{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,7]],"date-time":"2024-07-07T12:29:57Z","timestamp":1720355397830},"reference-count":1,"publisher":"Magnolia Press","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Zootaxa"],"abstract":"Two new species of Tubificoides (subfamily Tubificinae), T. charlotteae n. sp. and T. mackiei n. sp., are described based on morphological analyses. Both species were flagged as potentially cryptic in a previous investigation, based on both mitochondrial and nuclear sequence data. Tubificoides charlotteae n. sp., known only from an intertidal site in southern Spain, is characterized by the lack of cuticular papillation, the possession of several needle-like hair chaetae in dorsal bundles, a cone-shaped penis sheath, and a rather large, muscular penial sac. It strongly resembles the north-west European, largely sublittoral species T. amplivasatus, but differs from this species in terms of body size, width of vas deferens, and the shape and size of both the ejaculatory duct and penial sac. Tubificoides mackiei n. sp., collected from the southeast coast of Norway and the west coast of Sweden, is characterized by the lack of cuticular papillae and hair chaetae, and the possession of a rather long penis sheath with a wide terminal opening. It shares several morphological features with the sympatric species Tubificoides pseudogaster, but is distinguished from the latter by the detailed morphology and length of the penis sheaths, the width of the atrium, and the lower maximal number of bifid chaetae in dorsal preclitellar bundles. The utility of an integrative comparative approach, combining molecules and morphology, for the identification and delineation of new taxa within Tubificoides is briefly discussed.\u00a0<\/jats:p>","DOI":"10.11646\/zootaxa.4433.3.10","type":"journal-article","created":{"date-parts":[[2018,6,13]],"date-time":"2018-06-13T01:28:51Z","timestamp":1528853331000},"source":"Crossref","is-referenced-by-count":1,"title":["Two new European species of the marine genus Tubificoides (Annelida: Clitellata: Naididae) with notes on the morphology of T. pseudogaster (Dahl, 1960)"],"prefix":"10.11646","volume":"4433","author":[{"given":"SEBASTIAN","family":"KVIST","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CHRISTER","family":"ERS\u00c9US","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4913","published-online":{"date-parts":[[2018,6,13]]},"reference":[{"key":"42960","unstructured":"

Achurra, A., Creuze de Chatelliers, M. & Rodriguez, P. (2012) Troglodrilus jugeti <\/em>n. sp. (Annelida, Clitellata, Tubificinae), a new stygobiont oligochaete species from south-western Europe. Zootaxa<\/em>, 3229, 35\u201346.<\/p>

Achurra, A., Rodriguez, P. & Ers\u00e9us, C. (2015) Pseudo-cryptic speciation in the subterranean medium: a new species of Stylodrilus <\/em>Clapar\u00e8de, 1862, with a revision of the status of Bichaeta <\/em>Bretscher, 1900 (Annelida, Clitellata, Lumbriculidae). Zoologischer Anzeiger<\/em>, 257, 71\u201386.
https:\/\/doi.org\/10.1016\/j.jcz.2015.05.003<\/p>

Baker, H.R. (1980) A redescription of Tubificoides pseudogaster<\/em> (Dahl) (Oligochaeta: Tubificidae). Transactions of the American Microscopical Society<\/em>, 99 (3), 337\u2013342.
https:\/\/doi.org\/10.2307\/3226011<\/p>

Baker, H.R. (1983) New species of Tubificoides <\/em>Lastockin (Oligochaeta; Tubificidae) from the Pacific Northeast and the Arctic. Canadian Journal of Zoology<\/em>, 61 (6), 1270\u20131283.
https:\/\/doi.org\/10.1139\/z83-171<\/p>

Baker, H.R. (1984) Diversity and zoogeography of marine Tubificidae (Annelida, Oligochaeta) with notes on variation in widespread species. Hydrobiologia<\/em>, 115 (1), 191\u2013196.
https:\/\/doi.org\/10.1007\/BF00027916<\/p>

Bamber, R.N. & Spencer, J.F. (1984) The benthos of a coastal power station thermal discharge canal. Journal of the Marine Biological Association of the UK<\/em>, 64 (3), 603\u2013623.
https:\/\/doi.org\/10.1017\/S0025315400030290<\/p>

