{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T12:46:28Z","timestamp":1789821988996,"version":"4.0.1"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The role of lymphocytes bearing \u03b1\u03b2 or \u03b3\u03b4 T-cell receptors (TCRs) was assessed during the acute allergic response in a mouse model of asthma. The inflammatory immune response to ovalbumin (OVA) was characterized in wild-type C57BL\/6J mice and congenic TCR\u03b2\u2212\/\u2212 and TCR\u03b4\u2212\/\u2212 mice by evaluation of airway eosinophilia, histopathology, serum immunoglobulin (Ig)E levels, and in vivo airway responsiveness to methacholine. OVA-challenged wild-type mice demonstrated marked pulmonary inflammation, evidenced by airway eosinophilia (68 \u00b1 7 \u00d7 104 cells), peribronchial lympho-plasmocytic infiltration, and elevated serum IgE (4.9 \u00b1 0.6 \u03bcg\/ml). These responses were markedly attenuated in TCR\u03b4\u2212\/\u2212 animals (5.0 \u00b1 1.0 \u00d7 104 eosinophils and 1.6 \u00b1 0.3 \u03bcg\/ml IgE) and were completely absent in TCR\u03b2\u2212\/\u2212 mice (&amp;lt; 1 \u00d7 103 eosinophils and 0.38 \u00b1 0.21 \u03bcg\/ml IgE). Similar results were observed in mice treated with anti-TCR\u03b3\u03b4 or anti-TCR\u03b1\u03b2 monoclonal antibodies. Airway responsiveness to aerosolized methacholine was also reduced in challenged TCR\u03b4\u2212\/\u2212 animals relative to challenged wild-type mice. These results demonstrate that acute allergic airway responses are dependent upon intact TCR\u03b1\u03b2 and TCR\u03b3\u03b4 lymphocyte function and that TCR\u03b3\u03b4 cells promote acute airway sensitization.<\/jats:p>","DOI":"10.1165\/ajrcmb.22.2.3620","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T21:44:45Z","timestamp":1365025485000},"page":"218-225","source":"Crossref","is-referenced-by-count":55,"title":["Proinflammatory Roles of T-Cell Receptor (TCR) \u03b3\u03b4 and TCR \u03b1\u03b2 Lymphocytes in a Murine Model of Asthma"],"prefix":"10.1093","volume":"22","author":[{"given":"Craig M.","family":"Schramm","sequence":"first","affiliation":[{"name":"Departments of Pediatrics and Medicine, University of Connecticut School of Medicine, Farmington; Department of Biology, Capital Community Technical College, Hartford; Department of Pathobiology, University of Connecticut, Storrs; and Boehringer-Ingelheim Pharmaceuticals, Incorporated, Ridgefield, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn","family":"Puddington","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Medicine, University of Connecticut School of Medicine, Farmington; Department of Biology, Capital Community Technical College, Hartford; Department of Pathobiology, University of Connecticut, Storrs; and Boehringer-Ingelheim Pharmaceuticals, Incorporated, Ridgefield, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen A.","family":"Yiamouyiannis","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Medicine, University of Connecticut School of Medicine, Farmington; Department of Biology, Capital Community Technical College, Hartford; Department of Pathobiology, University of Connecticut, Storrs; and Boehringer-Ingelheim Pharmaceuticals, Incorporated, Ridgefield, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth G.","family":"Lingenheld","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Medicine, University of Connecticut School of Medicine, Farmington; Department of Biology, Capital Community Technical College, Hartford; Department of Pathobiology, University of Connecticut, Storrs; and Boehringer-Ingelheim Pharmaceuticals, Incorporated, Ridgefield, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert E.","family":"Whiteley","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Medicine, University of Connecticut School of Medicine, Farmington; Department of Biology, Capital Community Technical College, Hartford; Department of Pathobiology, University of Connecticut, Storrs; and Boehringer-Ingelheim Pharmaceuticals, Incorporated, Ridgefield, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter W.","family":"Wolyniec","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Medicine, University of Connecticut School of Medicine, Farmington; Department of Biology, Capital Community Technical College, Hartford; Department of Pathobiology, University of Connecticut, Storrs; and Boehringer-Ingelheim Pharmaceuticals, Incorporated, Ridgefield, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C.","family":"Noonan","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Medicine, University of Connecticut School of Medicine, Farmington; Department