{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:35:01Z","timestamp":1777455301387,"version":"3.51.4"},"reference-count":1,"publisher":"Magnolia Press","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zootaxa"],"abstract":"Sheath nematodes of the genus Hemicycliophora are migratory root-ectoparasites of many crops, but the damage is documented for only a few species. Hemicycliophora subbotini n. sp., isolated in Hangzhou, Zhejiang, China, from the rhizosphere of camphor tree (Cinnamomum camphora (L.) Presl), is described and characterized morphologically and molecularly with important morphological details elucidated by SEM studies. The new species can be characterized by the conoid lip region, with bulging oral disc protruding beyond the first annulus in some specimens. Oral disc narrow ovate, amphidial apertures covered by lateral plates, lying in the same plane as oral disc when observed en face. Stylet 118 (108\u2013125) \u03bcm long and flexible, stylet knobs concave shaped having a slight cavity. The excretory pore is located 1\u20132 annuli posterior to the base of pharyngeal bulb. Vulval lips slightly modified with small vulval sleeve, spermatheca rounded to oblong. Anus located 18 (15\u201322) annuli posterior to vulva. Tail elongated, with conoid or slightly offset spike having a narrowly rounded terminus. The study provides the first description of a new Hemicycliophora species from China. Phylogenetic analyses based on D2\u2013D3 expansion segments of the 28S and ITS fragments revealed the new species forming a separate clade from other Hemicycliophora species which supported the status as a new species of this genus.\u00a0<\/jats:p>","DOI":"10.11646\/zootaxa.4433.1.10","type":"journal-article","created":{"date-parts":[[2018,6,11]],"date-time":"2018-06-11T00:58:40Z","timestamp":1528678720000},"source":"Crossref","is-referenced-by-count":3,"title":["Morphological and molecular characterization of Hemicycliophora subbotini n. sp. (Tylenchida: Hemicycliophoridae) from China"],"prefix":"10.11646","volume":"4433","author":[{"given":"MUNAWAR","family":"MARIA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RUIHANG","family":"CAI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"NAN","family":"QU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PABLO","family":"CASTILLO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JINGWU","family":"ZHENG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4913","published-online":{"date-parts":[[2018,6,11]]},"reference":[{"key":"42934","unstructured":"

Castresana, J. (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution<\/em>, <\/em>17, 540\u2013552.
https:\/\/doi.org\/10.1093\/oxfordjournals.molbev.a026334<\/p>

Chen, H.P., Yang, K., You C.X., Lei, N., Sun, R.Q., Geng, Z.F., Ma, P., Cai, Q., Du, S.S. & Deng, Z.W. (2014) Chemical constituents and insecticidal activities of the essential oil of Cinnamomum camphora<\/em> leaves against Lasioderma serricorne<\/em>. Journal of Chemistry<\/em>, 2014, 1\u20135.
https:\/\/doi.org\/10.1155\/2014\/963729<\/p>

Chitambar, J.J & Subbotin, S.A. (2014) Systematics of the sheath nematodes of the superfamily Hemicycliophoroidea. In<\/em>: Nematology <\/em>Monograph and Perspectives. Vol. 10<\/em>. Brill Publishers, Leiden, pp. 1\u2013732.<\/p>

Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature <\/em>Methods, 9, 772.
https:\/\/doi.org\/10.1038\/nmeth.2109<\/p>

De Grisse, A.T. (1969) Redescription ou modification de quelques techniques utilis\u00e9es dans l\u2019\u00e9tude des nematodes phytoparasitaires. Mededelingen van de Rijksfaculteit der<\/em> Landbouwwetenschappen Gent<\/em>, 34, 351\u2013369.<\/p>

De Ley, P., F\u00e9lix, M.A., Frisse, L.M., Nadler, S.A., Sternberg, P.W. & Thomas, W.K. (1999) Molecular and morphological characterization of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology<\/em>, 1, 591\u2013612.
https:\/\/doi.org\/10.1163\/156854199508559<\/p>

Doucet, M.E. (1982) Quatre nouvelles especes du genre Hemicycliophora <\/em>De Man, 1921 (Nematoda: Tylenchida) provenant d\u2019Argentine. Revue de N\u00e9matologie<\/em>, <\/em>5, 309\u2013320.<\/p>

Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98NT. Nucleic Acids Symposium Serial<\/em>, 41, 95\u201398.<\/p>

Jenkins, W.R. (1964) A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Disease Reporters<\/em>, <\/em>48, 692.<\/p>

Jenkins, W.R., & Reed, J.P. (1964) Two new species of Hemicycliophora<\/em> (Nematoda: Criconematidae) with a note on Hemicycliophora ritteri<\/em>. Nematologica<\/em>, 10, 111\u2013115.
https:\/\/doi.org\/10.1163\/187529264X00718<\/p>

Joyce, S., Reid, A., Driver, F. & Curran, J. (1994) Application of polymerase chain reaction (PCR) methods to identification of entomopathogenic nematodes. In<\/em>: Burnell, A.M., Ehlers, R.U. & Masson, J.P. (Eds.), COST 812 Biotechnology<\/em>: Genetics of Entomopathogenic Nematode-bacterium Complexes Proceedings of Symposium & Workshop<\/em>, St. Patrick\u2019s College, Maynooth, County Kildare and European Commission, DG, Luxembourg, pp. XII + 178\u2013187.<\/p>

Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment 542 software version 7: improvements in performance and usability. Molecular Biology and Evolution<\/em>, 30, 772\u2013780.
https:\/\/doi.org\/10.1093\/molbev\/mst010<\/p>

Li, X.W., Li, J. & Van der Werff, A. (2008) Cinnamomum Schaeffer. In<\/em>: Wu, Z.Y., Raven, P. (Eds.), Flora of China. Vol. 7<\/em>. Science Press, St. Louis, pp. 166\u2013187.<\/p>

Loof, P.A.A. (1968) Taxonomy of Hemicycliophora <\/em>species from west and central Europe (Nematoda: Criconematoidea). Mededelingen Landbouwhogeschool Wageningen<\/em>, <\/em>68, 1\u201343.<\/p>

Man, J.G. de (1921) Nouvelles recherches sur les n\u00e9matodes libres terricoles de la Hollande. Capita Zoologica<\/em>, <\/em>1, 3\u201362.<\/p>

Monteiro, A.R. & Lordello, L.G.E. (1978) A description of Hemicycliophora poranga <\/em>n. sp. from Brazil (Nemata). Revista Brasileira de Biologia<\/em>, 38, 569\u2013571.<\/p>

Maria, M., Cai, R., Castillo, P. & Zheng, J. (2018) Morphological and molecular characterization of Hemicriconemoides paracamelliae<\/em> sp. n. (Nematoda: Criconematidae) and two known species of Hemicriconemoides<\/em> from China. Nematology<\/em>, 20. [published online]<\/p>

https:\/\/doi.org\/ 10.1163\/15685411-00003147<\/p>

Olson, M., Harris, T., Higgins, R., Mullin, P., Powers, K., Olson, S. & Powers, T.O. (2017) Species delimitation and description of Mesocriconema nebraskense<\/em> n. sp. (Nematoda: Criconematidae), a morphologically cryptic, parthenogenetic species from North American Grasslands. Journal of Nematology<\/em>, 49, 42\u201366.
https:\/\/doi.org\/10.21307\/jofnem-2017-045<\/p>

Powers, T.O., Harris, T., Higgins, R., Sutton, L. & Powers, K.S. (2010) Morphological and molecular characterization of Discocriconemella inarata<\/em>, an endemic nematode from North American native tallgrass prairies. Journal of Nematology<\/em>, 42, 35\u201345.<\/p>

Raski, D.J. (1958) Four new species of Hemicycliophora<\/em> de Man, 1921, with further observations on H. brevis<\/em> Thorne, 1955 (Nematoda: Criconematidae). Proceedings of the Helminthological Society of Washington<\/em>, 25, 125\u2013131.<\/p>

Reed, J.P. & Jenkins, W.R. (1963) Hemicycliophora vaccinium<\/em> n. sp. (Nematoda: Criconematidae) from cranberry. Proceedings of the Helminthological Society of Washington<\/em>, <\/em>30, 211\u2013212.<\/p>

