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To study this possibility, we developed an in vitro system in which we collected ASL from primary cultures of normal and CF airway epithelial cells. Microbial killing was less efficient when bacteria were incubated with neutrophils in the presence of ASL from CF epithelia compared with normal ASL. Antimicrobial functions of human neutrophils were assessed in ASL from CF and normal epithelia using a combination of quantitative bacterial culture, flow cytometry, and microfluorescence imaging. The results of these assays of neutrophil function were indistinguishable in CF and normal ASL. In contrast, the direct bactericidal activity of ASL to Escherichia coli and to clinical isolates of Staphylococcus aureus and Pseudomonas aeruginosa was substantially less in CF than in normal ASL, even when highly diluted in media of identical ionic strength. Together, these observations indicate that the antimicrobial properties of ASL in CF are compromised in a manner independent of ionic strength of the ASL, and that this effect is not mediated through a direct effect of the ASL on phagocyte function.<\/jats:p>","DOI":"10.1165\/rcmb.2005-0146oc","type":"journal-article","created":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T20:43:54Z","timestamp":1132260234000},"page":"364-374","source":"Crossref","is-referenced-by-count":30,"title":["Abnormalities in the Pulmonary Innate Immune System in Cystic Fibrosis"],"prefix":"10.1093","volume":"34","author":[{"given":"Theo J.","family":"Moraes","sequence":"first","affiliation":[{"name":"Division of Respirology, Department of Medicine, University of Toronto; Toronto General Hospital; Toronto Western Hospital Divisions of the Research Institute of the University Health Network; Division of Pediatric Respirology, and The Research Institute, Hospital for Sick Children, Toronto, Ontario, 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