{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:46:26Z","timestamp":1772930786868,"version":"3.50.1"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1998,2,1]],"date-time":"1998-02-01T00:00:00Z","timestamp":886291200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Adhesion molecule expression by pulmonary endothelial cells is considered to play an important role in the recruitment of circulating leukocytes to sites of inflammation in the lung. We have used P-selectin- and intercellular adhesion molecule type 1 (ICAM-1)-deficient mice to determine whether these adhesion molecules are important to pulmonary eosinophil recruitment after allergen challenge. There was a significant inhibition of lung tissue eosinophil recruitment in ICAM-1-deficient mice (\u223c 84% inhibition compared to wild-type mice) and P-selectin-deficient mice (\u223c 67% inhibition compared to wild-type mice) 3 h after allergen challenge. The number of bronchoalveolar lavage (BAL) eosinophils in P-selectin-deficient and ICAM-1-deficient mice was also significantly reduced compared with wild-type mice. Levels of BAL eosinophil peroxidase (EPO) were significantly lower in ICAM-1-deficient mice (0.21 \u00b1 0.03 EPO units) compared with wild-type mice (3.34 \u00b1 0.65 EPO units). There was no significant difference in the degree of inhibition of eosinophil recruitment in ICAM-1-deficient mice at the three time points (3, 12, and 24 h) of study after allergen challenge. However, in P-selectin-deficient mice there was a decline in the degree of inhibition of eosinophil recruitment from 3 h (67% inhibition) and 12 h (72% inhibition) postchallenge, to 24 h postchallenge (38% inhibition), suggesting that other adhesion molecules may be playing a more prominent role than P-selectin at later time points. These studies suggest an important role for ICAM-1 and P-selectin in eosinophil recruitment to the lung after allergen challenge.<\/jats:p>","DOI":"10.1165\/ajrcmb.18.2.2829","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T22:13:30Z","timestamp":1365027210000},"page":"218-225","source":"Crossref","is-referenced-by-count":73,"title":["Inhibition of Pulmonary Eosinophilia in P-Selectin- and ICAM-1-deficient Mice"],"prefix":"10.1093","volume":"18","author":[{"given":"David H.","family":"Broide","sequence":"first","affiliation":[{"name":"Department of Medicine, University of California at San Diego, San Diego, California; and Laboratory of Immunology and Vascular Biology, La Jolla Institute for Experimental Medicine, La Jolla, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sue","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California at San Diego, San Diego, California; and Laboratory of Immunology and Vascular Biology, La Jolla Institute for Experimental Medicine, La Jolla, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Gifford","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California at San Diego, San Diego, California; and Laboratory of Immunology and Vascular Biology, La Jolla Institute for Experimental Medicine, La Jolla, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Sriramarao","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California at San Diego, San Diego, California; and Laboratory of Immunology and Vascular Biology, La Jolla Institute for Experimental Medicine, La Jolla, California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1998,2,1]]},"reference":[{"key":"2026030700195113400_B1","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1165\/ajrcmb\/8.4.349","article-title":"Mechanisms of eosinophil recruitment.","volume":"8","author":"Resnick","year":"1993","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026030700195113400_B2","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1182\/blood.V84.7.2068.2068","article-title":"Leukocyte-endothelial adhesion molecules.","volume":"84","author":"Carlos","year":"1994","journal-title":"Blood"},{"key":"2026030700195113400_B3","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1172\/JCI116008","article-title":"Eosinophils express IL-5 and GM-CSF mRNA at sites of allergic inflammation in asthmatics.","volume":"90","author":"Broide","year":"1992","journal-title":"J. Clin. Invest."},{"key":"2026030700195113400_B4","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1084\/jem.173.6.1553","article-title":"Adhesion of human basophils, eosinophils, and neutrophils to IL-1 activated human vascular endothelial cells: contributions of endothelial cell adhesion molecules.","volume":"173","author":"Bochner","year":"1991","journal-title":"J. Exp. Med."