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In exposed workers, genetic susceptibility to this granulomatous disorder is associated with particular HLA-DPB1 alleles. We hypothesized that these HLA-DP molecules could mediate Be-stimulated tumor necrosis factor-\u03b1 (TNF-\u03b1) messenger RNA (mRNA) and protein production. Using intracellular cytokine staining, we found that treatment with an anti\u2013HLA-DP, but not anti\u2013HLA-DR, monoclonal antibody inhibited Be-stimulated TNF-\u03b1 expression in lung CD3+ CD4+ T cells. This monoclonal antibody also blocked Be-specific T cell proliferation, increased production of TNF-\u03b1 mature-mRNA transcripts, and increased TNF-\u03b1 protein production by Be-stimulated CBD peripheral blood mononuclear cells and bronchoalveolar lavage (BAL) cells. The Be-stimulated upregulation of TNF-\u03b1 mature-mRNA levels with TNF-\u03b1 protein production was a unique property of CBD BAL cells, and did not occur in BAL cells from Be-sensitized patients without CBD, or sarcoidosis BAL cells. This study identifies HLA-DP as a regulatory component in the activation of T cell receptors on Be-specific CD4+ T cells from CBD patients resulting in proliferation and proinflammatory cytokine production.<\/jats:p>","DOI":"10.1165\/rcmb.2003-0336oc","type":"journal-article","created":{"date-parts":[[2004,2,23]],"date-time":"2004-02-23T20:53:01Z","timestamp":1077569581000},"page":"122-130","source":"Crossref","is-referenced-by-count":29,"title":["Beryllium-Induced Tumor Necrosis Factor-\u03b1 Production by CD4+ T Cells Is Mediated by HLA-DP"],"prefix":"10.1093","volume":"31","author":[{"given":"Richard T.","family":"Sawyer","sequence":"first","affiliation":[{"name":"Department of Medicine, Hollis Laboratory of Environmental and Occupational Health, National Jewish Medical and Research Center, Denver; and Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles E.","family":"Parsons","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hollis Laboratory of Environmental and Occupational Health, National Jewish Medical and Research Center, Denver; and Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew P.","family":"Fontenot","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hollis Laboratory of Environmental and Occupational Health, National Jewish Medical and Research Center, Denver; and Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa A.","family":"Maier","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hollis Laboratory of Environmental and Occupational Health, National Jewish Medical and Research Center, Denver; and Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"May M.","family":"Gillespie","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hollis Laboratory of Environmental and Occupational Health, National Jewish Medical and Research Center, Denver; and Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. 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