Bickford, D., Lohman, D.J., Sodhi, N.S., Ng, P.K., Meier, R., Winker, K., Ingra, K.K. & Das, I. (2007) Cryptic species as a window on diversity and conservation. Trends in Ecology and Evolution<\/em>, 22 (3), 148\u2013155.
https:\/\/doi.org\/10.1016\/j.tree.2006.11.004<\/p>

Brinkhurst, R.O. (1981) A contribution to the taxonomy of the Tubificinae (Oligochaeta: Tubificidae). Proceedings of the Biological Society of Washington<\/em>, 94 (4), 1048\u20131067.<\/p>

Brinkhurst, R.O. (1985) A further contribution to the taxonomy of the genus Tubificoides<\/em> Lastockin (Oligochaeta: Tubificidae). Canadian Journal of Zoology<\/em>, 63 (2), 400\u2013410.
https:\/\/doi.org\/10.1139\/z85-061<\/p>

Brinkhurst, R.O. (1986) Taxonomy of the genus Tubificoides<\/em> Lastockin (Oligochaeta, Tubificidae): species with bifid setae. Canadian Journal of Zoology<\/em>, <\/em>64 (6), 1270\u20131279.
https:\/\/doi.org\/10.1139\/z86-189<\/p>

Brinkhurst, R.O. & Baker, H.R. (1979) A review of the marine Tubificidae (Oligochaeta) of North America. Canadian Journal of Zoology<\/em>, 57 (8), 1553\u20131569.
https:\/\/doi.org\/10.1139\/z79-203<\/p>

Buckley, T.R., James, S., Allwood, J., Bartlam, S., Howitt, R. & Prada, D. (2011) Phylogenetic analysis of New Zealand earthworms (Oligochaeta: Megascolecidae) reveals ancient clades and cryptic taxonomic diversity. Molecular Phylogenetics and Evolution<\/em>, 58 (1), 85\u201396.
https:\/\/doi.org\/10.1016\/j.ympev.2010.09.024<\/p>

Chang, C.-H., Lin, S.-M. & Chen, J.-H. (2008) Molecular systematics and phylogeography of the gigantic earthworms of the Metaphire formosae <\/em>species group (Clitellata, Megascolecidae). Molecular Phylogenetics and Evolution<\/em>, 49 (3), 958\u2013968.
https:\/\/doi.org\/10.1016\/j.ympev.2008.08.025<\/p>

Clapar\u00e8de, E.R. (1862) Recherches anatomiques sur les oligochetes. Memoirs of the Society of Physics and Natural History Geneva<\/em>, 16, 217\u2013291.<\/p>

Cook, D.G. (1969) Peloscolex dukei<\/em> n.sp. and P. aculeatus<\/em> n.sp. (Oligochaeta, Tubificidae) from the North-west Atlantic, the latter being from abyssal depths. Transactions of the American Microscopical Society<\/em>, 88 (4), 492\u2013497.
https:\/\/doi.org\/10.2307\/3224243<\/p>

Cook, D.G. (1970) Bathyal and abyssal Tubificidae (Annelida, Oligochaeta) from the Gay Head\u2013Bermuda transect, with descriptions of new genera and species. Deep\u2013Sea Research<\/em>, 17 (6), 973\u2013981.
https:\/\/doi.org\/10.1016\/0011-7471(70)90048-3<\/p>

Dahl, I.O. (1960) The oligochaete fauna of 3 Danish brackish water areas (taxonomic and biological observations). Meddelelser fra Danmarks Fiskeri- og Havunders\u00f8gelser<\/em>, New Series, 26, 1\u201320.<\/p>

De Wit, P. & Ers\u00e9us, C. (2010) Genetic variation and phylogeny of Scandinavian species of Grania <\/em>(Annelida: Clitellata: Enchytraeidae), with the discovery of a cryptic species. Journal of Zoological Systematics and Evolutionary Research<\/em>, 48 (4), 285\u2013293.
https:\/\/doi.org\/10.1111\/j.1439-0469.2010.00571.x<\/p>

De Queiroz, K. (2007) Species concepts and species delimitation. Systematic Biology<\/em>, 56 (6), 879\u2013886.
https:\/\/doi.org\/10.1080\/10635150701701083<\/p>