of Biology, Capital Community Technical College, Hartford; Department of Pathobiology, University of Connecticut, Storrs; and Boehringer-Ingelheim Pharmaceuticals, Incorporated, Ridgefield, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger S.","family":"Thrall","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Medicine, University of Connecticut School of Medicine, Farmington; Department of Biology, Capital Community Technical College, Hartford; Department of Pathobiology, University of Connecticut, Storrs; and Boehringer-Ingelheim Pharmaceuticals, Incorporated, Ridgefield, Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,2,1]]},"reference":[{"key":"2026030700071416500_B1","doi-asserted-by":"crossref","first-page":"1928","DOI":"10.1056\/NEJM199212313272708","article-title":"Asthma.","volume":"327","author":"McFadden","year":"1992","journal-title":"N. Engl. J. Med."},{"key":"2026030700071416500_B2","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1093\/oxfordjournals.bmb.a072543","article-title":"Asthma: role of T-lymphocytes and lymphokines.","volume":"48","author":"Corrigan","year":"1992","journal-title":"Br. Med. Bull."},{"key":"2026030700071416500_B3","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/0091-6749(91)90251-I","article-title":"Activated T cells and eosinophilia in bronchoalveolar lavages from subjects with asthma correlated with disease severity.","volume":"88","author":"Walker","year":"1991","journal-title":"J. Allergy Clin. Immunol."},{"key":"2026030700071416500_B4","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/0167-5699(92)90026-4","article-title":"T cells and eosinophils in the pathogenesis of asthma.","volume":"13","author":"Corrigan","year":"1992","journal-title":"Immunol. Today"},{"key":"2026030700071416500_B5","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1164\/ajrccm\/145.4_Pt_1.958","article-title":"Lymphocyte activation in bronchoalveolar lavage and peripheral blood in atopic asthma.","volume":"145","author":"Wilson","year":"1992","journal-title":"Am. Rev. Respir. Dis."},{"key":"2026030700071416500_B6","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1056\/NEJM199201303260504","article-title":"Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma.","volume":"326","author":"Robinson","year":"1992","journal-title":"N. Engl. J. Med."},{"key":"2026030700071416500_B7","doi-asserted-by":"crossref","first-page":"949","DOI":"10.4049\/jimmunol.136.3.949","article-title":"A T cell activity that enhances polyclonal IgE production and its inhibition by interferon-\u03b3.","volume":"136","author":"Coffman","year":"1986","journal-title":"J. Immunol."},{"key":"2026030700071416500_B8","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/BF00916934","article-title":"Regulation of IgE synthesis in humans.","volume":"9","author":"Vercelli","year":"1989","journal-title":"J. Clin. Immunol."},{"key":"2026030700071416500_B9","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1084\/jem.179.1.135","article-title":"Interleukin 13 is a B cell stimulating factor.","volume":"179","author":"Defrance","year":"1994","journal-title":"J. Exp. Med."},{"key":"2026030700071416500_B10","doi-asserted-by":"crossref","first-page":"S22","DOI":"10.1164\/ajrccm\/145.2_Pt_2.S22","article-title":"\u201cHelper\u201d (CD4+) T cells and eosinophils in allergy and asthma.","volume":"145","author":"Kay","year":"1992","journal-title":"Am. Rev. Respir. Dis."},{"key":"2026030700071416500_B11","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1164\/ajrccm\/146.2.374","article-title":"CD4+ T-lymphocytes and interleukin-5 mediate antigen-induced eosinophil infiltration into the mouse trachea.","volume":"146","author":"Nakajima","year":"1992","journal-title":"Am. Rev. Respir. Dis."},{"key":"2026030700071416500_B12","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1006\/clin.1996.0194","article-title":"Characterization of the antigen-presenting cell and T cell requirements for induction of pulmonary eosinophilia in a murine model of asthma.","volume":"81","author":"Lambert","year":"1996","journal-title":"Clin. Immunol. Immunopathol."},{"key":"2026030700071416500_B13","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1165\/ajrcmb.10.6.8003337","article-title":"Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia.","volume":"10","author":"Gavett","year":"1994","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026030700071416500_B14","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1136\/jcp.46.10.926","article-title":"Distribution of \u03b3\/\u03b4 T cells in the bronchial tree of smokers and non-smokers.","volume":"46","author":"Richmond","year":"1993","journal-title":"J. Clin. Pathol."