{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:17:05Z","timestamp":1790867825539,"version":"4.1.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2002,7,1]],"date-time":"2002-07-01T00:00:00Z","timestamp":1025481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Nitric oxide (NO) plays a major role in the modulation of perinatal pulmonary vascular tone. Congenital diaphragmatic hernia (CDH), a major cause of severe persistent pulmonary hypertension of the newborn (PPHN), is often refractory to inhaled NO. Alterations in NO\/cyclic guanosine 3\u2032 ,5\u2032 monophosphate (cGMP)-mediated pulmonary vasodilatation may contribute to PPHN in CDH. We assessed NO\/cGMP-mediated pulmonary vasorelaxation in vitro in 140-d gestational lamb fetuses with surgically created left CDH (term = 147 d) to age-matched controls. Relaxation of fourth generation intralobar pulmonary artery rings in response to the endothelium-dependent vasodilator, acetylcholine (ACh), and to the specific inhibitor of cGMP-phosphodiesterase (PDE), zaprinast, did not differ between the two groups. By contrast, relaxation in response to the calcium ionophore A23187 was impaired in CDH as compared with control animals. Relaxation in response to the NO donor sodium nitroprusside (SNP) (a direct activator of soluble guanylyl cyclase [sGC]) was also impaired in CDH animals as compared with controls. Repeating the challenge increased vasorelaxation in response to SNP in CDH as compared with control animals. Immunohistochemistry revealed the presence of endothelial NO-synthase in the endothelium of pulmonary arteries from both control and CDH animals. We conclude that endothelium-dependent vasodilatation in response to ACh and A23187 was differently affected in the fetal surgical CDH-lamb model. Furthermore, activity of sGC but not that of PDE was impaired in CDH animals. PPHN and decreased inhaled NO responsiveness in CDH may involve decreased sGC activity.<\/jats:p>","DOI":"10.1165\/ajrcmb.27.1.4712","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T21:19:47Z","timestamp":1365023987000},"page":"42-47","source":"Crossref","is-referenced-by-count":29,"title":["Altered Guanylyl-Cyclase Activity\n                    <i>In Vitro<\/i>\n                    of Pulmonary Arteries from Fetal Lambs with Congenital Diaphragmatic Hernia"],"prefix":"10.1093","volume":"27","author":[{"given":"Bernard","family":"Th\u00e9baud","sequence":"first","affiliation":[{"name":"Service de Physiologie\u2013Explorations Fonctionnelles, CHU Cochin, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris V, Paris; Service de R\u00e9animation N\u00e9onatale, H\u00f4pital Antoine B\u00e9cl\u00e8re, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris-Sud, Clamart; Service de Chirurgie Visc\u00e9rale Infantile, CHRU Caen, Caen; Service de Chirurgie Visc\u00e9rale P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 Paris VII, Paris; Ecole de Chirurgie, Assistance Publique-H\u00f4pitaux de Paris, Paris; and Service de R\u00e9animation P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris VII, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Petit","sequence":"additional","affiliation":[{"name":"Service de Physiologie\u2013Explorations Fonctionnelles, CHU Cochin, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris V, Paris; Service de R\u00e9animation N\u00e9onatale, H\u00f4pital Antoine B\u00e9cl\u00e8re, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris-Sud, Clamart; Service de Chirurgie Visc\u00e9rale Infantile, CHRU Caen, Caen; Service de Chirurgie Visc\u00e9rale P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 Paris VII, Paris; Ecole de Chirurgie, Assistance Publique-H\u00f4pitaux de Paris, Paris; and Service de R\u00e9animation P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris VII, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"de Lagausie","sequence":"additional","affiliation":[{"name":"Service de Physiologie\u2013Explorations Fonctionnelles, CHU Cochin, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris V, Paris; Service de R\u00e9animation N\u00e9onatale, H\u00f4pital Antoine B\u00e9cl\u00e8re, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris-Sud, Clamart; Service de Chirurgie Visc\u00e9rale Infantile, CHRU Caen, Caen; Service de Chirurgie Visc\u00e9rale P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 Paris VII, Paris; Ecole de Chirurgie, Assistance Publique-H\u00f4pitaux de Paris, Paris; and Service de R\u00e9animation P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris VII, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josette","family":"Dall'Ava-Santucci","sequence":"additional","affiliation":[{"name":"Service de Physiologie\u2013Explorations Fonctionnelles, CHU Cochin, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris V, Paris; Service de R\u00e9animation N\u00e9onatale, H\u00f4pital Antoine B\u00e9cl\u00e8re, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris-Sud, Clamart; Service de Chirurgie Visc\u00e9rale Infantile, CHRU Caen, Caen; Service de Chirurgie Visc\u00e9rale P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 Paris VII, Paris; Ecole de Chirurgie, Assistance Publique-H\u00f4pitaux de Paris, Paris; and Service de R\u00e9animation P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris VII, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Christophe","family":"Mercier","sequence":"additional","affiliation":[{"name":"Service de Physiologie\u2013Explorations Fonctionnelles, CHU Cochin, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris V, Paris; Service de R\u00e9animation N\u00e9onatale, H\u00f4pital Antoine B\u00e9cl\u00e8re, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris-Sud, Clamart; Service de Chirurgie Visc\u00e9rale Infantile, CHRU Caen, Caen; Service de Chirurgie Visc\u00e9rale P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 Paris VII, Paris; Ecole de Chirurgie, Assistance Publique-H\u00f4pitaux de Paris, Paris; and Service de R\u00e9animation P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris VII, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. Tuan","family":"Dinh-Xuan","sequence":"additional","affiliation":[{"name":"Service de Physiologie\u2013Explorations Fonctionnelles, CHU Cochin, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris V, Paris; Service de R\u00e9animation N\u00e9onatale, H\u00f4pital Antoine B\u00e9cl\u00e8re, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris-Sud, Clamart; Service de Chirurgie Visc\u00e9rale Infantile, CHRU Caen, Caen; Service de Chirurgie Visc\u00e9rale P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Universit\u00e9 Paris VII, Paris; Ecole de Chirurgie, Assistance Publique-H\u00f4pitaux de Paris, Paris; and Service de R\u00e9animation P\u00e9diatrique, H\u00f4pital Robert Debr\u00e9, Assistance Publique-H\u00f4pitaux de Paris, Universit\u00e9 Paris VII, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2002,7,1]]},"reference":[{"key":"2026030623545058200_B1","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1016\/S0095-5108(18)30201-X","article-title":"Congenital diaphragmatic hernia: epidemiology and outcome.","volume":"23","author":"Langham","year":"1996","journal-title":"Clin. 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