{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T12:51:52Z","timestamp":1773838312699,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1990,2,1]],"date-time":"1990-02-01T00:00:00Z","timestamp":633830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1990,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The number of neutrophils in the lungs of cigarette smokers is increased. This could be a consequence of the chemotactic mediator synthesis by alveolar macrophages (AM). In order to evaluate the possible role of leukotriene B4 (LTB4) in this condition, we studied the formation of LTB4 by nonsmokers' AM exposed in vitro and in vivo to cigarette smoke and by smokers' AM. In the absence of stimulus or upon stimulation, nonsmokers' AM exposed in vitro to tobacco smoke formed less LTB4 than did nonexposed AM, e.g., the cells incubated with arachidonic acid and ionophore produced, respectively, 241 \u00b1 132 and 425 \u00b1 106 pmol LTB4\/106 cells (mean \u00b1 SEM) (P &amp;lt; 0.01). In other experiments, smokers' AM were incubated in absence of stimulus and produced less LTB4 than did nonsmokers' AM; during a 3-h incubation, smokers' and nonsmokers' adherent AM released, respectively, 3 \u00b1 2 and 40 \u00b1 28 pmol LTB4\/106 cells (P &amp;lt; 0.05). Similarly stimulated smokers' AM produced less LTB4 than did nonsmokers' AM, e.g., the cells incubated with arachidonic acid and ionophore formed, respectively, 225 \u00b1 41 and 573 \u00b1 150 pmol LTB4\/106 cells (P &amp;lt; 0.05). In a group including mild smokers and nonsmokers, in vivo exposure to the smoke of 4 cigarettes produced a decrease in the release of LTB4 by AM, e.g., in the presence of arachidonic acid and ionophore, nonexposed and exposed AM produced, respectively, 198 \u00b1 38 and 143 \u00b1 38 pmol LTB4\/106 cells (P &amp;lt; 0.05). In all those conditions, AM exposed to cigarette smoke exhibited an inhibition of 5-hydroxy-eicosatetraenoic acid. These results show that acute in vitro or in vivo exposures to tobacco smoke and chronic smoking inhibit the spontaneous and stimulated AM production of arachidonic acid metabolites. Consequently, it is very unlikely that LTB4 could account for the neutrophil recruitment into the lungs of smokers.<\/jats:p>","DOI":"10.1165\/ajrcmb\/2.2.155","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T23:11:20Z","timestamp":1365030680000},"page":"155-161","source":"Crossref","is-referenced-by-count":18,"title":["Inhibition of Human Alveolar Macrophage Production of Leukotriene B4 by Acute\n                    <i>In Vitro<\/i>\n                    and\n                    <i>In Vivo<\/i>\n                    Exposure to Tobacco Smoke"],"prefix":"10.1093","volume":"2","author":[{"given":"Johanne","family":"Tardif","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Recherche, Centre de Pneumologie, H\u00f4pital, Ste-Foy, and Unit\u00e9 de Recherche, Inflammation et Immunologie-Rhumatologie, Centre Hospitalier de l'Universit\u00e9 Laval, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Borgeat","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche, Centre de Pneumologie, H\u00f4pital, Ste-Foy, and Unit\u00e9 de Recherche, Inflammation et Immunologie-Rhumatologie, Centre Hospitalier de l'Universit\u00e9 Laval, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Laviolette","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche, Centre de Pneumologie, H\u00f4pital, Ste-Foy, and Unit\u00e9 de Recherche, Inflammation et Immunologie-Rhumatologie, Centre Hospitalier de l'Universit\u00e9 Laval, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1990,2,1]]},"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/2\/2\/155\/67278906\/ajrcmb_2_2_155.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/2\/2\/155\/67278906\/ajrcmb_2_2_155.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T09:26:55Z","timestamp":1773826015000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/2\/2\/155\/8511938"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1990,2,1]]},"published-print":{"date-parts":[[1990,2,1]]}},"URL":"https:\/\/doi.org\/10.1165\/ajrcmb\/2.2.155","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1990,2]]},"published":{"date-parts":[[1990,2,1]]}}}