},{"key":"2026030700071416500_B15","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/340239a0","article-title":"Resident pulmonary lymphocytes expressing the \u03b3\/\u03b4 T cell receptor.","volume":"340","author":"Augustin","year":"1989","journal-title":"Nature"},{"key":"2026030700071416500_B16","doi-asserted-by":"crossref","first-page":"2272","DOI":"10.1073\/pnas.93.6.2272","article-title":"\u03b3\/\u03b4 and other unconventional T lymphocytes: what do they see and what do they do?","volume":"93","author":"Kaufmann","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026030700071416500_B17","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1164\/ajrccm.153.5.8630617","article-title":"Phenotypic and molecular characteristics of nasal mucosal \u03b3\/\u03b4 T cells in allergic and infectious rhinitis.","volume":"153","author":"Pawankar","year":"1996","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"2026030700071416500_B18","doi-asserted-by":"crossref","first-page":"223","DOI":"10.7326\/0003-4819-124-2-199601150-00005","article-title":"Increased allergen specific, steroid sensitive \u03b3\u03b4 T cells in bronchoalveolar lavage from patients with asthma.","volume":"124","author":"Spinozzi","year":"1996","journal-title":"Ann. Intern. Med."},{"key":"2026030700071416500_B19","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1126\/science.7916481","article-title":"Regulation of IgE responses to inhaled antigen in mice by antigen-specific \u03b3\/\u03b4 T cells.","volume":"265","author":"McMenamin","year":"1994","journal-title":"Science"},{"key":"2026030700071416500_B20","doi-asserted-by":"crossref","first-page":"4390","DOI":"10.4049\/jimmunol.154.9.4390","article-title":"\u03b3\/\u03b4 T cells down regulate primary IgE responses in rats to inhaled soluble protein antigens.","volume":"154","author":"McMenamin","year":"1995","journal-title":"J. Immunol."},{"key":"2026030700071416500_B21","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1126\/science.280.5367.1265","article-title":"Requirement for \u03b3\u03b4 T cells in allergic airway inflammation.","volume":"280","author":"Zuany-Amorim","year":"1998","journal-title":"Science"},{"key":"2026030700071416500_B22","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1073\/pnas.88.8.3084","article-title":"Creation of a large genomic deletion at the T-cell antigen receptor \u03b2-subunit locus in mouse embryonic stem cells by gene targeting.","volume":"88","author":"Mombaerts","year":"1991","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026030700071416500_B23","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/0092-8674(93)90112-4","article-title":"T cell receptor \u03b4 gene mutant mice: independent generation of \u03b1\u03b2 T cells and programmed rearrangement of \u03b3\u03b4 TCR genes.","volume":"72","author":"Itohara","year":"1993","journal-title":"Cell"},{"key":"2026030700071416500_B24","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1159\/000236807","article-title":"Characterization of a murine model of allergic pulmonary inflammation.","volume":"105","author":"Kung","year":"1994","journal-title":"Int. Arch Allergy Immunol."},{"key":"2026030700071416500_B25","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1165\/ajrcmb.16.5.9160833","article-title":"Cytokine and eosinophil responses in the lung, peripheral blood, and bone marrow compartments in a murine model of allergen-induced airways inflammation.","volume":"16","author":"Ohkawara","year":"1997","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026030700071416500_B26","doi-asserted-by":"crossref","first-page":"2736","DOI":"10.4049\/jimmunol.142.8.2736","article-title":"Characterization of a monoclonal antibody which detects all murine \u03b1\u03b2 T cell receptors.","volume":"142","author":"Kubo","year":"1989","journal-title":"J. Immunol."},{"key":"2026030700071416500_B27","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1084\/jem.170.5.1569","article-title":"Intraepithelial lymphocytes: anatomical site, not T cell receptor form, dictates phenotype and function.","volume":"170","author":"Goodman","year":"1989","journal-title":"J. Exp. Med."},{"key":"2026030700071416500_B28","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent.","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"2026030700071416500_B29","first-page":"488","article-title":"Differential cellular analysis of bronchoalveolar lavage fluid obtained at various stages during the development of bleomycin-induced pulmonary fibrosis in the rat.","volume":"126","author":"Thrall","year":"1982","journal-title":"Am. Rev. Respir. Dis."