{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:17:21Z","timestamp":1772929041994,"version":"3.50.1"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2006,4,1]],"date-time":"2006-04-01T00:00:00Z","timestamp":1143849600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Chronic exposure of human isolated bronchi to \u03b22-adrenergic agonists, especially fenoterol, potentiates smooth muscle contraction in response to endothelin-1 (ET-1), a peptide implicated in chronic inflammatory airway diseases. Our objective was to determine whether ET-1 receptors ETA and ETB are involved in fenoterol enhancement. Twenty-two human bronchi were sensitized to ET-1 by prolonged incubation with 0.1 \u03bcM fenoterol (15 h, 21\u00b0C). Removing the epithelium after fenoterol incubation limited the maximal contraction (0.10 \u00b1 0.36 g without epithelium versus 1.18 \u00b1 0.22 with, n = 8, P = 0.04). After 15 h incubation, 14 and 8 paired rings were fixed, respectively, for immunolabeling of bronchial ETA and ETB receptors, and to determine the mRNA expression levels using real-time quantitative reverse transcription polymerase chain reaction. ETA and ETB receptor mRNA expressions were 1.27- \u00b1 0.14-fold (not significant) and 2.24- \u00b1 0.28-fold (P &amp;lt; 0.01) higher, respectively, in fenoterol-treated bronchi than in paired controls. Fenoterol incubation significantly increased epithelial ETA and ETB receptor labeling intensity scores (P = 0.001 and P = 0.002, respectively, versus controls), and enhanced the diffuse localization of ETA receptors on the epithelial cells (P = 0.002 versus controls), but did not change the ETB-receptor immunolabeling intensity on airway smooth muscle. We conclude that fenoterol-induced sensitization of human isolated bronchi involves epithelial ETA and ETB receptors, which suggests perturbation of the epithelial regulation of airway smooth muscle contraction in response to ET-1.<\/jats:p>","DOI":"10.1165\/rcmb.2005-0091oc","type":"journal-article","created":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T20:44:39Z","timestamp":1134161079000},"page":"410-416","source":"Crossref","is-referenced-by-count":13,"title":["\u03b22-Adrenoceptor Agonist Modulates Endothelin-1 Receptors in Human Isolated Bronchi"],"prefix":"10.1093","volume":"34","author":[{"given":"Christophe","family":"Faisy","sequence":"first","affiliation":[{"name":"UPRES EA220, Facult\u00e9 de M\u00e9decine Paris-Ouest and UFR Biom\u00e9dicale des Saints-P\u00e8res, and Service de R\u00e9animation M\u00e9dicale, H\u00f4pital Europ\u00e9en Georges Pompidou, Paris, France; and Centro de Investigaciones Cientificas Isla de la Cartuja, Instituto de Investigaciones 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