Donnelly, R.K., Harper, G.L., Morgan, A.J., Orozco-Terwengel, P., Pinto-Juma, G.A. & Bruford, M.W. (2013) Nuclear DNA recapitulates the cryptic mitochondrial lineages of Lumbricus rubellus <\/em>and suggests the existence of cryptic species in an ecotoxicological soil sentinel. Biological Journal of the Linnean Society<\/em>, 110 (4), 780\u2013795.
https:\/\/doi.org\/10.1111\/bij.12171<\/p>

d\u2019Udekem, J. (1855) Histoire naturelle du Tubifex des ruisseaux. M\u00e9moires Couronn\u00e9s des Savantes Etrangers de l\u2019Acad\u00e9mie Royal de Belgique<\/em>, 26, 1\u201338.<\/p>

Ers\u00e9us, C. (1975) Peloscolex amplivasatus <\/em>sp.n. and Macroseta rarisetis <\/em>gen. et sp.n. (Oligochaeta, Tubificidae) from the West coast of <\/em>Norway. Sarsia<\/em>, 58 (1), 1\u20138.
https:\/\/doi.org\/10.1080\/00364827.1975.10411274<\/p>

Ers\u00e9us, C. (1976) Marine subtidal Tubificidae and Enchytraeidae (Oligochaeta) of the Bergen area, Western Norway. Sarsia<\/em>, 62 (1), 25\u201348.
https:\/\/doi.org\/10.1080\/00364827.1976.10411312<\/p>

Ers\u00e9us, C. & Gustafsson, D. (2009) Cryptic speciation in clitellate model organisms. In<\/em>: Shain, D. (Ed.), Annelids in Modern Biology<\/em>. <\/em>John Wiley & Sons, Hoboken, New Jersey, pp. 31\u201346.
https:\/\/doi.org\/10.1002\/9780470455203.ch3<\/p>

Ers\u00e9us, C. & Kvist, S. (2007) COI variation in Scandinavian marine species of Tubificoides<\/em> (Annelida: Clitellata: Tubificidae). Journal of the Marine Biological Association of the UK<\/em>, <\/em>87 (5), 1121\u20131126.
https:\/\/doi.org\/10.1017\/S0025315407055610<\/p>

Ers\u00e9us, C., Wetzel, M.J. & Gustavsson, L. (2008) ICZN rules\u2014a farewell to Tubificidae (Annelida, Clitellata). Zootaxa<\/em>, 1744 (1), 66\u201368.
https:\/\/doi.org\/10.11646\/zootaxa.1744.1.7<\/p>

Gustafsson, D.R., Price, D.A. & Ers\u00e9us, C. (2009) Genetic variation in the popular lab worm Lumbriculus variegatus<\/em> (Annelida: Clitellata: Lumbriculidae) reveals cryptic speciation. Molecular Phylogenetics and Evolution<\/em>, 51 (2), 182\u2013189.
https:\/\/doi.org\/10.1016\/j.ympev.2008.12.016<\/p>

Hebert, P.D.N., Penton E.H., Burns, J.M., Janzen, D.H. & Hallwachs, W. (2004) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator.<\/em> Proceedings of the National Academy of Sciences USA<\/em>, 101 (41), 14812\u201314817.
https:\/\/doi.org\/10.1073\/pnas.0406166101<\/p>

Hebert, P.D.N., Ratnasingham, S. & deWaard, J.R. (2003) Barcoding animal life: cytochrome c<\/em> oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society of London<\/em>, Series B (Biological Sciences), 270 (1512), 96\u201399. [S96\u2013S99]
https:\/\/doi.org\/10.1098\/rsbl.2003.0025<\/p>

Helgason, G.V. & Ers\u00e9us, C. (1987) Three new species of Tubificoides<\/em> (Oligochaeta, Tubificidae) from the North\u2013west Atlantic and notes on geographic variation in the circumpolar T. kozloffi.<\/em> Sarsia<\/em>, 72 (2), 159\u2013169.
https:\/\/doi.org\/10.1080\/00364827.1987.10419713<\/p>

Holmquist, C. (1978) Revision of the genus Peloscolex<\/em> (Oligochaeta, Tubificidae). 1. Morphological and anatomical scrutiny; with discussion on the generic level. Zoologica Scripta<\/em>, 7, 187\u2013209.
https:\/\/doi.org\/10.1111\/j.1463-6409.1978.tb00602.x<\/p>

Holmquist, C. (1979) Revision of the genus Peloscolex<\/em> (Oligochaeta, Tubificidae). 2. Scrutiny of the species. Zoologica Scripta<\/em>, 8, 37\u201360.
https:\/\/doi.org\/10.1111\/j.1463-6409.1979.tb00618.x<\/p>