Ronquist, F. & Huelsenbeck, J.P. (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics<\/em>, 19, 1572\u20131574.
https:\/\/doi.org\/10.1093\/bioinformatics\/btg180<\/p>

Seinhorst, J.W. (1959) A rapid method for the transfer of nematodes from \ufb01xative to anhydrous glycerin. Nematologica<\/em>, 4, 67\u201369.
https:\/\/doi.org\/10.1163\/187529259X00381<\/p>

Shi, Y., Chen, F., Ye, J, Fang, G. & Pan, H. (2012) Classification of parasitic nematodes in pine trees in China. Journal of Nanjing Forestry University<\/em>, 36, 137\u2013141. [in Chinese]<\/p>

Siddiqi M.R. (2000) Tylenchida parasites of plants and insects.<\/em> 2<\/em>nd<\/sup> Edition. CABI Publishing Wallingford, 848 pp.
https:\/\/doi.org\/10.1079\/9780851992020.0000<\/p>

Subbotin, S.A., Chitambar, J.J., Chizhov, V.N., Stanley, J.D., Inserra, R.N., Doucet, M.E., McClure, M., Ye, W., Yeates, G.W., Mollov, D.S., Cantalapiedra-Navarrete, C., Vovlas, N., Van Den Berg, E. & Castillo, P. (2014) Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora <\/em>(Nematoda: Hemicycliophoridae). Zoological Journal of the Linnean Society<\/em>, <\/em>171, 475\u2013506.
https:\/\/doi.org\/10.1111\/zoj.12145<\/p>

Tarjan, A.C. (1952) The nematode genus Hemicycliophora <\/em>De Man, 1921 (Criconematidae) with a description of a new plant-parasitic species. Proceedings of the Helminthological Society of Washington<\/em>, 19, 65\u201377.<\/p>

Thorne, G. (1955) Fifteen new species of the genus Hemicycliophora <\/em>with an amended description of H. typical<\/em> de Man (Tylenchida Criconematidae). Proceedings of the
Helminthological Society of Washington<\/em>, <\/em>22, 1\u201316.<\/p>

Van den Berg, E. (1981) Further studies on the genus Hemicycliophora<\/em> de Man, 1921 in South Africa (Nematoda: Hemicycliophoroidea) with description of a new species. Phytophylactica<\/em>, 13, 181\u2013194.<\/p>

Van den Berg, E., Tiedt, L.R., Liebanas, G., Chitambar, J.J., Stanley, J.D., Inserra, R.N., Castillo, P. & Subbotin, S.A. (2018) Morphological and molecular characterization of two new Hemicycliophora <\/em>species (Tylenchida: Hemicycliophoridae) with a revision of the taxonomic status of some known species and a phylogeny of the genus. Nematology<\/em>, 20, 319\u2013354.
https:\/\/doi.org\/10.1163\/15685411-00003143<\/p>

Ye, W., Giblin-Davis, R.M., Braasch, H., Morris, K. & Thomas, W.K. (2007) Phylogenetic relationships among Bursaphelenchus<\/em> species (Nematoda: Parasitaphelenchidae) inferred from nuclear ribosomal and mitochondrial DNA sequence data. Molecular Phylogenetics and Evolution<\/em>, 43, 1185\u20131197.
https:\/\/doi.org\/10.1016\/j.ympev.2007.02.006<\/p>

Zhang, X.K., Liang, W.J. Jiang, D.M., Liu, Z.M. & Jiang S. (2007). W. Soil nematode community structure in a Chinese sand dune system. Helminthologica<\/em>, 44, 204\u2013209. [in Chinese]
https:\/\/doi.org\/10.2478\/s11687-007-0032-6<\/p>

Zheng, J., Subbotin, S.A., He, S., Gu, J. & Moens, M. (2003) Molecular characterization of some Asian isolates of Bursaphelenchus xylophilus<\/em> and B. mucronatus<\/em> using PCR-RFLPs and sequences of ribosomal DNA. Russian Journal of Nematology<\/em>, 11, 17\u201322.<\/p>

<\/p>

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