{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:03:25Z","timestamp":1790982205767,"version":"4.1.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2006,8,1]],"date-time":"2006-08-01T00:00:00Z","timestamp":1154390400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Previous studies have suggested that the proinflammatory cytokine, TNF-\u03b1, contributes to airway hyperresponsivness by altering airway smooth muscle (ASM) Ca2+ responses to agonist stimulation. The present study examined the effects of TNF-\u03b1 on Ca2+ influx pathways in cultured human ASM cells (HASMCs). Proteins encoded by the transient receptor potential (TRP) gene family function as channels through which receptor-operated and store-operated Ca2+ entry (SOCE) occur. In the present study, the presence of TRPC1, TRPC3, TRPC4, TRPC5, and TRPC6 mRNA and protein expression was confirmed in cultured HASMCs using RT-PCR and Western blot analysis. TNF-\u03b1 treatment significantly increased TRPC3 mRNA and protein levels in HASMCs as well as SOCE. TNF-\u03b1 treatment also increased both the peak and plateau intracellular Ca2+ concentration responses in HASMCs elicited by acetylcholine and bradykinin. The effects of TNF-\u03b1 treatment on SOCE and agonist-induced intracellular Ca2+ concentration responses were attenuated using small interfering RNA transfection, which knocked down TRPC3 expression. Thus, in inflammatory airway diseases, TNF-\u03b1 treatment may result in increased myocyte activation due to altered Ca2+ influx pathways. These results suggest that TRPC3 may be an important therapeutic target in inflammatory airway diseases such as asthma and chronic obstructive pulmonary disease.<\/jats:p>","DOI":"10.1165\/rcmb.2006-0003oc","type":"journal-article","created":{"date-parts":[[2006,3,30]],"date-time":"2006-03-30T20:59:25Z","timestamp":1143752365000},"page":"243-251","source":"Crossref","is-referenced-by-count":112,"title":["Role of Transient Receptor Potential C3 in TNF-\u03b1\u2013Enhanced Calcium Influx in Human Airway Myocytes"],"prefix":"10.1093","volume":"35","author":[{"given":"Thomas A.","family":"White","sequence":"first","affiliation":[{"name":"Departments of Allergy and Immunology, Medicine, Anesthesiology, and Physiology and Biomedical Engineering, College of Medicine, Mayo Clinic, Rochester, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailing","family":"Xue","sequence":"additional","affiliation":[{"name":"Departments of Allergy and Immunology, Medicine, Anesthesiology, and Physiology and Biomedical Engineering, College of Medicine, Mayo Clinic, Rochester, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo N.","family":"Chini","sequence":"additional","affiliation":[{"name":"Departments of Allergy and Immunology, Medicine, Anesthesiology, and Physiology and Biomedical Engineering, College of Medicine, Mayo Clinic, Rochester, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Thompson","sequence":"additional","affiliation":[{"name":"Departments of Allergy and Immunology, Medicine, Anesthesiology, and Physiology and Biomedical Engineering, College of Medicine, Mayo Clinic, Rochester, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary C.","family":"Sieck","sequence":"additional","affiliation":[{"name":"Departments of Allergy and Immunology, Medicine, Anesthesiology, and Physiology and Biomedical Engineering, College of Medicine, Mayo Clinic, Rochester, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark E.","family":"Wylam","sequence":"additional","affiliation":[{"name":"Departments of Allergy and Immunology, Medicine, Anesthesiology, and Physiology and Biomedical Engineering, College of Medicine, Mayo Clinic, Rochester, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2006,8,1]]},"reference":[{"key":"2026030623394087600_bib1","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1016\/0091-6749(92)90218-Q","volume":"89","year":"1992","journal-title":"J Allergy Clin Immunol"},{"key":"2026030623394087600_bib2","first-page":"643","volume":"40","year":"1991","journal-title":"Arerugi"},{"key":"2026030623394087600_bib3","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1111\/j.1365-2222.1995.tb03249.x","volume":"25","year":"1995","journal-title":"Clin