{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:29:17Z","timestamp":1791188957637,"version":"4.1.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1999,4,1]],"date-time":"1999-04-01T00:00:00Z","timestamp":922924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1999,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Hypoxic pulmonary vasoconstriction (HPVC) is mediated, in part, via membrane depolarization and inhibition of K+ channels. We recently observed that the naturally occurring steroid dehydroepiandrosterone (DHEA) reversed and prevented HPVC in isolated perfused and ventilated ferret lungs. In the current study, we investigated the effects of DHEA on the major K+ channels of chronically hypoxic human pulmonary smooth-muscle cells (HPSMC). K+ channels were recorded by using the patch-clamp technique in whole-cell and single-channel configurations. Single-channel recordings were performed in inside-out and outside-out excised patches, and in intact HPSMC in cell-attached configuration. Using whole-cell current recording, chronic hypoxia decreased the high-amplitude, high-noise, and charybdotoxin-sensitive Ca2+-dependent K+ channels (KCa). DHEA reversed the effect of chronic hypoxia on KCa, but had no effect on the low-amplitude, low-noise, and 4-aminopyridine\u2013sensitive delayed rectifying K+ channels. In the cell-attached configuration, chronic hypoxia caused a decrease in KCa sensitivity to membrane potential (Em). DHEA reversed the effect of hypoxia on KCa sensitivity to Em and caused a mean of 40-mV left shift in voltage-dependent activation of KCa. DHEA increased KCa activation from both sides of membrane patches of hypoxic HPSMC via a cyclic adenosine monophosphate\u2013and cyclic guanosine monophosphate\u2013independent pathway. We concluded that DHEA is a novel KCa opener of the human pulmonary vasculature.<\/jats:p>","DOI":"10.1165\/ajrcmb.20.4.3390","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T21:59:27Z","timestamp":1365026367000},"page":"737-745","source":"Crossref","is-referenced-by-count":41,"title":["Role of a Novel KCa Opener in Regulating K+ Channels of Hypoxic Human Pulmonary Vascular Cells"],"prefix":"10.1093","volume":"20","author":[{"given":"Wei","family":"Peng","sequence":"first","affiliation":[{"name":"Division of Respiratory, Critical Care and Occupational Medicine, Department of Internal Medicine, University of Utah Health Sciences Center, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R.","family":"Hoidal","sequence":"additional","affiliation":[{"name":"Division of Respiratory, Critical Care and Occupational Medicine, Department of Internal Medicine, University of Utah Health Sciences Center, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imad S.","family":"Farrukh","sequence":"additional","affiliation":[{"name":"Division of Respiratory, Critical Care and Occupational Medicine, Department of Internal Medicine, University of Utah Health Sciences Center, Salt Lake City, Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1999,4,1]]},"reference":[{"key":"2026030700130163200_B1","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1152\/jappl.1985.59.5.1389","article-title":"Hypoxic induction of Ca2+-dependent action potentials in small pulmonary arteries of the cat.","volume":"59","author":"Harder","year":"1985","journal-title":"J. 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