{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:43:57Z","timestamp":1772930637725,"version":"3.50.1"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1998,10,1]],"date-time":"1998-10-01T00:00:00Z","timestamp":907200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In the present study, we investigated whether allergen immunotherapy is effective in a murine model with immunologic and pathophysiologic features reminiscent of allergic asthma. Ovalbumin-sensitized mice received increasing (1 \u03bcg to 1 mg) subcutaneous doses of ovalbumin twice a week for 8 wk according to a semirush immunotherapy protocol as used in allergic patients. During immunotherapy, an initial rise in serum levels of ovalbumin-specific antibodies (immunoglobulin [Ig]G1, IgE, IgG2a) occurred, after which IgE levels decreased sharply concomitant with an increase in IgG2a levels. The increase in IgG2a levels, with the decline in IgE levels, suggests that during immunotherapy interferon-\u03b3 production is increased or interleukin (IL)-4 production is decreased. After immunotherapy, inhalation challenge of the mice with ovalbumin revealed almost complete inhibition (98%, P &amp;lt; 0.01) of eosinophil infiltration into bronchoalveolar lavage and airway hyperresponsiveness (100% at 320 \u03bcg\/kg methacholine, P &amp;lt; 0.05) compared with sham-treated animals. In addition, IL-4 production of thoracic lymph node cells stimulated with ovalbumin in vitro was largely reduced (60%, P &amp;lt; 0.05) after immunotherapy. Thus, effective immunotherapy in this animal model appears to be due to modulation of antigen-specific T cells. Similar effects on airway symptoms and IL-4 production can be obtained within 1 wk by three injections of the highest dose of ovalbumin (1 mg). This animal model will be used as a preclinical model to improve allergen immunotherapy and to gain more insight into the mechanisms involved.<\/jats:p>","DOI":"10.1165\/ajrcmb.19.4.3112m","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T22:21:45Z","timestamp":1365027705000},"page":"622-628","source":"Crossref","is-referenced-by-count":34,"title":["Allergen Immunotherapy Inhibits Airway Eosinophilia and Hyperresponsiveness Associated with Decreased IL-4 Production by Lymphocytes in a Murine Model of Allergic Asthma"],"prefix":"10.1093","volume":"19","author":[{"given":"Antoon J. M.","family":"Van Oosterhout","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht University, Utrecht; Laboratory of Cell Biology and Histology, Academic Medical Center, Amsterdam; Department of Immunology, Erasmus University, Rotterdam; and Department of Pulmonology, Academic Medical Center, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Betty","family":"Van Esch","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht University, Utrecht; Laboratory of Cell Biology and Histology, Academic Medical Center, Amsterdam; Department of Immunology, Erasmus University, Rotterdam; and Department of Pulmonology, Academic Medical Center, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerard","family":"Hofman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht University, Utrecht; Laboratory of Cell Biology and Histology, Academic Medical Center, Amsterdam; Department of Immunology, Erasmus University, Rotterdam; and Department of Pulmonology, Academic Medical Center, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia L.","family":"Hofstra","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht University, Utrecht; Laboratory of Cell Biology and Histology, Academic Medical Center, Amsterdam; Department of Immunology, Erasmus University, Rotterdam; and Department of Pulmonology, Academic Medical Center, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid","family":"Van Ark","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht University, Utrecht; Laboratory of Cell Biology and Histology, Academic Medical Center, Amsterdam; Department of Immunology, Erasmus University, Rotterdam; and Department of Pulmonology, Academic Medical Center, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frans P.","family":"Nijkamp","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht University, Utrecht; Laboratory of Cell Biology and Histology, Academic Medical Center, Amsterdam; Department of Immunology, Erasmus University, Rotterdam; and Department of Pulmonology, Academic Medical Center, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martien L.","family":"Kapsenberg","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht University, Utrecht; Laboratory of Cell Biology and Histology, Academic Medical Center, Amsterdam; Department of Immunology, Erasmus University, Rotterdam; and Department of Pulmonology, Academic Medical Center, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huub F. J.","family":"Savelkoul","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht University, Utrecht; Laboratory of Cell Biology and Histology, Academic Medical Center, Amsterdam; Department of Immunology, Erasmus University, Rotterdam; and Department of Pulmonology, Academic Medical Center, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank R.","family":"Weller","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht University, Utrecht; Laboratory of Cell Biology and Histology, Academic Medical Center, Amsterdam; Department of Immunology, Erasmus University, Rotterdam; and Department of Pulmonology, Academic Medical Center, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1998,10,1]]},"reference":[{"key":"2026030700161709300_B1","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1016\/S0140-6736(00)78276-6","article-title":"Prophylactic inoculation against hay fever.","volume":"1","author":"Noon","year":"1911","journal-title":"Lancet"},{"key":"2026030700161709300_B2","first-page":"969","article-title":"Is allergen immunotherapy effective in asthma? 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