{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:52:07Z","timestamp":1790887927695,"version":"4.1.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T00:00:00Z","timestamp":1080777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Human prostasin is a membrane-anchored serine peptidase hypothesized to regulate lung epithelial sodium transport. It belongs to a unique family of genes on chromosome 16p11.2\/13.3. Here we describe genomic cloning, promoter analysis, and expression of prostasin's mouse ortholog. The 4.3-kb mouse prostasin gene (prss8) has a six-exon organization identical to human prostasin. Prss8 spans two signal tagged-sites localized to chromosome 7. Multiple mRNA transcripts arise from two consensus initiator elements of a TATA-less promoter and an alternatively spliced, 5\u2032 untranslated region intron. Reporter assay establishes that the initiator elements and a GC-rich domain comprise the core promoter and identifies 5\u2032 flanking regions with strong enhancer and repressor activity. The 3\u2032 untranslated region overlaps the 3\u2032 untranslated region of the Myst1 gene oriented tail-to-tail at this locus. Prss8 is highly transcribed in pancreas, kidney, submaxillary gland, lung, thyroid, prostate, and epididymis, and is developmentally regulated. Using selective riboprobes and antibodies to mouse prostasin, we localized its expression to lung airway epithelial and alveolar type II cells and kidney cortical tubule epithelium. Mouse prostasin highly resembles its human ortholog in gene organization and tissue specificity, including strong expression in pulmonary epithelium, suggesting that mice will be useful for probing prostasin's functions in vivo.<\/jats:p>","DOI":"10.1165\/rcmb.2003-0251oc","type":"journal-article","created":{"date-parts":[[2003,9,8]],"date-time":"2003-09-08T22:31:13Z","timestamp":1063060273000},"page":"519-529","source":"Crossref","is-referenced-by-count":25,"title":["Mouse Prostasin Gene Structure, Promoter Analysis, and Restricted Expression in Lung and Kidney"],"prefix":"10.1093","volume":"30","author":[{"given":"George M.","family":"Verghese","sequence":"first","affiliation":[{"name":"Department of Medicine, University of Virginia, Charlottesville, Virginia; and Cardiovascular Research Institute, University of California, San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z. Y.","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Virginia, Charlottesville, Virginia; and Cardiovascular Research Institute, University of California, San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikash","family":"Bhagwandin","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Virginia, Charlottesville, Virginia; and Cardiovascular Research Institute, University of California, San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George H.","family":"Caughey","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Virginia, Charlottesville, Virginia; and Cardiovascular Research Institute, University of California, San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2004,4,1]]},"reference":[{"key":"2026030623485066300_BIB1","doi-asserted-by":"crossref","first-page":"3355","DOI":"10.1074\/jbc.274.6.3355","volume":"274","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2026030623485066300_BIB2","doi-asserted-by":"crossref","first-page":"6566","DOI":"10.4049\/jimmunol.164.12.6566","volume":"164","year":"2000","journal-title":"J. Immunol."},{"key":"2026030623485066300_BIB3","doi-asserted-by":"crossref","first-page":"3363","DOI":"10.1074\/jbc.M209353200","volume":"278","year":"2003","journal-title":"J. Biol. Chem."},{"key":"2026030623485066300_BIB4","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1006\/geno.1996.0127","volume":"32","year":"1996","journal-title":"Genomics"},{"key":"2026030623485066300_BIB5","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0167-4781(00)00071-3","volume":"1492","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"2026030623485066300_BIB6","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1006\/bbrc.1999.1870","volume":"266","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026030623485066300_BIB7","doi-asserted-by":"crossref","first-page":"49169","DOI":"10.1074\/jbc.M108677200","volume":"276","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2026030623485066300_BIB8","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0022-2836(88)90331-2","volume":"200","year":"1988","journal-title":"J. Mol. Biol."},{"key":"2026030623485066300_BIB9","doi-asserted-by":"crossref","first-page":"13483","DOI":"10.1074\/jbc.270.22.13483","volume":"270","year":"1995","journal-title":"J. Biol. Chem."},{"key":"2026030623485066300_BIB10","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1006\/bbrc.1998.9645","volume":"252","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026030623485066300_BIB11","first-page":"3199","volume":"59","year":"1999","journal-title":"Cancer Res."},{"key":"2026030623485066300_BIB12","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1681\/ASN.V115828","volume":"11","year":"2000","journal-title":"J. Am. Soc. Nephrol."},{"key":"2026030623485066300_BIB13","doi-asserted-by":"crossref","first-page":"8338","DOI":"10.1074\/jbc.M105044200","volume":"277","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2026030623485066300_BIB14","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1085\/jgp.20028598","volume":"120","year":"2002","journal-title":"J. Gen. Physiol."},{"key":"2026030623485066300_BIB15","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1007\/s00424-002-0955-1","volume":"445","year":"2003","journal-title":"Pflugers Arch."},{"key":"2026030623485066300_BIB16","first-page":"2207","volume":"93","year":"2003","journal-title":"J. Appl. Physiol."},{"key":"2026030623485066300_BIB17","first-page":"L16","volume":"281","year":"2001","journal-title":"Am. J. Physiol."},{"key":"2026030623485066300_BIB18","doi-asserted-by":"crossref","first-page":"R1031","DOI":"10.1152\/ajpcell.00474.2002","volume":"284","year":"2003","journal-title":"Am. J. Physiol."