{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T23:15:35Z","timestamp":1774566935738,"version":"3.50.1"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Acute inflammation induced by intrapulmonary LPS requires nuclear factor (NF)-\u03baB RelA. This study elucidates the effects of intrapulmonary LPS on I\u03baB proteins, endogenous inhibitors of RelA, and the effects of deficiency of I\u03baB-\u03b2. I\u03baB-\u03b1, I\u03baB-\u03b2, and I\u03baB-\u03b5 each complexed with RelA in uninfected murine lungs. Intratracheal instillation of LPS induced the degradation of I\u03baB-\u03b1 and I\u03baB-\u03b2, as measured by the loss of immunoreactive proteins in non-nuclear fractions. Degradation was apparent by 2 h and sustained through 6 h. In contrast, net I\u03baB-\u03b5 content increased over this period. The small amounts of I\u03baB-\u03b1 and I\u03baB-\u03b2 that were detected in nuclear fractions from the lungs also decreased over this time frame, whereas intranuclear NF-\u03baB content (including both RelA and p50) increased. The hypophosphorylated form of I\u03baB-\u03b2, which facilitates transcription induced by NF-\u03baB, was not detected. Neutrophil recruitment and edema accumulation did not differ between wild type mice and gene-targeted mice deficient in I\u03baB-\u03b2, suggesting that I\u03baB-\u03b2 is not specifically required for these responses. Altogether, these data suggest that RelA is liberated during LPS-induced pulmonary inflammation by the regulated degradation of both I\u03baB-\u03b1 and I\u03baB-\u03b2. In the absence of I\u03baB-\u03b2, I\u03baB-\u03b1 or other inhibitory proteins can regulate NF-\u03baB functions essential to acute neutrophil emigration in the lungs.<\/jats:p>","DOI":"10.1165\/rcmb.2002-0015oc","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T21:09:04Z","timestamp":1365023344000},"page":"575-582","source":"Crossref","is-referenced-by-count":27,"title":["Functions of I\n                    <b>\u03ba<\/b>\n                    B Proteins in Inflammatory Responses to\n                    <i>Escherichia coli<\/i>\n                    LPS in Mouse Lungs"],"prefix":"10.1093","volume":"27","author":[{"given":"Joseph P.","family":"Mizgerd","sequence":"first","affiliation":[{"name":"Physiology Program, Harvard School of Public Health, Boston; and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin L.","family":"Scott","sequence":"additional","affiliation":[{"name":"Physiology Program, Harvard School of Public Health, Boston; and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matt R.","family":"Spieker","sequence":"additional","affiliation":[{"name":"Physiology Program, Harvard School of Public Health, Boston; and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire M.","family":"Doerschuk","sequence":"additional","affiliation":[{"name":"Physiology Program, Harvard School of Public Health, Boston; and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,11,1]]},"reference":[{"key":"2026030623553838800_BIB1","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1001\/jama.285.5.535","volume":"285","year":"2001","journal-title":"JAMA"},{"key":"2026030623553838800_BIB2","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1097\/00003246-199905000-00020","volume":"27","year":"1999","journal-title":"Crit. Care Med."},{"key":"2026030623553838800_BIB3","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1164\/ajrccm.160.3.9901107","volume":"160","year":"1999","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"2026030623553838800_BIB4","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1165\/ajrcmb.23.2.f189","volume":"23","year":"2000","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2026030623553838800_BIB5","doi-asserted-by":"crossref","first-page":"335","DOI":"10.4049\/jimmunol.154.1.335","volume":"154","year":"1995","journal-title":"J. Immunol."},{"key":"2026030623553838800_BIB6","doi-asserted-by":"crossref","first-page":"1963","DOI":"10.4049\/jimmunol.156.5.1963","volume":"156","year":"1996","journal-title":"J. Immunol."},{"key":"2026030623553838800_BIB7","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1093\/infdis\/173.1.159","volume":"173","year":"1996","journal-title":"J. Infect. Dis."},{"key":"2026030623553838800_BIB8","doi-asserted-by":"crossref","first-page":"5016","DOI":"10.4049\/jimmunol.157.11.5016","volume":"157","year":"1996","journal-title":"J. Immunol."},{"key":"2026030623553838800_BIB9","doi-asserted-by":"crossref","first-page":"2362","DOI":"10.1172\/JCI118679","volume":"97","year":"1996","journal-title":"J. Clin. Invest."},{"key":"2026030623553838800_BIB10","doi-asserted-by":"crossref","first-page":"4042","DOI":"10.4049\/jimmunol.166.6.4042","volume":"166","year":"2001","journal-title":"J. Immunol."},{"key":"2026030623553838800_BIB11","doi-asserted-by":"crossref","first-page":"6853","DOI":"10.1038\/sj.onc.1203239","volume":"18","year":"1999","journal-title":"Oncogene"},{"key":"2026030623553838800_BIB12","doi-asserted-by":"crossref","first-page":"L823","DOI":"10.1152\/ajpcell.1999.277.4.C823","volume":"277","year":"1999","journal-title":"Am. J. Physiol."},{"key":"2026030623553838800_BIB13","first-page":"85","volume":"22","year":"2000","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"2026030623553838800_BIB14","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.4049\/jimmunol.167.3.1592","volume":"167","year":"2001","journal-title":"J. Immunol."