{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T14:57:46Z","timestamp":1773845866003,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[1992,1,1]],"date-time":"1992-01-01T00:00:00Z","timestamp":694224000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1992,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Platelet-activating factor (PAF) is a potent inflammatory mediator that can cause airway obstruction and hyperresponsiveness; these processes are also associated with pulmonary eosinophilia, suggesting a link between these two events. Thus, PAF's interaction with eosinophils may provide a mechanism for airway damage. However, direct in vitro activation of eosinophils by PAF requires concentrations that are likely higher than those achieved in vivo. As a result, we investigated whether lower, more physiologic concentrations of PAF could prime eosinophils for subsequent activation to another receptor-stimulated factor, in this case formylmethionylleucylphenylalanine (FMLP). To test this hypothesis, eosinophils were preincubated (1 and 15 min) with low concentrations of PAF (1 \u00d7 10\u22128 and 1 \u00d7 10\u221210 M); this exposure to PAF resulted in enhanced generation of superoxide anion to FMLP stimulation. Moreover, similar concentrations of PAF decreased eosinophil density and increased expression of cell surface CR3 receptors. Finally, low, nonactivating concentrations of PAF (1 \u00d7 10\u221210 to 1 \u00d7 10\u22128 M) caused transient increases in eosinophil cytosolic free Ca2+ concentrations. Collectively, these responses are consistent with the hypothesis that short-term exposure to low concentrations of PAF primes eosinophils to cause an enhanced inflammatory response upon subsequent activation to another receptor agonist. The consequences of this PAF-associated phenomenon can produce an enhanced inflammatory response and airway injury.<\/jats:p>","DOI":"10.1165\/ajrcmb\/6.1.100","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T22:46:19Z","timestamp":1365029179000},"page":"100-106","source":"Crossref","is-referenced-by-count":12,"title":["Platelet-activating Factor Primes Human Eosinophil Generation of Superoxide"],"prefix":"10.1093","volume":"6","author":[{"given":"Edward M.","family":"Zoratti","sequence":"first","affiliation":[{"name":"Department of Medicine, Section of Allergy and Immunology, University of Wisconsin Medical School, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie B.","family":"Sedgwick","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Allergy and Immunology, University of Wisconsin Medical School, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rose F.","family":"Vrtis","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Allergy and Immunology, University of Wisconsin Medical School, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"Ellen Bates","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Allergy and Immunology, University of Wisconsin Medical School, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristen","family":"Geiger","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Allergy and Immunology, University of Wisconsin Medical School, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William W.","family":"Busse","sequence":"additional","affiliation":[{"name":"Department of Medicine, Section of Allergy and Immunology, University of Wisconsin Medical School, Madison, Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1992,1,1]]},"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/6\/1\/100\/67277120\/ajrcmb_6_1_100.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/6\/1\/100\/67277120\/ajrcmb_6_1_100.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T09:53:29Z","timestamp":1773827609000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/6\/1\/100\/8510434"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[1992,1,1]]},"published-print":{"date-parts":[[1992,1,1]]}},"URL":"https:\/\/doi.org\/10.1165\/ajrcmb\/6.1.100","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1992,1]]},"published":{"date-parts":[[1992,1,1]]}}}