{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:55:26Z","timestamp":1790434526849,"version":"4.1.0"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2001,9,1]],"date-time":"2001-09-01T00:00:00Z","timestamp":999302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Long-term, simultaneous, measurements of cytoplasmic free Ca2+ concentrations and single exocytotic fusion events in surfactant-secreting type II cells were performed. All fusion (constitutive, phorbol ester-induced, and agonist-induced) was Ca2+ -dependent. Kinetic analysis revealed that agonist (adenosine triphosphate [ATP])-induced fusion exhibited a kinetic pattern that correlated well with the Ca2+ signal. The effects of Ca2+ release from intracellular stores (early) and Ca2+ entry (late) could be demonstrated for the first time by dissecting the slow (10-to-15-min) fusion response to ATP into these two components. Bath Ba2+ or Sr2+ could replace Ca2+ to elicit a fusion response in thapsigargin-pretreated cells lacking ATP-induced Ca2+ release from stores. Although the late response was partially inhibited by interrupting the phospholipase D\u2013protein kinase C axis, a high Ca2+ dependence of the entire secretory course was demonstrated by a significant correlation between the integrated Ca2+ signal and the fusion response. There was also a highly significant correlation between constitutive and ATP-stimulated fusion activity in individual cells. We propose a common mechanistic model for all types of fusion in this slow secretory cell, in which constitutive and regulated forms of exocytosis are subject to the same principles of regulation.<\/jats:p>","DOI":"10.1165\/ajrcmb.25.3.4493","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T21:24:59Z","timestamp":1365024299000},"page":"306-315","source":"Crossref","is-referenced-by-count":59,"title":["Secretion in Alveolar Type II Cells at the Interface of Constitutive and Regulated Exocytosis"],"prefix":"10.1093","volume":"25","author":[{"given":"Manfred","family":"Frick","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Innsbruck, Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Eschertzhuber","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Innsbruck, Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Haller","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Innsbruck, Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norbert","family":"Mair","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Innsbruck, Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Dietl","sequence":"additional","affiliation":[{"name":"Department of Physiology, University of Innsbruck, Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,9,1]]},"reference":[{"key":"2026030700001671300_B1","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/0304-4157(91)90006-I","article-title":"Techniques and concepts in exocytosis: focus on mast cells.","volume":"1071","author":"Lindau","year":"1991","journal-title":"Biochim. 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