{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T12:50:07Z","timestamp":1773838207980,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1990,4,1]],"date-time":"1990-04-01T00:00:00Z","timestamp":638928000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1990,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Epithelial shedding is a characteristic feature of asthmatic airways and has been attributed to eosinophil products. We have examined the interaction of purified intraperitoneal guinea pig eosinophils with or without platelet-activating factor (PAF, 10\u22127 M) or lyso-PAF (10\u22127 M) with guinea pig tracheal epithelium in vitro. At 0, 4, 14, and 24 h, the percentage of ciliation of the tracheal circumference (CTC) was measured by light microscopy and the ciliary beat frequency (CBF) by photometry. PAF-activated eosinophils (50 \u00d7 106 cells\/ml) disrupted the epithelium, mean CBF and CTC being reduced by 77.8 \u00b1 5.8% (mean \u00b1 SEM; P &amp;lt; 0.001 versus control) and 94.2 \u00b1 1.4% (P &amp;lt; 0.001) over 24 h, respectively. PAF (10\u22127 M) alone had no significant effect. Lyso-PAF with eosinophils (50 \u00d7 106 cells\/ml) also reduced mean CBF and CTC but to a lesser extent. Eosinophils alone also led to a reduction of 36.2 + 11.4% in mean CBF and 53.0 + 15.5% in CTC, but these changes were not significant. The PAF antagonist, WEB 2086 (106 M), significantly inhibited the mean CBF and CTC reduction due to PAF-activated eosinophils by 61.5 \u00b1 17.2% (P &amp;lt; 0.01) and 20.8 \u00b1 6.5% (P &amp;lt; 0.05), respectively. In addition, catalase (1,125 U\/ml) partially inhibited the mean CBF and CTC reduction induced by PAF-activated eosinophils. Intraperitoneal neutrophils (PMN) (50 \u00d7 106 cells\/ml) also disrupted epithelium but to a lesser extent (24-h reduction: 34.2 \u00b1 12.7% for mean CBF and 60.2 \u00b1 13.2% for CTC, respectively). Stimulation with PAF (10\u22127 M) had no further effect. Marked exfoliation of the epithelial layer was observed after 14 h of incubation with activated eosinophils. We concluded the PAF-activated eosinophils are capable of grossly disrupting ciliated epithelium and may contribute to epithelial damage observed in asthma.<\/jats:p>","DOI":"10.1165\/ajrcmb\/2.4.341","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T23:08:08Z","timestamp":1365030488000},"page":"341-353","source":"Crossref","is-referenced-by-count":65,"title":["The Effects of Activated Eosinophils and Neutrophils on Guinea Pig Airway Epithelium\n                    <i>In Vitro<\/i>"],"prefix":"10.1093","volume":"2","author":[{"given":"Tatsuo","family":"Yukawa","sequence":"first","affiliation":[{"name":"Department of Thoracic Medicine, National Heart and Lung Institute, Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert C.","family":"Read","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, National Heart and Lung Institute, Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus","family":"Kroegel","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, National Heart and Lung Institute, Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Rutman","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, National Heart and Lung Institute, Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. Fan","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, National Heart and Lung Institute, Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, National Heart and Lung Institute, Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Cole","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, National Heart and Lung Institute, Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Barnes","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine, National Heart and Lung Institute, Brompton Hospital, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1990,4,1]]},"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/2\/4\/341\/67278544\/ajrcmb_2_4_341.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/2\/4\/341\/67278544\/ajrcmb_2_4_341.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T09:25:51Z","timestamp":1773825951000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/2\/4\/341\/8511922"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[1990,4,1]]},"published-print":{"date-parts":[[1990,4,1]]}},"URL":"https:\/\/doi.org\/10.1165\/ajrcmb\/2.4.341","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1990,4]]},"published":{"date-parts":[[1990,4,1]]}}}