{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:33:44Z","timestamp":1772930024023,"version":"3.50.1"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2001,5,1]],"date-time":"2001-05-01T00:00:00Z","timestamp":988675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Rhinovirus (RV) is a major cause of wheezing in asthmatics and has been reported to cause \u03b22 adrenergic receptor hyporesponsiveness in human airway smooth muscle (HASM) via cellular secretion of interleukin (IL)-1 \u03b2. We studied the effects of IL-1 \u03b2 and RV on cyclic adenosine monophosphate (cAMP) production in HASM cells. Chronic incubation with IL-1 \u03b2 or RV caused a significant increase ( \u223c 3- and \u223c 2-fold, respectively) in forskolin (FSK)-stimulated cAMP production, suggesting a sensitization of adenylyl cyclase (AC). The observed augmentation of FSK-stimulated cAMP formation by IL-1 \u03b2 was completely abrogated by pretreatment with an IL-1 receptor antagonist or cycloheximide, demonstrating that the effect is mediated via the IL-1 receptor 1 (IL-1R1) and that de novo protein synthesis is required. In contrast, RV-induced AC sensitization was not mediated via the IL-1R1 but was observed to be protein kinase C\u2013dependent. We suggest that the sensitization of AC observed after exposure to IL-1 \u03b2 or RV infection is a cellular defense mechanism to promote pathways that induce relaxation in the inflamed airway.<\/jats:p>","DOI":"10.1165\/ajrcmb.24.5.4215","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T21:26:44Z","timestamp":1365024404000},"page":"633-639","source":"Crossref","is-referenced-by-count":22,"title":["Interleukin-1 \u03b2 and Rhinovirus Sensitize Adenylyl Cyclase in Human Airway Smooth-Muscle Cells"],"prefix":"10.1093","volume":"24","author":[{"given":"Charlotte K.","family":"Billington","sequence":"first","affiliation":[{"name":"Division of Therapeutics & Institute of Cell Signalling, and Public Health Laboratory, University Hospital of Nottingham, Nottingham, United Kingdom; and Departments of Medicine, Division of Critical Care, Pulmonary, Allergic, and Immunological Diseases, and Microbiology & Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodolfo M.","family":"Pascual","sequence":"additional","affiliation":[{"name":"Division of Therapeutics & Institute of Cell Signalling, and Public Health Laboratory, University Hospital of Nottingham, Nottingham, United Kingdom; and Departments of Medicine, Division of Critical Care, Pulmonary, Allergic, and Immunological Diseases, and Microbiology & Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael L.","family":"Hawkins","sequence":"additional","affiliation":[{"name":"Division of Therapeutics & Institute of Cell Signalling, and Public Health Laboratory, University Hospital of Nottingham, Nottingham, United Kingdom; and Departments of Medicine, Division of Critical Care, Pulmonary, Allergic, and Immunological Diseases, and Microbiology & Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond B.","family":"Penn","sequence":"additional","affiliation":[{"name":"Division of Therapeutics & Institute of Cell Signalling, and Public Health Laboratory, University Hospital of Nottingham, Nottingham, United Kingdom; and Departments of Medicine, Division of Critical Care, Pulmonary, Allergic, and Immunological Diseases, and Microbiology & Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian P.","family":"Hall","sequence":"additional","affiliation":[{"name":"Division of Therapeutics & Institute of Cell Signalling, and Public Health Laboratory, University Hospital of Nottingham, Nottingham, United Kingdom; and Departments of Medicine, Division of Critical Care, Pulmonary, Allergic, and Immunological Diseases, and Microbiology & Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,5,1]]},"reference":[{"key":"2026030700024214400_B1","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1164\/ajrccm.152.3.7663795","article-title":"Beta-adrenergic receptors and their regulation.","volume":"152","author":"Barnes","year":"1995","journal-title":"Am. 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