},{"key":"2026030623553838800_BIB15","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1146\/annurev.immunol.16.1.225","volume":"16","year":"1998","journal-title":"Annu. Rev. Immunol."},{"key":"2026030623553838800_BIB16","doi-asserted-by":"crossref","first-page":"6184","DOI":"10.1128\/MCB.17.10.6184","volume":"17","year":"1997","journal-title":"Mol. Cell. Biol."},{"key":"2026030623553838800_BIB17","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1073\/pnas.96.1.49","volume":"96","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026030623553838800_BIB18","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1093\/emboj\/16.6.1413","volume":"16","year":"1997","journal-title":"EMBO J."},{"key":"2026030623553838800_BIB19","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/0092-8674(95)90511-1","volume":"80","year":"1995","journal-title":"Cell"},{"key":"2026030623553838800_BIB20","doi-asserted-by":"crossref","first-page":"16317","DOI":"10.1074\/jbc.271.27.16317","volume":"271","year":"1996","journal-title":"J. Biol. Chem."},{"key":"2026030623553838800_BIB21","doi-asserted-by":"crossref","first-page":"23025","DOI":"10.1074\/jbc.272.37.23025","volume":"272","year":"1997","journal-title":"J. Biol. Chem."},{"key":"2026030623553838800_BIB22","doi-asserted-by":"crossref","first-page":"4221","DOI":"10.1128\/MCB.18.7.4221","volume":"18","year":"1998","journal-title":"Mol. Cell. Biol."},{"key":"2026030623553838800_BIB23","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1046\/j.1432-1327.1999.00028.x","volume":"259","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"2026030623553838800_BIB24","doi-asserted-by":"crossref","first-page":"6231","DOI":"10.1021\/bi990220t","volume":"38","year":"1999","journal-title":"Biochemistry"},{"key":"2026030623553838800_BIB25","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/S1097-2765(00)80445-1","volume":"5","year":"2000","journal-title":"Mol. Cell"},{"key":"2026030623553838800_BIB26","doi-asserted-by":"crossref","first-page":"2736","DOI":"10.1101\/gad.9.22.2736","volume":"9","year":"1995","journal-title":"Genes Dev."},{"key":"2026030623553838800_BIB27","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1084\/jem.188.6.1055","volume":"188","year":"1998","journal-title":"J. Exp. Med."},{"key":"2026030623553838800_BIB28","doi-asserted-by":"crossref","first-page":"6682","DOI":"10.1093\/emboj\/18.23.6682","volume":"18","year":"1999","journal-title":"EMBO J."},{"key":"2026030623553838800_BIB29","doi-asserted-by":"crossref","first-page":"2269","DOI":"10.1128\/MCB.20.6.2269-2284.2000","volume":"20","year":"2000","journal-title":"Mol. Cell. Biol."},{"key":"2026030623553838800_BIB30","first-page":"1014","volume":"USA 97","year":"2000","journal-title":"Proc. Nat. Acad. Sci"},{"key":"2026030623553838800_BIB31","doi-asserted-by":"crossref","first-page":"5444","DOI":"10.1128\/MCB.16.10.5444","volume":"16","year":"1996","journal-title":"Mol. Cell. Biol."},{"key":"2026030623553838800_BIB32","doi-asserted-by":"crossref","first-page":"4390","DOI":"10.1128\/MCB.17.8.4390","volume":"17","year":"1997","journal-title":"Mol. Cell. Biol."},{"key":"2026030623553838800_BIB33","doi-asserted-by":"crossref","first-page":"4737","DOI":"10.1128\/MCB.21.14.4737-4747.2001","volume":"21","year":"2001","journal-title":"Mol. Cell. Biol."},{"key":"2026030623553838800_BIB34","doi-asserted-by":"crossref","first-page":"L418","DOI":"10.1152\/ajplung.2001.281.2.L418","volume":"281","year":"2001","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"2026030623553838800_BIB35","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1084\/jem.186.8.1357","volume":"186","year":"1997","journal-title":"J. Exp. Med."},{"key":"2026030623553838800_BIB36","doi-asserted-by":"crossref","first-page":"13165","DOI":"10.1021\/bi972539o","volume":"37","year":"1998","journal-title":"Biochemistry"},{"key":"2026030623553838800_BIB37","doi-asserted-by":"crossref","first-page":"L400","DOI":"10.1152\/ajplung.2001.280.3.L400","volume":"280","year":"2001","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"2026030623553838800_BIB38","first-page":"1327","volume":"152","year":"1998","journal-title":"Am. J. Pathol."},{"key":"2026030623553838800_BIB39","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0002-9440(10)65270-4","volume":"154","year":"1999","journal-title":"Am. J. Pathol."},{"key":"2026030623553838800_BIB40","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1165\/ajrcmb.23.3.3949","volume":"23","year":"2000","journal-title":"Am. J. Respir. Cell Mol. Biol."}],"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/27\/5\/575\/67182155\/ajrcmb_27_5_575.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/27\/5\/575\/67182155\/ajrcmb_27_5_575.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T05:08:24Z","timestamp":1772860104000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/27\/5\/575\/8501700"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11,1]]},"references-count":40,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2002,11,1]]},"published-print":{"date-parts":[[2002,11,1]]}},"URL":"https:\/\/doi.org\/10.1165\/rcmb.2002-0015oc","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2002,11]]},"published":{"date-parts":[[2002,11,1]]}}}