{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:25:17Z","timestamp":1772929517948,"version":"3.50.1"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Endothelin-1 (ET-1) exerts many biological effects in airways, including bronchoconstriction, airway mucus secretion, cell proliferation, and inflammation. We investigated the effect of ET-1 on Na absorption and Cl secretion in human bronchial epithelial cells. Addition of 10\u22127 M ET-1 had no effect on the inhibition of the short circuit current (Isc) induced by amiloride, a Na channel blocker. Addition of 10\u22127 M ET-1 to the apical bath in the presence of amiloride increased Isc in cultured human bronchial epithelial cells studied in Ussing chambers. No effect was observed when ET-1 was added to basolateral bath, indicating that the involved ET-1 receptors are likely present only in the apical membrane of the cells. Use of Cl-free solutions and bumetanide reduced the ET-1\u2013induced increases in Isc, indicating that ET-1 stimulates Cl secretion. The ET-1\u2013induced increase in Isc was prevented by exposure to the ETB receptor antagonist BQ-788 but not to the ETA receptor antagonist BQ-123. ET-1 did not raise intracellular Ca levels, but increased the intracellular concentration of cAMP. These findings indicate that ET-1 is a Cl secretagogue in human airways and acts presumably through apically located ETB receptors and activation of the cAMP pathway.<\/jats:p>","DOI":"10.1165\/rcmb.2002-0104oc","type":"journal-article","created":{"date-parts":[[2003,3,10]],"date-time":"2003-03-10T21:07:06Z","timestamp":1047330426000},"page":"245-251","source":"Crossref","is-referenced-by-count":19,"title":["Effects of Endothelin-1 on Epithelial Ion Transport in Human Airways"],"prefix":"10.1093","volume":"29","author":[{"given":"Sabine","family":"Blouquit","sequence":"first","affiliation":[{"name":"Laboratoire de Biologie et Pharmacologie des Epith\u00e9liums Respiratoires, Boulogne; INSERM U339 UFR Saint Antoine, Paris; D\u00e9partement de Biophysique, University of Lille, Lille; Institut de Pharmacologie, Paris, France; and D\u00e9partement de Physiologie, University of Lille, Lille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anouar","family":"Sari","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie et Pharmacologie des Epith\u00e9liums Respiratoires, Boulogne; INSERM U339 UFR Saint Antoine, Paris; D\u00e9partement de Biophysique, University of Lille, Lille; Institut de Pharmacologie, Paris, France; and D\u00e9partement de Physiologie, University of Lille, Lille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Lombet","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie et Pharmacologie des Epith\u00e9liums Respiratoires, Boulogne; INSERM U339 UFR Saint Antoine, Paris; D\u00e9partement de Biophysique, University of Lille, Lille; Institut de Pharmacologie, Paris, France; and D\u00e9partement de Physiologie, University of Lille, Lille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"D'herbomez","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie et Pharmacologie des Epith\u00e9liums Respiratoires, Boulogne; INSERM U339 UFR Saint Antoine, Paris; D\u00e9partement de Biophysique, University of Lille, Lille; Institut de Pharmacologie, Paris, France; and D\u00e9partement de Physiologie, University of Lille, Lille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Naline","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie et Pharmacologie des Epith\u00e9liums Respiratoires, Boulogne; INSERM U339 UFR Saint Antoine, Paris; D\u00e9partement de Biophysique, University of Lille, Lille; Institut de Pharmacologie, Paris, France; and D\u00e9partement de Physiologie, University of Lille, Lille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regis","family":"Matran","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie et Pharmacologie des Epith\u00e9liums Respiratoires, Boulogne; INSERM U339 UFR Saint Antoine, Paris; D\u00e9partement de Biophysique, University of Lille, Lille; Institut de Pharmacologie, Paris, France; and D\u00e9partement de Physiologie, University of Lille, Lille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thierry","family":"Chinet","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie et Pharmacologie des Epith\u00e9liums Respiratoires, Boulogne; INSERM U339 UFR Saint Antoine, Paris; D\u00e9partement de Biophysique, University of Lille, Lille; Institut de Pharmacologie, Paris, France; and D\u00e9partement de Physiologie, University of Lille, Lille, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2003,8,1]]},"reference":[{"key":"2026030623511341100_BIB1","doi-asserted-by":"crossref","first-page":"2863","DOI":"10.1073\/pnas.86.8.2863","volume":"86","year":"1989","journal-title":"Proc. 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