Exp Allergy"},{"key":"2026030623394087600_bib4","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1164\/ajrccm.152.1.7599866","volume":"152","year":"1995","journal-title":"Am J Respir Crit Care Med"},{"key":"2026030623394087600_bib5","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1046\/j.1440-1711.2001.00980.x","volume":"79","year":"2001","journal-title":"Immunol Cell Biol"},{"key":"2026030623394087600_bib6","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1136\/thx.2003.019588","volume":"59","year":"2004","journal-title":"Thorax"},{"key":"2026030623394087600_bib7","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/S1359-6101(03)00058-3","volume":"14","year":"2003","journal-title":"Cytokine Growth Factor Rev"},{"key":"2026030623394087600_bib8","first-page":"1489","volume":"316","year":"1993","journal-title":"C R Acad Sci III"},{"key":"2026030623394087600_bib9","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1111\/j.1476-5381.1995.tb14896.x","volume":"114","year":"1995","journal-title":"Br J Pharmacol"},{"key":"2026030623394087600_bib10","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1124\/mol.63.3.714","volume":"63","year":"2003","journal-title":"Mol Pharmacol"},{"key":"2026030623394087600_bib11","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1111\/j.1469-7793.1999.0561p.x","volume":"518","year":"1999","journal-title":"J Physiol"},{"key":"2026030623394087600_bib12","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1046\/j.1440-1843.2001.00334.x","volume":"6","year":"2001","journal-title":"Respirology"},{"key":"2026030623394087600_bib13","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1046\/j.1440-1843.2000.00240.x","volume":"5","year":"2000","journal-title":"Respirology"},{"key":"2026030623394087600_bib14","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1152\/japplphysiol.00140.2003","volume":"95","year":"2003","journal-title":"J Appl Physiol"},{"key":"2026030623394087600_bib15","first-page":"L468","volume":"259","year":"1990","journal-title":"Am J Physiol"},{"key":"2026030623394087600_bib16","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1113\/jphysiol.1991.sp018501","volume":"435","year":"1991","journal-title":"J Physiol"},{"key":"2026030623394087600_bib17","doi-asserted-by":"crossref","first-page":"L909","DOI":"10.1152\/ajplung.00317.2003","volume":"286","year":"2004","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2026030623394087600_bib18","first-page":"1","volume":"7","year":"1986","journal-title":"Cell Calcium"},{"key":"2026030623394087600_bib19","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1042\/bj2580409","volume":"258","year":"1989","journal-title":"Biochem J"},{"key":"2026030623394087600_bib20","first-page":"387","volume":"2","year":"2001","journal-title":"Nat Rev Neurosci"},{"key":"2026030623394087600_bib21","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1002\/(SICI)1521-1878(199901)21:1<38::AID-BIES5>3.0.CO;2-S","volume":"21","year":"1999","journal-title":"Bioessays"},{"key":"2026030623394087600_bib22","doi-asserted-by":"crossref","first-page":"33980","DOI":"10.1074\/jbc.C100321200","volume":"276","year":"2001","journal-title":"J Biol Chem"},{"key":"2026030623394087600_bib23","doi-asserted-by":"crossref","first-page":"21649","DOI":"10.1074\/jbc.M302162200","volume":"278","year":"2003","journal-title":"J Biol Chem"},{"key":"2026030623394087600_bib24","doi-asserted-by":"crossref","first-page":"S183","DOI":"10.2337\/diabetes.51.2007.S183","volume":"51","year":"2002","journal-title":"Diabetes"},{"key":"2026030623394087600_bib25","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1165\/rcmb.2003-0134OC","volume":"30","year":"2004","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2026030623394087600_bib26","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1164\/ajrccm.162.2.9911025","volume":"162","year":"2000","journal-title":"Am J Respir Crit Care