{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:58:29Z","timestamp":1787018309118,"version":"build-2736575974"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[1991,11,1]],"date-time":"1991-11-01T00:00:00Z","timestamp":688953600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We examined the importance of the cytokine tumor necrosis factor-\u03b1 (TNF-\u03b1) in a mouse model of hypersensitivity pneumonitis (HP). Mice of the C57BL\/6 strain were instilled intranasally 3 days\/wk for 3 wk with 150 \u00b5g of the actinomycete Faenia rectivirgula (Micropolyspora faeni) to induce HP as a model of farmer's lung. This experimental model was associated with a progressive inflammation in the lungs of challenged mice, seen histologically as cellular infiltrates of large quantities of macrophages and lymphocytes and some neutrophils. The disease in challenged mice treated with a control rabbit serum was also associated with a substantial release of tumor TNF-\u03b1 (up to 80 U\/ml of TNF-\u03b1 in the bronchoalveolar lavage [BAL] at 3 wk after beginning of treatment) and interleukin-1, which peaked at 1 wk (\u223c300 U\/ml) and diminished thereafter. A very large increase in BAL cell number (11-fold increase versus saline controls) and an enhanced release potential for TNF-\u03b1 by alveolar macrophages was also seen. Lung fibrosis was also evident in challenged animals, as demonstrated by a 2-fold increase in hydroxyproline levels. Infusion of challenged mice with a rabbit polyclonal antibody against TNF-\u03b1 (2 mg\/wk) completely abrogated the disease, as mice so treated had normal lung histology. Anti-TNF-\u03b1 blocked cellular recruitment in the lungs (only a 2-fold increase at week 3); it also completely abolished TNF-\u03b1 secretion in the BAL and drastically reduced interleukin-1 levels in this fluid. Anti-TNF-\u03b1 also abolished lung index increases and lung fibrosis, with both parameters similar to that of saline-instilled mice. These findings indicate a crucial role for TNF-\u03b1 in the development of experimental HP.<\/jats:p>","DOI":"10.1165\/ajrcmb\/5.5.477","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T23:09:56Z","timestamp":1365030596000},"page":"477-483","source":"Crossref","is-referenced-by-count":65,"title":["Tumor Necrosis Factor Plays an Essential Role in Determining Hypersensitivity Pneumonitis in a Mouse Model"],"prefix":"10.1093","volume":"5","author":[{"given":"Michel","family":"Denis","sequence":"first","affiliation":[{"name":"Unit\u00e9 de recherche, Centre de Pneumologie et D\u00e9partement de Pathologie de l'H\u00f4pital et l'Universit\u00e9 Laval, H\u00f4pital Laval, Sainte-Foy, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvon","family":"Cormier","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de recherche, Centre de Pneumologie et D\u00e9partement de Pathologie de l'H\u00f4pital et l'Universit\u00e9 Laval, H\u00f4pital Laval, Sainte-Foy, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcien","family":"Fournier","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de recherche, Centre de Pneumologie et D\u00e9partement de Pathologie de l'H\u00f4pital et l'Universit\u00e9 Laval, H\u00f4pital Laval, Sainte-Foy, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanne","family":"Tardif","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de recherche, Centre de Pneumologie et D\u00e9partement de Pathologie de l'H\u00f4pital et l'Universit\u00e9 Laval, H\u00f4pital Laval, Sainte-Foy, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Laviolette","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de recherche, Centre de Pneumologie et D\u00e9partement de Pathologie de l'H\u00f4pital et l'Universit\u00e9 Laval, H\u00f4pital Laval, Sainte-Foy, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1991,11,1]]},"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/5\/5\/477\/67277340\/ajrcmb_5_5_477.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/5\/5\/477\/67277340\/ajrcmb_5_5_477.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T09:52:31Z","timestamp":1773827551000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/5\/5\/477\/8511687"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1991,11,1]]},"published-print":{"date-parts":[[1991,11,1]]}},"URL":"https:\/\/doi.org\/10.1165\/ajrcmb\/5.5.477","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1991,11]]},"published":{"date-parts":[[1991,11,1]]}}}