{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T03:10:22Z","timestamp":1774667422613,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[1990,6,1]],"date-time":"1990-06-01T00:00:00Z","timestamp":644198400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1990,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Neutrophil attractant\/activation protein-1 (NAP-1 [interleukin-8]) is an 8,400 D protein that is a chemoattractant and granule release stimulus for neutrophils. NAP-1 was first purified from culture fluids of lipopolysaccharide-stimulated human blood mononuclear leukocytes. It was subsequently isolated from lipopolysaccharide-stimulated lung macrophages, mitogen-stimulated lymphocytes, and virus-infected fibroblasts. Interleukin-1 or tumor necrosis factor induces NAP-1 mRNA in many cells, including monocytes, fibroblasts, and endothelial cells. NAP-1 belongs in a family of host defense small proteins, which have a degree of sequence and structural similarity. Noteworthy are the four half-cystine residues in each protein, which are in register when the protein sequences are suitably aligned. Based on cloning data and N-terminal sequence analyses, NAP-1 is secreted as a 79 residue protein after cleavage of a 20 residue signal peptide. The commonly isolated 77 and 72 residue forms are probably extracellular cleavage products. NAP-1 has considerable charge heterogeneity. Charge and length variants all have chemotactic activity. In contrast to many chemoattractants, NAP-1 does not attract monocytes. Intradermal injection of NAP-1 causes neutrophil infiltration. The wide spectrum of cell sources and production stimuli suggests that NAP-1 mediates neutrophil recruitment in host defense and disease.<\/jats:p>","DOI":"10.1165\/ajrcmb\/2.6.479","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T23:13:01Z","timestamp":1365030781000},"page":"479-486","source":"Crossref","is-referenced-by-count":152,"title":["Neutrophil Attractant\/Activation Protein-1 (NAP-1 [Interleukin-8])"],"prefix":"10.1093","volume":"2","author":[{"given":"Edward J.","family":"Leonard","sequence":"first","affiliation":[{"name":"Immunopathology Section, Laboratory of Immunobiology, National Cancer Institute, Frederick, Maryland"}]},{"given":"Teizo","family":"Yoshimura","sequence":"additional","affiliation":[{"name":"Immunopathology Section, Laboratory of Immunobiology, National Cancer Institute, Frederick, Maryland"}]}],"member":"286","published-online":{"date-parts":[[1990,6,1]]},"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/2\/6\/479\/67278314\/ajrcmb_2_6_479.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/2\/6\/479\/67278314\/ajrcmb_2_6_479.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T09:29:50Z","timestamp":1773826190000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/2\/6\/479\/8511944"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[1990,6,1]]},"published-print":{"date-parts":[[1990,6,1]]}},"URL":"https:\/\/doi.org\/10.1165\/ajrcmb\/2.6.479","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1990,6]]},"published":{"date-parts":[[1990,6,1]]}}}