Med"},{"key":"2026030623394087600_bib27","doi-asserted-by":"crossref","first-page":"L1020","DOI":"10.1152\/ajpcell.1997.273.3.C1020","volume":"273","year":"1997","journal-title":"Am J Physiol"},{"key":"2026030623394087600_bib28","first-page":"452","volume":"17","year":"2003","journal-title":"FASEB J"},{"key":"2026030623394087600_bib29","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1097\/00000542-200408000-00018","volume":"101","year":"2004","journal-title":"Anesthesiology"},{"key":"2026030623394087600_bib30","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1165\/rcmb.2005-0187OC","volume":"34","year":"2006","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2026030623394087600_bib31","first-page":"L1","volume":"272","year":"1997","journal-title":"Am J Physiol"},{"key":"2026030623394087600_bib32","first-page":"L197","volume":"276","year":"1999","journal-title":"Am J Physiol"},{"key":"2026030623394087600_bib33","doi-asserted-by":"crossref","first-page":"L1091","DOI":"10.1152\/ajplung.2000.278.5.L1091","volume":"278","year":"2000","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2026030623394087600_bib34","first-page":"482","volume":"17","year":"2003","journal-title":"FASEB J"},{"key":"2026030623394087600_bib35","doi-asserted-by":"crossref","first-page":"21617","DOI":"10.1074\/jbc.M202549200","volume":"277","year":"2002","journal-title":"J Biol Chem"},{"key":"2026030623394087600_bib36","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1042\/bj20020061","volume":"364","year":"2002","journal-title":"Biochem J"},{"key":"2026030623394087600_bib37","first-page":"145","volume":"30","year":"2003","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2026030623394087600_bib38","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1038\/35888","volume":"391","year":"1998","journal-title":"Nature"},{"key":"2026030623394087600_bib39","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/nrg908","volume":"3","year":"2002","journal-title":"Nat Rev Genet"},{"key":"2026030623394087600_bib40","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1164\/ajrccm\/145.2_Pt_1.332","volume":"145","year":"1992","journal-title":"Am Rev Respir Dis"},{"key":"2026030623394087600_bib41","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1136\/thx.2005.045260","volume":"60","year":"2005","journal-title":"Thorax"},{"key":"2026030623394087600_bib42","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1046\/j.1440-1843.2003.00424.x","volume":"8","year":"2003","journal-title":"Respirology"},{"key":"2026030623394087600_bib43","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1152\/japplphysiol.00722.2001","volume":"92","year":"2002","journal-title":"J Appl Physiol"},{"key":"2026030623394087600_bib44","doi-asserted-by":"crossref","first-page":"L909","DOI":"10.1152\/ajplung.00317.2003","volume":"286","year":"2004","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2026030623394087600_bib45","doi-asserted-by":"crossref","first-page":"C485","DOI":"10.1152\/ajpcell.1993.264.2.C485","volume":"264","year":"1993","journal-title":"Am J Physiol"},{"key":"2026030623394087600_bib46","doi-asserted-by":"crossref","first-page":"L1303","DOI":"10.1152\/ajplung.00240.2004","volume":"287","year":"2004","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2026030623394087600_bib47","doi-asserted-by":"crossref","first-page":"37195","DOI":"10.1074\/jbc.M304287200","volume":"278","year":"2003","journal-title":"J Biol Chem"}],"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/35\/2\/243\/67086857\/ajrcmb_35_2_243.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/35\/2\/243\/67086857\/ajrcmb_35_2_243.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T04:50:37Z","timestamp":1772859037000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/35\/2\/243\/8493623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8,1]]},"references-count":47,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2006,8,1]]},"published-print":{"date-parts":[[2006,8,1]]}},"URL":"https:\/\/doi.org\/10.1165\/rcmb.2006-0003oc","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2006,8]]},"published":{"date-parts":[[2006,8,1]]}}}