},{"key":"2026030700195113400_B5","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1165\/ajrcmb\/7.4.393","article-title":"The expression of leukocyte-endothelial adhesion molecules is increased in perennial allergic rhinitis.","volume":"7","author":"Montefort","year":"1992","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026030700195113400_B6","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1126\/science.1967851","article-title":"Intercellular adhesion molecule-1 in the pathogenesis of asthma.","volume":"247","author":"Wegner","year":"1990","journal-title":"Science"},{"key":"2026030700195113400_B7","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1084\/jem.179.4.1145","article-title":"Role of vascular cell adhesion molecule 1\/very late activation antigen 4 and intercellular adhesion molecule 1\/lymphocyte function-associated antigen 1 interactions in antigen-induced eosinophil and T cell recruitment into the tissue.","volume":"179","author":"Nakajima","year":"1994","journal-title":"J. Exp. Med."},{"key":"2026030700195113400_B8","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1084\/jem.182.4.1169","article-title":"Cooperation between interleukin-5 and the chemokine eotaxin to induce eosinophil accumulation in vivo.","volume":"182","author":"Collins","year":"1995","journal-title":"J. Exp. Med."},{"key":"2026030700195113400_B9","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1172\/JCI118456","article-title":"Cloning of the human eosinophil chemoattractant, eotaxin: expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils.","volume":"97","author":"Ponath","year":"1996","journal-title":"J. Clin. Invest."},{"key":"2026030700195113400_B10","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1111\/j.1749-6632.1996.tb32584.x","article-title":"Differential regulation of eosinophil adhesion under conditions of flow in vivo.","volume":"796","author":"Sriramarao","year":"1996","journal-title":"Ann. N.Y. Acad. Sci."},{"key":"2026030700195113400_B11","doi-asserted-by":"crossref","first-page":"4238","DOI":"10.4049\/jimmunol.153.9.4238","article-title":"L-selectin and very late antigen-4 integrin promote eosinophil rolling at physiological shear rates in vivo.","volume":"153","author":"Sriramarao","year":"1994","journal-title":"J. Immunol."},{"key":"2026030700195113400_B12","doi-asserted-by":"crossref","first-page":"4672","DOI":"10.4049\/jimmunol.157.10.4672","article-title":"E-selectin preferentially supports neutrophil but not eosinophil rolling under conditions of flow in vitro and in vivo.","volume":"157","author":"Sriramarao","year":"1996","journal-title":"J. Immunol."},{"key":"2026030700195113400_B13","first-page":"H1034","article-title":"L-selectin function is required for \u03b22 integrin-mediated neutrophil adhesion at physiological shear rates in vivo.","volume":"263","author":"Von Andrian","year":"1992","journal-title":"Am. J. Physiol."},{"key":"2026030700195113400_B14","doi-asserted-by":"crossref","first-page":"6338","DOI":"10.4049\/jimmunol.151.11.6338","article-title":"Neutrophils roll on E-selectin.","volume":"151","author":"Lawrence","year":"1993","journal-title":"J. Immunol."},{"key":"2026030700195113400_B15","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.4049\/jimmunol.157.4.1711","article-title":"Functional and structural characterization of the eosinophil P-selectin ligand.","volume":"157","author":"Symon","year":"1996","journal-title":"J. Immunol."},{"key":"2026030700195113400_B16","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1083\/jcb.128.4.661","article-title":"P-selectin glycoprotein ligand-1 mediates rolling of human neutrophil on P-selectin.","volume":"128","author":"Moore","year":"1995","journal-title":"J. Cell Biol."},{"key":"2026030700195113400_B17","doi-asserted-by":"crossref","first-page":"774","DOI":"10.4049\/jimmunol.152.2.774","article-title":"Differences between human eosinophils and neutrophils in the function and expression of sialic acid-containing counterligands for E-selectin.","volume":"152","author":"Bochner","year":"1994","journal-title":"J. Immunol."},{"key":"2026030700195113400_B18","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1172\/JCI114175","article-title":"GMP-140, a platelet \u03b1-granule membrane protein, is also synthesized by vascular endothelial cells and is localized in Weibel\u2013Palade bodies.","volume":"84","author":"McEver","year":"1989","journal-title":"J. Clin. Invest."},{"key":"2026030700195113400_B19","doi-asserted-by":"crossref","first-page":"15176","DOI":"10.1016\/S0021-9258(18)42162-X","article-title":"Cloning of the mouse endothelial selectins: expression of both E-selectin and P-selectin is inducible by tumor necrosis factor.","volume":"267","author":"Weller","year":"1992","journal-title":"J. Biol. Chem."