},{"key":"2026030623485066300_BIB19","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1002\/ijc.1601","volume":"97","year":"2002","journal-title":"Int. J. Cancer"},{"key":"2026030623485066300_BIB20","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1002\/pros.1085","volume":"48","year":"2001","journal-title":"Prostate"},{"key":"2026030623485066300_BIB21","doi-asserted-by":"crossref","first-page":"41906","DOI":"10.1074\/jbc.M205868200","volume":"277","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2026030623485066300_BIB22"},{"key":"2026030623485066300_BIB23","doi-asserted-by":"crossref","first-page":"3023","DOI":"10.1093\/nar\/24.15.3023","volume":"24","year":"1996","journal-title":"Nucleic Acids Res."},{"key":"2026030623485066300_BIB24","first-page":"L134","volume":"258","year":"1990","journal-title":"Am. J. Physiol."},{"key":"2026030623485066300_BIB25","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1093\/bioinformatics\/14.1.25","volume":"14","year":"1998","journal-title":"Bioinformatics"},{"key":"2026030623485066300_BIB26","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1172\/JCI0213229","volume":"109","year":"2002","journal-title":"J. Clin. Invest."},{"key":"2026030623485066300_BIB27","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1146\/annurev.bi.50.070181.002025","volume":"50","year":"1981","journal-title":"Annu. Rev. Biochem."},{"key":"2026030623485066300_BIB28","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/ng1001-201","volume":"29","year":"2001","journal-title":"Nat. Genet."},{"key":"2026030623485066300_BIB29","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/S0167-4781(99)00211-0","volume":"1490","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"2026030623485066300_BIB30","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0092-8674(89)90176-1","volume":"57","year":"1989","journal-title":"Cell"},{"key":"2026030623485066300_BIB31","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1161\/01.HYP.0000013055.48885.8D","volume":"39","year":"2002","journal-title":"Hypertension"},{"key":"2026030623485066300_BIB32","doi-asserted-by":"crossref","first-page":"30784","DOI":"10.1074\/jbc.274.43.30784","volume":"274","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2026030623485066300_BIB33","doi-asserted-by":"crossref","first-page":"1250","DOI":"10.1046\/j.1432-1327.2001.01986.x","volume":"268","year":"2001","journal-title":"Eur. J. Biochem."},{"key":"2026030623485066300_BIB34","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/S0167-4781(99)00226-2","volume":"1490","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"2026030623485066300_BIB35","doi-asserted-by":"crossref","first-page":"20648","DOI":"10.1074\/jbc.M010422200","volume":"276","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2026030623485066300_BIB36","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1681\/ASN.V1261114","volume":"12","year":"2001","journal-title":"J. Am. Soc. Nephrol."},{"key":"2026030623485066300_BIB37","doi-asserted-by":"crossref","first-page":"2712","DOI":"10.1073\/pnas.051603198","volume":"98","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026030623485066300_BIB38"},{"key":"2026030623485066300_BIB39","doi-asserted-by":"crossref","first-page":"3948","DOI":"10.1073\/pnas.90.9.3948","volume":"90","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026030623485066300_BIB40","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1046\/j.1432-0436.1998.6410011.x","volume":"64","year":"1998","journal-title":"Differentiation"},{"key":"2026030623485066300_BIB41","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.1242\/dev.129.11.2619","volume":"129","year":"2002","journal-title":"Development"},{"key":"2026030623485066300_BIB42","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0014-5793(97)01465-8","volume":"419","year":"1997","journal-title":"FEBS Lett."},{"key":"2026030623485066300_BIB43","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1023\/A:1007652414175","volume":"15","year":"1999","journal-title":"Cell Biol. Toxicol."},{"key":"2026030623485066300_BIB44","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/S0006-2952(02)01153-X","volume":"64","year":"2002","journal-title":"Biochem. Pharmacol."},{"key":"2026030623485066300_BIB45","first-page":"L1258","volume":"280","year":"2001","journal-title":"Am. J. Physiol."},{"key":"2026030623485066300_BIB46","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1080\/01902140290103062","volume":"28","year":"2002","journal-title":"Exp. Lung Res."},{"key":"2026030623485066300_BIB47","first-page":"L1186","volume":"278","year":"2000","journal-title":"Am. J. Physiol."},{"key":"2026030623485066300_BIB48","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1101\/gr.191702","volume":"12","year":"2002","journal-title":"Genome Res."},{"key":"2026030623485066300_BIB49","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1210\/endo.142.5.8170","volume":"142","year":"2001","journal-title":"Endocrinology"},{"key":"2026030623485066300_BIB50"},{"key":"2026030623485066300_BIB51","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1165\/ajrcmb.20.3.3283","volume":"20","year":"1999","journal-title":"Am. J. Respir. Cell Mol. Biol."}],"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/30\/4\/519\/67151450\/ajrcmb_30_4_519.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/30\/4\/519\/67151450\/ajrcmb_30_4_519.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T05:00:37Z","timestamp":1772859637000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/30\/4\/519\/8500840"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4,1]]},"references-count":51,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2004,4,1]]},"published-print":{"date-parts":[[2004,4,1]]}},"URL":"https:\/\/doi.org\/10.1165\/rcmb.2003-0251oc","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2004,4]]},"published":{"date-parts":[[2004,4,1]]}}}