{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T19:20:39Z","timestamp":1778181639349,"version":"3.51.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1996,8,1]],"date-time":"1996-08-01T00:00:00Z","timestamp":838857600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1996,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Alkylating agents may cause DNA damage in different human cells and tissues, including lungs. For instance, tobacco-specific N-nitrosamines are known to produce methyl-DNA adducts, such as N7-methyldeoxyguanosine, and to induce lung tumors. We applied a combined high-performance liquid chromatography (HPLC)\/32P-postlabeling technique for measurement of N7-methyldeoxyguanosine in human pulmonary alveolar cells (HPAC). Thirty patients (13 males, 17 females; mean age 51 +\/- 17 yr) undergoing bronchoalveolar lavage for diagnosis of nonmalignant lung diseases were studied. DNA was extracted from HPAC, digested to 2\u2032-deoxyribonucleotide 3\u2032-monophosphates and HPLC separated to obtain deoxyguanosine (dGp) and N7-methyldeoxyguanosine (N7-MedGp) monophosphates. Fractions corresponding to normal (1:10,000) and N7-methylated dGp were subsequently 32P-postlabeled by T4 polynucleotide kinase with high specific activity 32P-ATP, resolved by two-dimensional thin-layer chromatography (TLC) and autoradiographed after 3 to 18 h exposure. Spots corresponding to dGp and N7-MedGp were scraped off the plates and quantitated by liquid scintillation counting to calculate direct molar ratios. Recovered HPAC (14.4 +\/- 10.0 x 10(6)) were predominantly macrophages (73.8 +\/- 16.4%) and lymphocytes (9.8 +\/- 11.6%). N7-MedGp was detected in 11 patients, the level ranging from 0.10 to 48.03 fmol\/micrograms DNA which corresponded to 0.31-79.00 x 10(-6) N7-MedGp\/dGp ratios. Detection of N7-MedGp in HPAC was associated with the smoking habit of patients: N7-MedGp was present in 7 of 10 smokers, 2 of 10 ex-smokers, and 2 of 10 nonsmokers (P &amp;lt; 0.05). These results show that HPAC may be used for molecular dosimetry of DNA damage by alkylating agents, including tobacco-specific N-nitrosamines, in cigarette smokers and thus used for cancer risk assessment.<\/jats:p>","DOI":"10.1165\/ajrcmb.15.2.8703477","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T22:28:42Z","timestamp":1365028122000},"page":"216-223","source":"Crossref","is-referenced-by-count":21,"title":["Detection of N7-Methyldeoxyguanosine Adducts in Human Pulmonary Alveolar Cells"],"prefix":"10.1093","volume":"15","author":[{"given":"S","family":"Petruzzelli","sequence":"first","affiliation":[{"name":"U.O. Fisiopatologia Respiratoria, Clinica Medica 2a, Universit\u00e0 di Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L M","family":"Tavanti","sequence":"additional","affiliation":[{"name":"U.O. Fisiopatologia Respiratoria, Clinica Medica 2a, Universit\u00e0 di Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Celi","sequence":"additional","affiliation":[{"name":"U.O. Fisiopatologia Respiratoria, Clinica Medica 2a, Universit\u00e0 di Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Giuntini","sequence":"additional","affiliation":[{"name":"U.O. Fisiopatologia Respiratoria, Clinica Medica 2a, Universit\u00e0 di Pisa, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1996,8,1]]},"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/15\/2\/216\/67385137\/ajrcmb_15_2_216.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/15\/2\/216\/67385137\/ajrcmb_15_2_216.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T02:20:28Z","timestamp":1773800428000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/15\/2\/216\/8526834"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1996,8,1]]},"published-print":{"date-parts":[[1996,8,1]]}},"URL":"https:\/\/doi.org\/10.1165\/ajrcmb.15.2.8703477","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1996,8]]},"published":{"date-parts":[[1996,8,1]]}}}