},{"key":"2026030700195113400_B20","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1165\/ajrcmb.12.3.7532979","article-title":"Comparison of human eosinophil and neutrophil ligands for P-selectin: ligands for P-selectin differ from those from E-selectin.","volume":"12","author":"Wein","year":"1995","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026030700195113400_B21","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1084\/jem.180.1.371","article-title":"Eosinophil adhesion to nasal polyp endothelium is P-selectin-dependent.","volume":"180","author":"Symon","year":"1994","journal-title":"J. Exp. Med."},{"key":"2026030700195113400_B22","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1016\/0091-6749(92)90218-Q","article-title":"Cytokines in symptomatic asthma airways.","volume":"89","author":"Broide","year":"1992","journal-title":"J. Allergy Clin. Immunol."},{"key":"2026030700195113400_B23","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1172\/JCI117856","article-title":"P-selectin\/ICAM-1 double mutant mice: acute emigration of neutrophils into the peritoneum is completely absent but is normal into pulmonary alveoli.","volume":"95","author":"Bullard","year":"1995","journal-title":"J. Clin. Invest."},{"key":"2026030700195113400_B24","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1172\/JCI117810","article-title":"Adhesion molecule knockouts: one step forward and one step backward.","volume":"95","author":"Ward","year":"1995","journal-title":"J. Clin. Invest."},{"key":"2026030700195113400_B25","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1084\/jem.183.1.195","article-title":"Interleukin-5 deficiency abolishes eosinophilia, airway hyperreactivity, and lung damage in a mouse asthma model.","volume":"183","author":"Foster","year":"1996","journal-title":"J. Exp. Med."},{"key":"2026030700195113400_B26","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/0022-1759(85)90242-X","article-title":"Detection of eosinophils using an eosinophil peroxidase assay: its use as an assay for eosinophil differentiation factors.","volume":"83","author":"Strath","year":"1985","journal-title":"J. Immunol. Methods"},{"key":"2026030700195113400_B27","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1084\/jem.182.1.49","article-title":"Differentiation in vitro of hybrid eosinophil\/basophil granulocytes: autocrine function of an eosinophil developmental intermediate.","volume":"182","author":"Boyce","year":"1995","journal-title":"J. Exp. Med."},{"key":"2026030700195113400_B28","first-page":"2042","article-title":"Passive cutaneous anaphylaxis in mouse skin is associated with local accumulation of IL-6 mRNA and immunoreactive IL-6 protein.","volume":"92","author":"Mican","year":"1992","journal-title":"J. Allergy Clin. Immunol."},{"key":"2026030700195113400_B29","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/0092-8674(95)90491-3","article-title":"Alpha 4 integrins mediate lymphocyte attachment and rolling under physiologic flow.","volume":"80","author":"Berlin","year":"1995","journal-title":"Cell"},{"key":"2026030700195113400_B30","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1084\/jem.184.2.639","article-title":"Selectins and neutrophil traffic: margination and Streptococcus pneumoniae-induced emigration in murine lungs.","volume":"184","author":"Mizgerd","year":"1996","journal-title":"J. Exp. Med."},{"key":"2026030700195113400_B31","doi-asserted-by":"crossref","first-page":"6410","DOI":"10.4049\/jimmunol.151.11.6410","article-title":"Protective effects of selectin chimeras in neutrophil-mediated lung injury.","volume":"151","author":"Mulligan","year":"1993","journal-title":"J. Immunol."},{"key":"2026030700195113400_B32","doi-asserted-by":"crossref","first-page":"5016","DOI":"10.4049\/jimmunol.157.11.5016","article-title":"The roles of CD11\/CD18 and ICAM-1 in acute Pseudomonas aeruginosa-induced pneumonia in mice.","volume":"157","author":"Qin","year":"1996","journal-title":"J. Immunol."},{"key":"2026030700195113400_B33","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1126\/science.1967851","article-title":"Intercellular adhesion molecule-1 in the pathogenesis of asthma.","volume":"247","author":"Wegner","year":"1990","journal-title":"Science"}],"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/18\/2\/218\/67212987\/ajrcmb_18_2_218.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/18\/2\/218\/67212987\/ajrcmb_18_2_218.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T05:28:57Z","timestamp":1772861337000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/18\/2\/218\/8504512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,2,1]]},"references-count":33,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1998,2,1]]},"published-print":{"date-parts":[[1998,2,1]]}},"URL":"https:\/\/doi.org\/10.1165\/ajrcmb.18.2.2829","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1998,2]]},"published":{"date-parts":[[1998,2,1]]}}}