{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T07:56:05Z","timestamp":1789977365282,"version":"4.0.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[1993,5,1]],"date-time":"1993-05-01T00:00:00Z","timestamp":736214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1993,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Human alveolar macrophages (AM) can produce potent reactive oxygen intermediates (ROI) and arachidonic acid metabolites (eicosanoids), which have important roles in host defense and the pathogenesis of some diseases of the lung. Bacterial lipopolysaccharide (LPS) is believed to cause profound lung injury and can prime mouse peritoneal macrophages for the enhanced secretion of ROI and eicosanoids. There- fore, we investigated the effect of LPS pretreatment on the ability of AM to release superoxide anions (O\u2212 \u00a02) and leukotriene B4 (LTB4). LPS can prime AM for the enhanced secretion of O\u2212 \u00a02 and LTB4, regardless of whether they are derived from nonsmokers or smokers. Moreover, judging from the time-response characteristics, this priming for LTB4 release could be inhibited in the later stages of pretreatment, when the O\u2212 \u00a02-releasing capacity was enhanced. The priming inhibition was prevented, at least in part, by cycloheximide, but not by SOD and\/or catalase. In addition, cycloheximide also inhibited the priming for O\u2212 \u00a02 release. Hence, protein synthesis might be necessary for the priming for O\u2212 \u00a02 release and for inhibiting the priming for LTB4 release. This phenomenon of self-limiting the priming response with LPS seems to be very important when we consider the high oxygen tension in the lungs and the many bacterial substances inspired into alveoli.<\/jats:p>","DOI":"10.1165\/ajrcmb\/8.5.500","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T22:30:10Z","timestamp":1365028210000},"page":"500-508","source":"Crossref","is-referenced-by-count":26,"title":["Lipopolysaccharide Primes Human Alveolar Macrophages for Enhanced Release of Superoxide Anion and Leukotriene B4: Self-Limitations of the Priming Response with Protein Synthesis"],"prefix":"10.1093","volume":"8","author":[{"given":"Katsuhiro","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Infection and Inflammation, First Clinic of Medicine, Chest Disease Research Institute, and First Department of Internal Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Infection and Inflammation, First Clinic of Medicine, Chest Disease Research Institute, and First Department of Internal Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuo","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Infection and Inflammation, First Clinic of Medicine, Chest Disease Research Institute, and First Department of Internal Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takako","family":"Murayama","sequence":"additional","affiliation":[{"name":"Department of Infection and Inflammation, First Clinic of Medicine, Chest Disease Research Institute, and First Department of Internal Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryouichi","family":"Amitani","sequence":"additional","affiliation":[{"name":"Department of Infection and Inflammation, First Clinic of Medicine, Chest Disease Research Institute, and First Department of Internal Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kokichi","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Infection and Inflammation, First Clinic of Medicine, Chest Disease Research Institute, and First Department of Internal Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiyuki","family":"Kuze","sequence":"additional","affiliation":[{"name":"Department of Infection and Inflammation, First Clinic of Medicine, Chest Disease Research Institute, and First Department of Internal Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1993,5,1]]},"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/8\/5\/500\/67276479\/ajrcmb_8_5_500.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/8\/5\/500\/67276479\/ajrcmb_8_5_500.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T09:57:56Z","timestamp":1773827876000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/8\/5\/500\/8511510"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1993,5,1]]},"published-print":{"date-parts":[[1993,5,1]]}},"URL":"https:\/\/doi.org\/10.1165\/ajrcmb\/8.5.500","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1993,5]]},"published":{"date-parts":[[1993,5,1]]}}}