{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:46:13Z","timestamp":1772930773502,"version":"3.50.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1998,2,1]],"date-time":"1998-02-01T00:00:00Z","timestamp":886291200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We investigated the effects of in vivo intraperitoneal treatment with the rat monoclonal antibody (mAb), YN1.7.4 (YN1) against intercellular adhesion molecule-1 (ICAM-1) on the ovalbumin (OA)-inhalation-induced infiltration of leukocytes into the airways of OA-sensitized mice. YN1 (100 to 400 \u03bcg) given over a period of 72 h dose-dependently reduced the influx of lymphocytes and eosinophils into the bronchial lumen by &amp;gt; 60% and \u2265 70%, respectively, when compared with saline or purified rat IgG-treated controls. Alveolar macrophages (AM) in the bronchoalveolar lavage fluid (BALF) were also decreased by &amp;gt; 50%. Lung tissue inflammation as determined by histopathologic examination was reduced. The number of neutrophils in the blood of OA-sensitized mice 3 days after challenge was significantly increased by treatment with YN1. However, at 24 h and 72 h after OA-challenge, the numbers of eosinophils and mononuclear cells in the bone marrow were reduced by YN1 treatment. Additionally, at 72 h after OA-challenge, the numbers of bone-marrow neutrophils were depressed. BALF levels of interleukin-5 (IL-5) and of IgA were lower for YN1-treated mice than for controls. With increasing doses of YN1, the levels of anti-ICAM-1 mAb in the plasma were proportionally increased. To correlate these results with YN1 treatment, blood and BALF T cells and BALF eosinophils were examined with flow cytometry. Blood T cells from YN1-treated mice were unable to bind phycoerythrin (PE)-labeled anti-ICAM-1 mAb ex vivo. These results implied that ICAM-1 on these cells was bound (occupied) by YN1 administered in vivo. Dose-related decreases were observed in the percentage and mean channel fluorescence (MCF) values of ICAM-1+ BALF T cells and eosinophils. The percentages of CD11a+ or CD49d+ eosinophils were also suppressed. Our data suggest that ICAM-1 is an important molecule involved in the recruitment of leukocytes into the airways of sensitized mice after pulmonary challenge.<\/jats:p>","DOI":"10.1165\/ajrcmb.18.2.2565m","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T22:13:30Z","timestamp":1365027210000},"page":"158-167","source":"Crossref","is-referenced-by-count":27,"title":["Involvement of Intercellular Adhesion Molecule-1 in the Antigen-induced Infiltration of Eosinophils and Lymphocytes into the Airways in a Murine Model of Pulmonary Inflammation"],"prefix":"10.1093","volume":"18","author":[{"given":"Jia En","family":"Chin","sequence":"first","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greg E.","family":"Winterrowd","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl A.","family":"Hatfield","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Brashler","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Griffin","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven L.","family":"Vonderfecht","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen P.","family":"Kolbasa","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen F.","family":"Fidler","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathy L.","family":"Shull","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond F.","family":"Krzesicki","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathleen A.","family":"Ready","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin J.","family":"Dunn","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurel M.","family":"Sly","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nigel D.","family":"Staite","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan M.","family":"Richards","sequence":"additional","affiliation":[{"name":"Cell Biology and Inflammation Research, and Drug Development Toxicology, Pharmacia and Upjohn Inc., Kalamazoo, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1998,2,1]]},"reference":[{"key":"2026030700194005700_B1","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1164\/ajrccm\/142.6_Pt_1.1407","article-title":"Identification of activated T lymphocytes and eosinophils in bronchial biopsies in stable atopic asthma.","volume":"142","author":"Azzawi","year":"1990","journal-title":"Am. 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