{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:55:59Z","timestamp":1782284159717,"version":"3.54.5"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2001,9,1]],"date-time":"2001-09-01T00:00:00Z","timestamp":999302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Small pulmonary arteries are the major determinants of pulmonary artery pressure and vascular resistance. Their endothelium modulates pulmonary resistance, remodeling, and blood fluidity. We developed a method that provides access to the luminal surface of small pulmonary arteries of rat and allows the patch-clamp study of electrical properties of in situ endothelium. At birth, the membrane was predominantly permeable for K+, showing a resting potential of \u2212 70 mV. This conductance was not voltage-dependent and was insensitive to standard blockers of K+ channels such as tetraethylammonium, charybdotoxin, and 4-aminopyridine. The first 22 d of development were accompanied by an additional expression of a Cl\u2212 conductance, increasing membrane potential to \u2212 45 mV. Acidosis reduced K+ conductance and depolarized the membrane, whereas alkalosis resulted in hyperpolarization. Two-electrode recordings revealed tight electrical coupling (83%) between neighboring cells in the circumferential direction of the artery. The electrotonic length constant for endothelium was 13.3 \u03bc m, indicating that most cells in one cross section of a small artery are well coupled. Thus, the resting membrane conductances in small pulmonary artery endothelial cells change with postnatal development and are modulated by pH.<\/jats:p>","DOI":"10.1165\/ajrcmb.25.3.4373","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T21:24:59Z","timestamp":1365024299000},"page":"285-290","source":"Crossref","is-referenced-by-count":19,"title":["Basic Electrical Properties of\n                    <i>In situ<\/i>\n                    Endothelial Cells of Small Pulmonary Arteries during Postnatal Development"],"prefix":"10.1093","volume":"25","author":[{"given":"Andrea","family":"Olschewski","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine; Department of Internal Medicine; Department of Physiology, Justus-Liebig-University, Giessen, Germany; and Instituto de Biologia Molecular e Celular, Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst","family":"Olschewski","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine; Department of Internal Medicine; Department of Physiology, Justus-Liebig-University, Giessen, Germany; and Instituto de Biologia Molecular e Celular, Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael E.","family":"Br\u00e4u","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine; Department of Internal Medicine; Department of Physiology, Justus-Liebig-University, Giessen, Germany; and Instituto de Biologia Molecular e Celular, Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunter","family":"Hempelmann","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine; Department of Internal Medicine; Department of Physiology, Justus-Liebig-University, Giessen, Germany; and Instituto de Biologia Molecular e Celular, Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Vogel","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine; Department of Internal Medicine; Department of Physiology, Justus-Liebig-University, Giessen, Germany; and Instituto de Biologia Molecular e Celular, Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris V.","family":"Safronov","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Intensive Care Medicine; Department of Internal Medicine; Department of Physiology, Justus-Liebig-University, Giessen, Germany; and Instituto de Biologia Molecular e Celular, Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,9,1]]},"reference":[{"key":"2026030700000004600_B1","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1126\/science.7268440","article-title":"Acetylcholine and bradykinin relax intrapulmonary arteries by acting on endothelial cells: role in lung vascular disease.","volume":"213","author":"Chand","year":"1981","journal-title":"Science"},{"key":"2026030700000004600_B2","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1111\/j.1540-8167.1994.tb01156.x","article-title":"The role of the membrane potential of endothelial and smooth muscle cells in the regulation of coronary blood flow.","volume":"5","author":"Daut","year":"1994","journal-title":"J. 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