{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T06:56:45Z","timestamp":1773730605600,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[1995,3,1]],"date-time":"1995-03-01T00:00:00Z","timestamp":794016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Airway inflammation in acute and chronic bronchitis includes a prominent neutrophil influx. Using a rat model of sulfur dioxide (SO2)-induced bronchitis, we investigated the role of the polymorphonuclear leukocyte (PMN) chemokines macrophage inflammatory protein-2 (MIP-2) and KC. Adult female rats were exposed to 230 ppm SO2 for 5 h\/day for periods of 1 day to 5 wk. Immunohistochemical identification of rat PMNs in trachea cryostat sections allowed quantitation of a marked neutrophil influx into airways of bronchitic rats (PMNs\/trachea ring = 55 +\/- 26.2 [1 day SO2] versus 3.6 +\/- 2.7 [air]; n = 5, P &amp;lt; or = 0.05). Northern analysis of trachea homogenates demonstrated induction of KC and MIP-2 mRNA expression after 1 day of SO2 and persistence of increased expression after longer exposure periods examined. Pretreatment of rats with dexamethasone (0.5 mg\/kg) prior to a 1-day acute SO2 exposure prevented induction of chemokine mRNA and abrogated neutrophil influx completely (PMNs\/trachea ring = 6.6 +\/- 8.8 versus air controls; n = 5, P = 0.96). To determine if chemokine inhibition by dexamethasone could be further studied in vitro, the rat alveolar macrophage cell line NR8383 was treated with dexamethasone (10(-7) M) before stimulation with lipopolysaccharide (10 micrograms\/ml). Pretreatment with dexamethasone substantially decreased induction of both MIP-2 and KC mRNA in response to lipopolysaccharide, indicating the potential utility of in vitro systems to identify additional anti-inflammatory agents. These studies support the hypothesis that the chemokines MIP-2 and KC mediate airway neutrophil influx in both acute and chronic SO2-induced bronchitis in the rat.<\/jats:p>","DOI":"10.1165\/ajrcmb.12.3.7873201","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T22:28:47Z","timestamp":1365028127000},"page":"345-350","source":"Crossref","is-referenced-by-count":31,"title":["Airway Neutrophilia and Chemokine mRNA Expression in Sulfur Dioxide-Induced Bronchitis"],"prefix":"10.1093","volume":"12","author":[{"given":"A","family":"Farone","sequence":"first","affiliation":[{"name":"Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Paulauskis","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Kobzik","sequence":"additional","affiliation":[{"name":"Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1995,3,1]]},"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/12\/3\/345\/67373819\/ajrcmb_12_3_345.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/12\/3\/345\/67373819\/ajrcmb_12_3_345.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T05:03:19Z","timestamp":1773723799000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/12\/3\/345\/8525399"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1995,3,1]]},"published-print":{"date-parts":[[1995,3,1]]}},"URL":"https:\/\/doi.org\/10.1165\/ajrcmb.12.3.7873201","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1995,3]]},"published":{"date-parts":[[1995,3,1]]}}}