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Lungs were dissected into upper and lower lobe peripheral sections for RNA extraction. Microarray analysis was performed using Affymetrix Hu-133 Plus 2.0 arrays. We observed that the relative variability of gene expression increased rapidly from technical (lowest), to regional, to population (highest). In addition, age and sex have measurable effects on gene expression. Gene expression variability is heterogeneously distributed among biologic categories. We conclude that gene expression variability is greater between individuals than within individuals and that population variability is the most important factor in the study design of microarray experiments of the human lung. Classes of genes with high population variability are biologically important and provide a novel perspective into lung physiology and pathobiology. Our study represents the first comprehensive analysis of nondiseased lung tissue. The generation of this robust dataset has important implications for the design and implementation of future comparative expression analysis with pulmonary disease states.<\/jats:p>","DOI":"10.1165\/rcmb.2004-0261oc","type":"journal-article","created":{"date-parts":[[2006,2,23]],"date-time":"2006-02-23T20:59:50Z","timestamp":1140728390000},"page":"65-71","source":"Crossref","is-referenced-by-count":31,"title":["Human Lung Project: Evaluating Variance of Gene Expression in the Human Lung"],"prefix":"10.1093","volume":"35","author":[{"given":"Michael P.","family":"Gruber","sequence":"first","affiliation":[{"name":"Division of Pulmonary Sciences and Critical Care Medicine and Section of Biometrics and Informatics, University of Colorado Health Sciences Center; and National Jewish Medical and Research Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher D.","family":"Coldren","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Sciences and Critical Care Medicine and Section of Biometrics and Informatics, University of Colorado Health Sciences Center; and National Jewish Medical and Research Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malcolm D.","family":"Woolum","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Sciences and Critical Care Medicine and Section of Biometrics and Informatics, University of Colorado Health Sciences Center; and National Jewish Medical and Research Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory P.","family":"Cosgrove","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Sciences and Critical Care Medicine and Section of Biometrics and Informatics, University of Colorado Health Sciences Center; and National Jewish Medical and Research Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chan","family":"Zeng","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Sciences and Critical Care Medicine and Section of Biometrics and Informatics, University of Colorado Health Sciences Center; and National Jewish Medical and Research Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna E.","family":"Bar\u00f3n","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Sciences and Critical Care Medicine and Section of Biometrics and Informatics, University of Colorado Health Sciences Center; and National Jewish Medical and Research Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark D.","family":"Moore","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Sciences and Critical Care Medicine and Section of Biometrics and Informatics, University of Colorado Health Sciences Center; and National Jewish Medical and Research Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlyne D.","family":"Cool","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Sciences and Critical Care Medicine and Section of Biometrics and Informatics, University of Colorado Health Sciences Center; and National Jewish Medical and Research Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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