{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:46:02Z","timestamp":1772930762666,"version":"3.50.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1998,4,1]],"date-time":"1998-04-01T00:00:00Z","timestamp":891388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Accumulation of eosinophils in the lung with concomitant tissue damage are defining histopathologic features of human asthma. Through degranulation and the release of proinflammatory proteins such as major basic protein (MBP), eosinophils may perpetuate this inflammatory response. We investigated the extent of eosinophil degranulation in a murine model of allergic pulmonary inflammation. In this paradigm, the mice develop pulmonary eosinophilia, mucus hypersecretion, tissue damage, and airway edema and hyperreactivity. To evaluate the degree of eosinophil degranulation, we used a polyclonal antibody to murine MBP (mMBP) to perform dot blot analysis of bronchoalveolar lavage (BAL) cells and fluids, and immunohistochemical fluorescent analysis of lung tissue sections. After ovalbumin antigen challenge, we were unable to detect immunoreactive mMBP in the BAL fluids from either nonsensitized or sensitized mice. However, after lysis of the recoverable BAL cells, we were able to detect mMBP by immunoblot analysis, with the levels of immunoreactive mMBP directly related to the number of recoverable eosinophils. We also examined paraffin-embedded, lung tissue sections for patterns of mMBP deposition. Whereas lung sections from allergic mice revealed prominent peribronchial eosinophilia after antigen challenge, tissue sections from nonsensitized animals rarely displayed eosinophils. Despite the presence of numerous eosinophils, no immunohistologic evidence of extracellular mMBP could be found in antigen-challenged allergic mice. Furthermore, rechallenged allergic mice displayed a significant increase in the number of recruited pulmonary eosinophils but all immunoreactive mMBP was still intracellular. We conclude that the recruited pulmonary eosinophils have not substantially degranulated. These results suggest that, in this murine model of allergic inflammation, eosinophil degranulation and release of mMBP does not contribute to the observed pulmonary inflammation and airway hyperreactivity.<\/jats:p>","DOI":"10.1165\/ajrcmb.18.4.2957","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T22:11:11Z","timestamp":1365027071000},"page":"463-470","source":"Crossref","is-referenced-by-count":55,"title":["Eosinophils Retain Their Granule Major Basic Protein in a Murine Model of Allergic Pulmonary Inflammation"],"prefix":"10.1093","volume":"18","author":[{"given":"D.","family":"Stelts","sequence":"first","affiliation":[{"name":"Departments of Immunology and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota; and Schering Plough Research Institute, Department of Allergy and Immunology, Kenilworth, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. W.","family":"Egan","sequence":"additional","affiliation":[{"name":"Departments of Immunology and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota; and Schering Plough Research Institute, Department of Allergy and Immunology, Kenilworth, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Falcone","sequence":"additional","affiliation":[{"name":"Departments of Immunology and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota; and Schering Plough Research Institute, Department of Allergy and Immunology, Kenilworth, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. G.","family":"Garlisi","sequence":"additional","affiliation":[{"name":"Departments of Immunology and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota; and Schering Plough Research Institute, Department of Allergy and Immunology, Kenilworth, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. J.","family":"Gleich","sequence":"additional","affiliation":[{"name":"Departments of Immunology and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota; and Schering Plough Research Institute, Department of Allergy and Immunology, Kenilworth, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Kreutner","sequence":"additional","affiliation":[{"name":"Departments of Immunology and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota; and Schering Plough Research Institute, Department of Allergy and Immunology, Kenilworth, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. T.","family":"Kung","sequence":"additional","affiliation":[{"name":"Departments of Immunology and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota; and Schering Plough Research Institute, Department of Allergy and Immunology, Kenilworth, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. K.","family":"Nahrebne","sequence":"additional","affiliation":[{"name":"Departments of Immunology and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota; and Schering Plough Research Institute, Department of Allergy and Immunology, Kenilworth, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. W.","family":"Chapman","sequence":"additional","affiliation":[{"name":"Departments of Immunology and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota; and Schering Plough Research Institute, Department of Allergy and Immunology, Kenilworth, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Minnicozzi","sequence":"additional","affiliation":[{"name":"Departments of Immunology and Internal Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota; and Schering Plough Research Institute, Department of Allergy and Immunology, Kenilworth, New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1998,4,1]]},"reference":[{"key":"2026030700192241700_B1","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1183\/09031936.93.06060840","article-title":"Dynamics of eosinophil infiltration in the bronchial mucosa before and after the late asthmatic reaction.","volume":"6","author":"Aalbers","year":"1993","journal-title":"Eur. Respir. J."