{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T09:24:57Z","timestamp":1789809897778,"version":"4.0.1"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2005,10,1]],"date-time":"2005-10-01T00:00:00Z","timestamp":1128124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We synthesized and assessed the role of a diacylglycerol (DAG)-containing docosahexaenoic acid (DHA), that is, 1-stearoyl-2-docosahexaenoyl-sn-glycerol (SDHG), in the contraction of guinea pig airway smooth muscle (ASM). We compared its action with 1-stearoyl-2-arachidonoyl-sn-glycerol (SAG) and 1,2-dioctanoyl-sn-glycerol (1,2-DiC8), a stable DAG analog. The three DAGs (SAG, SDHG, and 1,2-DiC8) induced reversible concentration-dependent contraction of ASM. SDHG induced higher guinea pig ASM contraction than did SAG and 1,2-DiC8. The effects of SDHG were blocked, to different extents, by nifedipine (L-type Ca2+ channel blocker). By employing GF-109203X (protein kinase C [PKC] inhibitor) and lanthanum (La3+), a nonselective cation channel blocker, we observed that SDHG evoked ASM contractile response via PKC-dependent and PKC-independent (but Ca2+-dependent) pathways. Interestingly, SAG exerted its action only by increasing [Ca2+]i and did not require PKC activation. To probe the implication of calcium mobilization, we employed thapsigargin (TG), which also induced ASM contraction in a calcium-dependent manner. SDHG and 1,2-DiC8, in a PKC-dependent manner, induced the phosphorylation of CPI-17 (myosin light chain phosphatase inhibitor of 17 kD). Furthermore, SAG and TG failed to phosphorylate CPI-17 in ASM cells. Our results suggest that different DAG species, produced during a dietary supplementation with fatty acids, could modulate the reactivity of airway smooth muscles in a PKC-dependent and -independent manner, and hence, may play a critical role in health and disease.<\/jats:p>","DOI":"10.1165\/rcmb.2005-0136oc","type":"journal-article","created":{"date-parts":[[2005,6,16]],"date-time":"2005-06-16T20:56:11Z","timestamp":1118955371000},"page":"378-386","source":"Crossref","is-referenced-by-count":13,"title":["Diacylglycerol-Containing Docosahexaenoic Acid in Acyl Chain Modulates Airway Smooth Muscle Tone"],"prefix":"10.1093","volume":"33","author":[{"given":"Aziz","family":"Hichami","sequence":"first","affiliation":[{"name":"D\u00e9partement\u2014Physiologie, Immunologie et Neurosciences, Unit\u00e9 Propre de Recherche de l'Enseignement Sup\u00e9rieur\u2014Lipides et Nutrition, Facult\u00e9 des Sciences de la Vie, Universit\u00e9 de Bourgogne, Dijon, France; and Le Bilarium, Department of Physiology and Biophysics, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Morin","sequence":"additional","affiliation":[{"name":"D\u00e9partement\u2014Physiologie, Immunologie et Neurosciences, Unit\u00e9 Propre de Recherche de l'Enseignement Sup\u00e9rieur\u2014Lipides et Nutrition, Facult\u00e9 des Sciences de la Vie, Universit\u00e9 de Bourgogne, Dijon, France; and Le Bilarium, Department of Physiology and Biophysics, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Rousseau","sequence":"additional","affiliation":[{"name":"D\u00e9partement\u2014Physiologie, Immunologie et Neurosciences, Unit\u00e9 Propre de Recherche de l'Enseignement Sup\u00e9rieur\u2014Lipides et Nutrition, Facult\u00e9 des Sciences de la Vie, Universit\u00e9 de Bourgogne, Dijon, France; and Le Bilarium, Department of Physiology and Biophysics, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naim A.","family":"Khan","sequence":"additional","affiliation":[{"name":"D\u00e9partement\u2014Physiologie, Immunologie et Neurosciences, Unit\u00e9 Propre de Recherche de l'Enseignement Sup\u00e9rieur\u2014Lipides et Nutrition, Facult\u00e9 des Sciences de la Vie, Universit\u00e9 de Bourgogne, Dijon, France; and Le Bilarium, Department of Physiology and Biophysics, Faculty of Medicine, Universit\u00e9 de Sherbrooke, Sherbrooke, Quebec, 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