},{"key":"2026030700071416500_B30","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/0091-6749(92)90296-E","article-title":"Aerosolized antigen exposure without adjuvant causes increased IgE production and increased airway responsiveness in the mouse.","volume":"89","author":"Renz","year":"1992","journal-title":"J. Allergy Clin. Immunol."},{"key":"2026030700071416500_B31","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1165\/ajrcmb.18.6.3056","article-title":"Reduction of antigen-induced airway hyperreactivity and eosinophilia in ICAM-1-deficient mice.","volume":"18","author":"Wolyniec","year":"1998","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026030700071416500_B32","doi-asserted-by":"crossref","first-page":"2148","DOI":"10.1152\/jappl.1995.79.6.2148","article-title":"Methacholine induced pulmonary gas trapping in the guinea pig, hamster, mouse and rat.","volume":"79","author":"Stengel","year":"1995","journal-title":"J. Appl. Physiol."},{"key":"2026030700071416500_B33","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1016\/S0022-3565(25)24126-X","article-title":"Reversal of A23187-induced airway constriction in the guinea pig.","volume":"248","author":"Stengel","year":"1989","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"2026030700071416500_B34","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1038\/sj.bjp.0700860","article-title":"Inhibition by the immunosuppressive agent FK-506 of antigen- induced airways eosinophilia and bronchial hyperreactivity in mice.","volume":"120","author":"Eum","year":"1997","journal-title":"Br. J. Pharmacol."},{"key":"2026030700071416500_B35","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1093\/intimm\/6.7.1061","article-title":"Peripheral lymphoid development and function in TCR mutant mice.","volume":"6","author":"Mombaerts","year":"1994","journal-title":"Int. Immunol."},{"key":"2026030700071416500_B36","doi-asserted-by":"crossref","first-page":"2271","DOI":"10.1084\/jem.183.5.2271","article-title":"Germinal center formation, immunoglobulin class switching, and autoantibody production driven by \u201cnon \u03b1\/\u03b2\u201d T cells.","volume":"183","author":"Wen","year":"1996","journal-title":"J. Exp. Med."},{"key":"2026030700071416500_B37","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1084\/jem.187.5.721","article-title":"Aerosol- induced immunoglobulin (Ig)-E unresponsiveness to ovalbumin does not require CD8+ or T cell receptor (TCR)-\u03b3\u03b4+ T cells or interferon (IFN)-\u03b3 in a murine model of allergic sensitization.","volume":"187","author":"Seymour","year":"1998","journal-title":"J. Exp. Med."},{"key":"2026030700071416500_B38","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/373255a0","article-title":"Differential production of interferon-\u03b3 and interleukin-4 in response to Th1 and Th2 stimulating pathogens by \u03b3\/\u03b4 T cells in vivo.","volume":"373","author":"Ferrick","year":"1995","journal-title":"Nature"},{"key":"2026030700071416500_B39","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.4049\/jimmunol.160.4.1965","article-title":"Primary \u03b3\u03b4 cell clones can be defined phenotypically and functionally as Th1\/Th2 cells and illustrate the association of CD4 with Th2 differentiation.","volume":"160","author":"Wen","year":"1998","journal-title":"J. Immunol."},{"key":"2026030700071416500_B40","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1002\/jlb.63.6.707","article-title":"\u03b3\u03b4 T cells present antigen to CD4+ \u03b1\u03b2 T cells.","volume":"63","author":"Collins","year":"1998","journal-title":"J. Leukoc. Biol."}],"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/22\/2\/218\/67191589\/ajrcmb_22_2_218.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/22\/2\/218\/67191589\/ajrcmb_22_2_218.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T05:18:24Z","timestamp":1772860704000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/22\/2\/218\/8502804"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,2,1]]},"references-count":40,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2000,2,1]]},"published-print":{"date-parts":[[2000,2,1]]}},"URL":"https:\/\/doi.org\/10.1165\/ajrcmb.22.2.3620","relation":{},"ISSN":["1535-4989","1044-1549"],"issn-type":[{"value":"1535-4989","type":"electronic"},{"value":"1044-1549","type":"print"}],"subject":[],"published-other":{"date-parts":[[2000,2]]},"published":{"date-parts":[[2000,2,1]]}}}