{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:19:00Z","timestamp":1772929140414,"version":"3.50.1"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2005,9,1]],"date-time":"2005-09-01T00:00:00Z","timestamp":1125532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Eosinophils are an important source of leukotriene (LT)C4, which can be synthesized within lipid bodies\u2014cytoplasmic organelles where eicosanoid formation may take place. Allergy-driven lipid body formation and function have never been investigated. Here, we studied the in vivo induction and role of lipid bodies within eosinophils recruited to sites of allergic inflammation. Using two murine models of allergic inflammation (asthma and pleurisy), we verified that parallel to the eosinophil influx, allergic challenge also induced lipid body formation within recruited eosinophils. Neutralizing antibodies to eotaxin\/CCL11, RANTES\/CCL5, or CCR3 partially inhibited lipid body formation within recruited eosinophils in the allergic pleurisy model. Likewise, intrapleural administration of RANTES or eotaxin also induced significant influx of eosinophils loaded with lipid bodies. By immunolabeling, we detected the presence of a key enzyme involved in the leukotriene metabolism\u20145-lipoxygenase\u2014within eosinophil lipid bodies formed in vivo after allergen challenge. Furthermore, specific immunolocalization of newly formed LTC4 demonstrated that lipid bodies were the sites of formation of this eicosanoid within infiltrating eosinophils. Therefore, allergic inflammation triggers in vivo formation of new lipid bodies within infiltrating eosinophils, a phenomenon largely mediated by eotaxin\/RANTES acting via CCR3 receptors. Such in vivo allergen-driven lipid bodies function as intracellular compartments of LTC4 synthesis.<\/jats:p>","DOI":"10.1165\/rcmb.2005-0145oc","type":"journal-article","created":{"date-parts":[[2005,6,9]],"date-time":"2005-06-09T20:45:58Z","timestamp":1118349958000},"page":"254-261","source":"Crossref","is-referenced-by-count":42,"title":["Allergic Challenge\u2013Elicited Lipid Bodies Compartmentalize\n                    <i>In Vivo<\/i>\n                    Leukotriene C4 Synthesis within Eosinophils"],"prefix":"10.1093","volume":"33","author":[{"given":"Adriana","family":"Vieira-de-Abreu","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Imunofarmacologia, Departamento de Fisiologia e Farmacodin\u00e2mica, Instituto Oswaldo Cruz, Funda\u00e7a\u00f5 Oswaldo Cruz, Rio de Janeiro, Brazil; and Department of Medicine, Harvard Medical School, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edson F.","family":"Assis","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunofarmacologia, Departamento de Fisiologia e Farmacodin\u00e2mica, Instituto Oswaldo Cruz, Funda\u00e7a\u00f5 Oswaldo Cruz, Rio de Janeiro, Brazil; and Department of Medicine, Harvard Medical School, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gleice S.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunofarmacologia, Departamento de Fisiologia e Farmacodin\u00e2mica, Instituto Oswaldo Cruz, Funda\u00e7a\u00f5 Oswaldo Cruz, Rio de Janeiro, Brazil; and Department of Medicine, Harvard Medical School, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hugo C.","family":"Castro-Faria-Neto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunofarmacologia, Departamento de Fisiologia e Farmacodin\u00e2mica, Instituto Oswaldo Cruz, Funda\u00e7a\u00f5 Oswaldo Cruz, Rio de Janeiro, Brazil; and Department of Medicine, Harvard Medical School, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter F.","family":"Weller","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunofarmacologia, Departamento de Fisiologia e Farmacodin\u00e2mica, Instituto Oswaldo Cruz, Funda\u00e7a\u00f5 Oswaldo Cruz, Rio de Janeiro, Brazil; and Department of Medicine, Harvard Medical School, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christianne","family":"Bandeira-Melo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunofarmacologia, Departamento de Fisiologia e Farmacodin\u00e2mica, Instituto Oswaldo Cruz, Funda\u00e7a\u00f5 Oswaldo Cruz, Rio de Janeiro, Brazil; and Department of Medicine, Harvard Medical School, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia T.","family":"Bozza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunofarmacologia, Departamento de Fisiologia e Farmacodin\u00e2mica, Instituto Oswaldo Cruz, Funda\u00e7a\u00f5 Oswaldo Cruz, Rio de Janeiro, Brazil; and Department of Medicine, Harvard Medical School, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2005,9,1]]},"reference":[{"key":"2026030623431371100_BIB1","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1016\/S0091-6749(99)70069-2","volume":"104","year":"1999","journal-title":"J Allergy Clin Immunol"},{"key":"2026030623431371100_BIB2","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/S0091-6749(97)70237-9","volume":"100","year":"1997","journal-title":"J Allergy Clin 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