{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:46:49Z","timestamp":1772930809691,"version":"3.50.1"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[1997,12,1]],"date-time":"1997-12-01T00:00:00Z","timestamp":880934400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Eotaxin is an eosinophil-specific chemokine associated with the recruitment of eosinophils to the site of allergic inflammation. The aims of this study were to determine the expression of eotaxin in nasal biopsies from allergic and nonallergic individuals with chronic severe sinusitis, and to examine whether the expression of this chemokine is upregulated following allergen challenge in the nasal mucosa of patients with allergic rhinitis. We also undertook to phenotype of inflammatory cells within the submucosa expressing eotaxin mRNA. Nasal turbinate tissue from 16 individuals with allergic or nonallergic chronic sinusitis and 10 normal controls were examined for the presence of eotaxin mRNA and immunoreactivity by in situ hybridization and immunocytochemistry. The numbers of cells expressing eotaxin mRNA were also determined after either allergen or diluent challenge in atopic subjects with a history of allergic rhinitis. There was a constitutive expression of eotaxin-immunoreactivity and the presence of eotaxin mRNA-positive cells in nasal biopsies from normal individuals. Compared with normal controls, the numbers of cells expressing eotaxin mRNA and protein were significantly increased in both allergic and nonallergic sinusitis (P &amp;lt; 0.001). Eotaxin mRNA was expressed by nasal epithelial cells and primarily colocalized to CD68-positive macrophages within the subepithelium. In subjects with allergic rhinitis, allergen challenge markedly increased the numbers of cells expressing eotaxin mRNA and immunoreactivity in the epithelial and subepithelial cell layers (P &amp;lt; 0.05). This could be largely attributed to a local increase in eotaxin production within the nasal tissues. The results of this study demonstrate the constitutive expression of eotaxin and show that the numbers of cells expressing eotaxin mRNA are increased within the epithelial and subepithelial layers of the nasal mucosa in individuals with chronic sinusitis. Furthermore, allergen challenge of the nasal mucosa in atopic subjects results in a local upregulation of eotaxin expression. These data suggest a potential role for this chemokine in the pathogenesis of allergic and nonallergic eosinophilic inflammation characterizing chronic sinusitis and allergic rhinitis.<\/jats:p>","DOI":"10.1165\/ajrcmb.17.6.2865","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T22:24:42Z","timestamp":1365027882000},"page":"683-690","source":"Crossref","is-referenced-by-count":156,"title":["Eotaxin mRNA and Protein Expression in Chronic Sinusitis and Allergen-induced Nasal Responses in Seasonal Allergic Rhinitis"],"prefix":"10.1093","volume":"17","author":[{"given":"E. M.","family":"Minshall","sequence":"first","affiliation":[{"name":"Meakins-Christie Laboratories, McGill University; Notre Dame Hospital, Montreal, Quebec, Canada; National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado; Infectious Diseases Unit, Massachusetts General Hospital; Harvard Medical School, Boston, Massachusetts; and Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Cameron","sequence":"additional","affiliation":[{"name":"Meakins-Christie Laboratories, McGill University; Notre Dame Hospital, Montreal, Quebec, Canada; National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado; Infectious Diseases Unit, Massachusetts General Hospital; Harvard Medical School, Boston, Massachusetts; and Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Lavigne","sequence":"additional","affiliation":[{"name":"Meakins-Christie Laboratories, McGill University; Notre Dame Hospital, Montreal, Quebec, Canada; National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado; Infectious Diseases Unit, Massachusetts General Hospital; Harvard Medical School, Boston, Massachusetts; and Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Y. M.","family":"Leung","sequence":"additional","affiliation":[{"name":"Meakins-Christie Laboratories, McGill University; Notre Dame Hospital, Montreal, Quebec, Canada; National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado; Infectious Diseases Unit, Massachusetts General Hospital; Harvard Medical School, Boston, Massachusetts; and Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Hamilos","sequence":"additional","affiliation":[{"name":"Meakins-Christie Laboratories, McGill University; Notre Dame Hospital, Montreal, Quebec, Canada; National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado; Infectious Diseases Unit, Massachusetts General Hospital; Harvard Medical School, Boston, Massachusetts; and Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. A.","family":"Garcia-Zepada","sequence":"additional","affiliation":[{"name":"Meakins-Christie Laboratories, McGill University; Notre Dame Hospital, Montreal, Quebec, Canada; National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado; Infectious Diseases Unit, Massachusetts General Hospital; Harvard Medical School, Boston, Massachusetts; and Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Rothenberg","sequence":"additional","affiliation":[{"name":"Meakins-Christie Laboratories, McGill University; Notre Dame Hospital, Montreal, Quebec, Canada; National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado; Infectious Diseases Unit, Massachusetts General Hospital; Harvard Medical School, Boston, Massachusetts; and Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. D.","family":"Luster","sequence":"additional","affiliation":[{"name":"Meakins-Christie Laboratories, McGill University; Notre Dame Hospital, Montreal, Quebec, Canada; National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado; Infectious Diseases Unit, Massachusetts General Hospital; Harvard Medical School, Boston, Massachusetts; and Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"Hamid","sequence":"additional","affiliation":[{"name":"Meakins-Christie Laboratories, McGill University; Notre Dame Hospital, Montreal, Quebec, Canada; National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado; Infectious Diseases Unit, Massachusetts General Hospital; Harvard Medical School, Boston, Massachusetts; and Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1997,12,1]]},"reference":[{"key":"2026030700202778500_B1","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1164\/ajrccm\/141.3.689","article-title":"The neutrophil and chronic allergic inflammation: immunochemical localization of neutrophil elastase.","volume":"141","author":"Fujisawa","year":"1990","journal-title":"Am. 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Immunol."},{"key":"2026030700202778500_B4","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1164\/ajrccm\/146.1.170","article-title":"Immunohistology of the nasal mucosa following allergen-induced rhinitis: identification of activated T cells, eosinophils and neutrophils.","volume":"146","author":"Varney","year":"1992","journal-title":"Am. Rev. Respir. Dis."},{"key":"2026030700202778500_B5","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/0091-6749(94)90140-6","article-title":"Biochemical properties of eosinophils and their preferential accumulation mechanism in nasal allergy.","volume":"94","author":"Terada","year":"1994","journal-title":"J. Allergy Clin. 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Invest."},{"key":"2026030700202778500_B14","doi-asserted-by":"crossref","first-page":"2390","DOI":"10.4049\/jimmunol.148.8.2390","article-title":"Cytokine messenger mRNA expression for IL-3, IL-4, IL-5 and granulocyte\/macrophage colony-stimulating factor in the nasal mucosa after local allergen provocation: relationship to tissue eosinophilia.","volume":"148","author":"Durham","year":"1992","journal-title":"J. Immunol."},{"key":"2026030700202778500_B15","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1172\/JCI115166","article-title":"Expression of mRNA for interleukin-5 in mucosal bronchial biopsies from asthma.","volume":"87","author":"Hamid","year":"1991","journal-title":"J. Clin. 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