Hrab\u011b, S. (1966) New or insufficiently known species of the family Tubificidae. Publicationes Facultatis Scientarium Universitatis J. E. Purkyn\u011b, Brno<\/em>, 470, 57\u201376.<\/p>

James, S. W., Porco, D., Decaens, T., Richard, B., Rougerie, R. & Ers\u00e9us, C. (2010) DNA barcoding reveals cryptic diversity in Lumbricus terrestris <\/em>L., 1758 (Clitellata): resurrection of L. herculeus <\/em>(Savigny, 1826). PLoS ONE<\/em>, 5, e15629.
https:\/\/doi.org\/10.1371\/journal.pone.0015629<\/p>

Juget, J., des Chatelliers, M. & Rodriguez, P. (2006) Troglodrilus<\/em> (Annelida, Oligochaeta, Tubificidae), a new genus from subterranean habitats in southwestern Europe. Hydrobiologia<\/em>, 564, 7\u201317.
https:\/\/doi.org\/10.1007\/s10750-005-1703-2<\/p>

Kawauchi, G.Y. & Giribet, G. (2014) Sipunculus nudus <\/em>Linnaeus, 1766 (Sipuncula): cosmopolitan or a group of pseudo-cryptic species? An integrated molecular and morphological approach. Marine Ecology<\/em>, 35 (4), 478\u2013491.
https:\/\/doi.org\/10.1111\/maec.12104<\/p>

Kvist, S., Dreyer, J. & Ers\u00e9us, C. (2008) Two new species of Tubificoides<\/em> (Annelida: Clitellata: Naididae) from the Blake Ridge methane seep in the North\u2013west Atlantic Ocean. Proceedings of the Biological Society of Washington B<\/em>, 121 (4), 531\u2013540.
https:\/\/doi.org\/10.2988\/08-14.1<\/p>

Kvist, S., Sarkar, I.N. & Ers\u00e9us, C. (2010) Genetic variation and phylogeny of the cosmopolitan marine genus Tubificoides<\/em> (Annelida: Clitellata: Naididae: Tubificinae). Molecular Phylogenetics and Evolution<\/em>, 57 (2), 687\u2013702.
https:\/\/doi.org\/10.1016\/j.ympev.2010.08.018<\/p>

Laakso, M. (1969) Oligochaeta from brackish water near Tv\u00e4rminne, south-west Finland. Annales Zoologici Fennici<\/em>, <\/em>6 (1), 98\u2013111.<\/p>

Lamarck, J.B. (1816) Histoire naturelle des animaux sans vert\u00e8bres 3<\/em>. Verdi\u00e8re, Paris, 585 pp.<\/p>

Lastockin, D.A. (1937) New species of Oligochaeta limicola in the European part of the U.S.S.R. Compte Rendus (Doklady) de l\u2019Acad\u00e9mie des Sceinces de l\u2019URSS<\/em>, 17, 233\u2013235.<\/em><\/p>

Leidy, J. (1851) Description of new genera of Vermes. Proceedings of the Academy of Natural Sciences of Philadelphia<\/em>, 5, 239\u2013244.<\/p>

Liu, Y., Fend, S.V., Martinsson, S. & Ers\u00e9us, C. (2017) Extensive cryptic diversity in the cosmopolitan sludge worm Limnodrilus hoffmeisteri<\/em> (Clitellata, Naididae). Organisms Diversity & Evolution<\/em>, 17 (2), 477\u2013495.
https:\/\/doi.org\/10.1007\/s13127-016-0317-z<\/p>

Martinsson, S., Achurra, A., Svensson, M. & Ers\u00e9us, C. (2013) Integrative taxonomy of the freshwater worm Rhyacodrilus falciformis<\/em> s.l. (Clitellata: Naididae), with the description of a new species. Zoologica Scripta<\/em>, 42 (6), 612\u2013622.<\/p>

Martinsson, S. & Ers\u00e9us, C. (2014) Cryptic diversity in the well\u2013studied terrestrial worm Cognettia sphagnetorum <\/em>(Clitellata: Enchytraeidae). Pedobiologia<\/em>, 57 (1), 27\u201335.
https:\/\/doi.org\/10.1016\/j.pedobi.2013.09.006<\/p>