},{"key":"2026030700192241700_B2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0065-2776(08)60491-5","article-title":"The pathobiology of bronchial asthma.","volume":"51","author":"Am","year":"1992","journal-title":"Adv. Immunol."},{"key":"2026030700192241700_B3","doi-asserted-by":"crossref","first-page":"2813","DOI":"10.1172\/JCI117986","article-title":"Allergen-specific IgG1 and IgG3 through FcRII induce eosinophil degranulation.","volume":"95","author":"Kaneko","year":"1995","journal-title":"J. Clin. Invest."},{"key":"2026030700192241700_B4","doi-asserted-by":"crossref","first-page":"3518","DOI":"10.4049\/jimmunol.142.10.3518","article-title":"Stimulation of degranulation from human eosinophils by platelet activating factor.","volume":"142","author":"Kroegel","year":"1989","journal-title":"J. Immunol."},{"key":"2026030700192241700_B5","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1002\/jlb.52.6.611","article-title":"Eosinophil granule proteins in peripheral blood granulocytes.","volume":"52","author":"Abu-Ghazaleh","year":"1992","journal-title":"J. Leuk. Biol."},{"key":"2026030700192241700_B6","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/0091-6749(86)90341-6","article-title":"The eosinophil and pathophysiology of asthma.","volume":"77","author":"Frigas","year":"1986","journal-title":"J. Allergy Clin. Immunol."},{"key":"2026030700192241700_B7"},{"key":"2026030700192241700_B8","doi-asserted-by":"crossref","first-page":"S66","DOI":"10.1164\/ajrccm\/143.3_Pt_2.S66","article-title":"The role of the eosinophil in asthma.","volume":"143","author":"Holgate","year":"1991","journal-title":"Am. Rev. Respir. Dis."},{"key":"2026030700192241700_B9","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1164\/ajrccm\/147.4.982","article-title":"The effects of human eosinophil granule major basic protein on rat airways responsiveness in vivo: a comparison with polycations.","volume":"147","author":"Urchida","year":"1993","journal-title":"Am. Rev. Respir. Dis."},{"key":"2026030700192241700_B10","first-page":"35","article-title":"Cytotoxic effects of the guinea pig eosinophil major basic protein on tracheal epithelium.","volume":"42","author":"Frigas","year":"1980","journal-title":"Lab. Invest."},{"key":"2026030700192241700_B11","doi-asserted-by":"crossref","first-page":"2664","DOI":"10.4049\/jimmunol.153.6.2664","article-title":"Eosinophil granule proteins increase microvascular macromolecular transport in the hamster cheek pouch.","volume":"153","author":"Minnicozzi","year":"1994","journal-title":"J. Immunol."},{"key":"2026030700192241700_B12","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1159\/000236807","article-title":"Characterization of a murine model of allergic pulmonary inflammation.","volume":"105","author":"Kung","year":"1994","journal-title":"Int. Arch. Aller. Immunol."},{"key":"2026030700192241700_B13","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1165\/ajrcmb.10.6.8003337","article-title":"Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia.","volume":"10","author":"Gavett","year":"1994","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026030700192241700_B14","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/0926-6917(95)90061-6","article-title":"Bronchoconstriction and airway hyperresponsiveness after ovalbumin inhalation in sensitized mice.","volume":"293","author":"Hessel","year":"1995","journal-title":"Eur. J. Pharmacol."},{"key":"2026030700192241700_B15","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1164\/ajrccm\/137.4.948","article-title":"Eosinophil recruitment into the guinea pig lungs after PAF-acether and allergen administration.","volume":"137","author":"Lellouch-Tubiana","year":"1988","journal-title":"Am. Rev. Respir. Dis."},{"key":"2026030700192241700_B16","doi-asserted-by":"crossref","first-page":"181","DOI":"10.3109\/02770909209099026","article-title":"Asthma: cells and cytokines.","volume":"29","author":"Litchfield","year":"1992","journal-title":"J. Asthma"},{"key":"2026030700192241700_B17","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1165\/ajrcmb.13.3.7654390","article-title":"Involvement of IL-5 in a murine model of allergic pulmonary inflammation: prophylactic and therapeutic effect of an anti-IL-5 antibody.","volume":"13","author":"Kung","year":"1995","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026030700192241700_B18","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1165\/ajrcmb.16.3.9070605","article-title":"Infiltration of eosinophils into the lung of asthmatic airways caused by interleukin 5.","volume":"16","author":"Shi","year":"1997","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026030700192241700_B19","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1164\/ajrccm\/147.4.901","article-title":"IL-5 is the predominant eosinophil active cytokine in the antigen-induced pulmonary late phase reaction.","volume":"147","author":"Ohnishi","year":"1993","journal-title":"Am. Rev. Respir. Dis."},{"key":"2026030700192241700_B20","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1165\/ajrcmb.17.5.2866","article-title":"Airway eosinophils, T cells, Th2-type cytokine mRNA and hyperreactivity in response to aerosol challenge of allergic mice with previously established pulmonary inflammation.","volume":"17","author":"Garlisi","year":"1997","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026030700192241700_B21"},{"key":"2026030700192241700_B22"},{"key":"2026030700192241700_B23","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/0022-1759(81)90123-X","article-title":"An immunofluorescent method for specific staining of eosinophil granule major basic protein.","volume":"47","author":"Filley","year":"1981","journal-title":"J. Immunol. 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