.4205.5.4\/9621","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T23:29:14Z","timestamp":1691105354000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mapress.com\/zt\/article\/view\/zootaxa.4205.5.4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,9]]},"references-count":1,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2016,12,9]]}},"URL":"https:\/\/doi.org\/10.11646\/zootaxa.4205.5.4","relation":{},"ISSN":["1175-5334","1175-5326"],"issn-type":[{"type":"electronic","value":"1175-5334"},{"type":"print","value":"1175-5326"}],"subject":[],"published":{"date-parts":[[2016,12,9]]}}}