{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T22:50:36Z","timestamp":1774565436491,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1996,2,1]],"date-time":"1996-02-01T00:00:00Z","timestamp":823132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1996,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Interleukin-10 (IL-10) inhibits T-lymphocyte proliferation and production of cytokines. We have examined expression of IL-10 messenger RNA (mRNA) in atopic asthma and in allergen and tuberculin skin responses by in situ hybridization. The proportion of bronchoalveolar lavage (BAL) cells positive for IL-10 mRNA was increased in a group of 10 symptomatic asthmatics when compared with control subjects (17.5% versus 5.2% BAL cells positive; P &amp;lt; 0.001). In a separate group of six mild atopic asthmatics, there was an increased proportion of BAL cells positive for IL-10 mRNA 24 h after allergen inhalation challenge compared with diluent challenge BAL from the same subjects (24% versus 10%; P &amp;lt; 0.005). By simultaneous in situ hybridization and immunocytochemistry, IL-10 mRNA was localized to both CD3+ T cells and CD68+ alveolar macrophages in BAL, with a significantly more prominent T-cell signal in the symptomatic asthmatics compared with control subjects and after allergen challenge compared with diluent challenge of the mild asthmatic subjects. It has been suggested that IL-10 production is a late event after T-cell activation. To examine kinetics and specificity of IL-10 mRNA expression, skin biopsies were obtained from atopic, tuberculin-sensitive subjects at 1, 6, and 48 h after cutaneous injection of allergen or tuberculin. With both stimuli, there was an increase in IL-10 mRNA-positive cells at 6 h when compared with control sites injected with appropriate diluent which were biopsied 24 h after injection (P &amp;lt; 0.01 for allergen and P &amp;lt; 0.02 for tuberculin). These findings are compatible with the hypothesis that IL-10 mRNA is expressed in both macrophages and T lymphocytes in the airway in asthma and that IL-10 mRNA expression is induced from T lymphocytes in response to allergen. This response may also occur in other types of cell-mediated inflammation.<\/jats:p>","DOI":"10.1165\/ajrcmb.14.2.8630259","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T22:27:43Z","timestamp":1365028063000},"page":"113-117","source":"Crossref","is-referenced-by-count":109,"title":["Increased Interleukin-10 Messenger RNA Expression in Atopic Allergy and Asthma"],"prefix":"10.1093","volume":"14","author":[{"given":"D S","family":"Robinson","sequence":"first","affiliation":[{"name":"National Heart and Lung Institute, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Tsicopoulos","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q","family":"Meng","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Durham","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A B","family":"Kay","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q","family":"Hamid","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1996,2,1]]},"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/14\/2\/113\/67384874\/ajrcmb_14_2_113.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/14\/2\/113\/67384874\/ajrcmb_14_2_113.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T02:12:19Z","timestamp":1773799939000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/14\/2\/113\/8526749"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1996,2,1]]},"published-print":{"date-parts":[[1996,2,1]]}},"URL":"https:\/\/doi.org\/10.1165\/ajrcmb.14.2.8630259","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1996,2]]},"published":{"date-parts":[[1996,2,1]]}}}