Martinsson, S. & Ers\u00e9us, C. (2017) Cryptic speciation and limited hybridization within Lumbricus<\/em> earthworms (Clitellata: Lumbricidae). Molecular Phylogenetics and Evolution<\/em>, 106 (2017), 18\u201327.
https:\/\/doi.org\/10.1016\/j.ympev.2016.09.011<\/p>

Matamoros, L., Rota, E. & Ers\u00e9us, C. (2012) Cryptic diversity among the achaetous Marionina <\/em>(Annelida, Clitellata, Enchytraeidae). Systematics and Biodiversity<\/em>, 10 (4), 509\u2013525.
https:\/\/doi.org\/10.1080\/14772000.2012.723640<\/p>

Michaelsen, W. (1926) Oligochaeten aus dem Ryck bei Greifswald und von benachbarten Meeresgebieten. Mitteilungen aus den Hamburgischen Zoologischen Museum und Institut<\/em>, 42, 21\u201329.<\/p>

Milligan, M.R. (1996) Identification manual for the aquatic Oligochaeta of Florida. Volume II. Estuarine and nearshore marine oligochaetes. Final Report for DEP Contract Number WM550.<\/em> Florida Department of Environmental Protection, Division of Water Facilities, Tallahassee, Florida, 239 pp.<\/p>

Moura, C.J., Harris, D.J., Cunha, M.R. & Rogers, A.D. (2008) DNA barcoding reveals cryptic diversity in marine hydroids (Cnidaria, Hydrozoa) from coastal environments. Zoologica Scripta<\/em>, 37 (1), 93\u2013108.<\/p>

Nygren, A. (2014) Cryptic polychaete diversity: a review. Zoologica Scripta<\/em>, 43 (2), 172\u2013183.
https:\/\/doi.org\/10.1111\/zsc.12044<\/p>

Ratnasingham, S. & Hebert, P.D.N. (2007) BOLD: the barcode of life data system. Molecular Ecology Resources<\/em>, 7 (3), 355\u2013364.
https:\/\/doi.org\/10.1111\/j.1471-8286.2007.01678.x<\/p>

Reise, K., Herre, E, & Sturm, M. (1994) Biomass and abundance of macrofauna in intertidal sediments of K\u00f6nigshafen in the northern Wadden Sea. Helgol\u00e4nder Meeresuntersuchungen<\/em>, 48, 201\u2013215.
https:\/\/doi.org\/10.1007\/BF02367036<\/p>

Sarkar, I.N., Planet, P.J., Bael, T.E., Stanley, S.E., Siddall, M., DeSalle, R. & Figurski, D.H. (2002) Characteristic attributes in cancer microarrays. Journal of Biomedical Informatics<\/em>, 35 (2), 111\u2013122.
https:\/\/doi.org\/10.1016\/S1532-0464(02)00504-X<\/a><\/p>

Savigny, J.C. (1822) Syst\u00e8me des ann\u00e9lides, principalement de celles des c\u00f4tes de l\u2019\u00c9gypte et de la Syrie, offrant les caract\u00e8res tant distinctifs que naturels des ordres, familles et genres, avec la description des esp\u00e8ces<\/em>. Description de l\u2019Egypte. Histoire Naturelle, Paris<\/em>, 1, 1\u2013128.<\/p>

Smith, M.A., Fisher, B.L. & Hebert, P.D.N. (2005) DNA barcoding for effective biodiversity assessment of a hyperdiverse arthropod group: the ants of Madagascar. Philosophical Transactions of the Royal Society B: Biological Sciences<\/em>, <\/em>360 (1462), 1825\u20131834.
https:\/\/doi.org\/10.1098\/rstb.2005.1714<\/p>

Takashima, Y. & Mawatari, S.F. (1996) Marine Tubificidae (Oligochaeta) from Hokkaido, northern Japan, with descriptions of two new species. Species Diversity<\/em>, 1, 55\u201370.
https:\/\/doi.org\/10.12782\/specdiv.1.55<\/p>

Witt, J.D.S., Threloff, D.L. & Hebert, P.D.N. (2006) DNA barcoding reveals extraordinary cryptic diversity in an amphipod genus: implications for desert spring conservation. Molecular Ecology<\/em>, 15 (10), 3073\u20133082.
https:\/\/doi.org\/10.1111\/j.1365-294X.2006.02999.x<\/p>

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