{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:46:43Z","timestamp":1772930803291,"version":"3.50.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1997,7,1]],"date-time":"1997-07-01T00:00:00Z","timestamp":867715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The cytokine transforming growth factor-\u03b2 (TGF-\u03b2) has multiple effects on a wide variety of cell types. These effects include modulation of growth and regulation of gene transcription. In a few instances, TGF-\u03b2 has also been shown to regulate gene expression posttranscriptionally by altering message stability, but the pathway by which this activity is executed remains largely unknown. In the present work, we demonstrate that TGF-\u03b21 has no effect on transcription of the elastin gene in cultured human fetal lung fibroblasts, but does stabilize elastin messenger RNA (mRNA), leading to a dramatic increase in the steady-state level of elastin mRNA. A corresponding increase in production of tropoelastin accompanies the increase in elastin mRNA. Through the use of specific inhibitors, we demonstrate that phosphatidylcholine (PC)-specific phospholipase C (PLC) and protein kinase C (PKC) are involved in mediating the elastin message stabilization. In contrast, G proteins and extracellularly regulated kinases do not appear to be involved. These results suggest that although the TGF-\u03b2 signaling pathway leading to message stabilization shares components with that modulating transcription, the message-stabilization pathway also contains diverse other elements.<\/jats:p>","DOI":"10.1165\/ajrcmb.17.1.2816","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T22:17:51Z","timestamp":1365027471000},"page":"10-16","source":"Crossref","is-referenced-by-count":61,"title":["Stabilization of Elastin mRNA by TGF-\u03b2: Initial Characterization of Signaling Pathway"],"prefix":"10.1093","volume":"17","author":[{"given":"Umberto","family":"Kucich","sequence":"first","affiliation":[{"name":"Department of Anatomy and Histology, University of Pennsylvania School of Dental Medicine, Philadelphia, Pennsylvania"}]},{"given":"Joan C.","family":"Rosenbloom","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology, University of Pennsylvania School of Dental Medicine, Philadelphia, Pennsylvania"}]},{"given":"William R.","family":"Abrams","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology, University of Pennsylvania School of Dental Medicine, Philadelphia, Pennsylvania"}]},{"given":"Muhammad M.","family":"Bashir","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology, University of Pennsylvania School of Dental Medicine, Philadelphia, Pennsylvania"}]},{"given":"Joel","family":"Rosenbloom","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology, University of Pennsylvania School of Dental Medicine, Philadelphia, Pennsylvania"}]}],"member":"286","published-online":{"date-parts":[[1997,7,1]]},"reference":[{"key":"2026030700201118100_B1"},{"key":"2026030700201118100_B2","doi-asserted-by":"crossref","first-page":"6407","DOI":"10.1016\/S0021-9258(18)68800-3","article-title":"Latent high-molecular-weight complex of transforming growth factor-\u03b21: purification from human platelets and structural characterization.","volume":"263","author":"Miyazono","year":"1988","journal-title":"J. Biol. Chem."},{"key":"2026030700201118100_B3","doi-asserted-by":"crossref","first-page":"7646","DOI":"10.1016\/S0021-9258(18)68547-3","article-title":"Latent transforming growth factor-\u03b2 from human platelets.","volume":"263","author":"Wakefield","year":"1988","journal-title":"J. Biol. Chem."},{"key":"2026030700201118100_B4","doi-asserted-by":"crossref","first-page":"19482","DOI":"10.1016\/S0021-9258(18)41801-7","article-title":"Transforming growth factor-\u03b21, -\u03b22, and -\u03b23 secreted by a human glioblastoma cell line: identification of small and different forms of large latent complexes.","volume":"267","author":"Olofsson","year":"1992","journal-title":"J. Biol. 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Cell Biol."},{"key":"2026030700201118100_B8","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1146\/annurev.cb.06.110190.003121","article-title":"The transforming growth factor-\u03b2 family.","volume":"6","author":"Massague","year":"1990","journal-title":"Annu. Rev. Cell Biol."},{"key":"2026030700201118100_B9","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/0962-8924(93)90195-7","article-title":"Receptors for the TGF-\u03b2 superfamily: multiple polypeptides and serine\/threonine kinases.","volume":"3","author":"Lin","year":"1993","journal-title":"Trends Cell Biol."},{"key":"2026030700201118100_B10","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/S0955-0674(96)80058-5","article-title":"Signaling via hetero-oligomeric complexes of type I and type II serine\/threonine kinase receptors.","volume":"8","author":"ten Dijke","year":"1996","journal-title":"Curr. Opin. 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